Bioremediation of hydrocarbons in soil is a highly complex process, involving a multiplicity of physical, chemical and biological phenomena. Therefore, it is extremely difficult to control and boost the bioremediation of these systems after an oil spill. A mathematical model was developed to assist in the prediction and decision-making regarding the in situ bioremediation of hydrocarbon-contaminated soils. The ...
Waste and wastewater containing hydrocarbons are produced worldwide by various oil-based industries, whose activities also contribute to the occurrence of oil spills throughout the globe, causing severe environmental contamination. Anaerobic microorganisms with the ability to biodegrade petroleum hydrocarbons are important in the treatment of contaminated matrices, both in situ in deep subsurfaces, or ex situ i...
Oil and gas industry is responsible for the generation of large volumes of oil-contaminated wastewaters, such as saline produced water (PW), that without proper treatment can cause environmental contamination. Alcanivorax borkumensis SK2 is a biosurfactant producer capable of degrading and converting hydrocarbons into bacterial lipids under nutrient limiting conditions (e.g. nitrogen and/or oxygen). Recently, t...
Oilfield produced water (PW) management and treatment represents a significant cost to oil producers. Alcanivorax borkumensis is a marine bacteria capable of converting hydrocarbons into storage lipids, currently of great interest as raw materials for biofuel or oleochemical industries. In this study, the effect of dissolved oxygen (DO) concentration on the production of storage compounds by A. borkumensis SK2 ...
Produced water (PW) and spent oil-based wastewaters are some of the largest mineral oil wastewaters produced. Due to the high toxicity of hydrocarbons, several countries set stringent discharge limits and its treatment is compulsory before discharge. In this work, biological treatment of mineral oil wastewaters coupled with the production of bacterial lipids is demonstrated in sequential batch airlift reactors ...
Microbial communities with the ability to convert long-chain fatty acids (LCFA) coupled to sulfate reduction can be important in the removal of these compounds from wastewater. In this work, an enrichment culture, able to oxidize the long-chain fatty acid palmitate (C16:0) coupled to sulfate reduction, was obtained from anaerobic granular sludge. Microscopic analysis of this culture, designated HP culture, reve...
Petroleum-based oily wastes are generated by the oil industry and can be treated/valorized by anaerobic microbial conversion to methane. However, this process is generally slow. Conductive nanomaterials were reported to accelerate the interspecieselectron transfer in anaerobic communities and therefore their addition to anaerobic processes treating hydrocarbons may also be advantageous. In this work, two ferric...
It is clear that aerobic and facultative anaerobic bacteria have an important role in the first steps of the anaerobic digestion (AD) process, especially when complex organic compounds are degraded. However, their diversity, abundance and function, related to the fine control of process variables such as pH and ORP, and the potential establishment of networks with methanogens and acetogens are far from being fu...
Regranulated cork particles are by-products of cork stopper production, which present very high hydrophobicity and oil sorption capacity after thermal treatment [1]. These thermally treated granules have been used as absorbents in the remediation of oil spills under the commercial brand Corksorb (Corticeira Amorim, S.G.P.S.). Once saturated with oil, cork should be regenerated for cyclic reuse. For that, hydroc...
Bacterial storage lipids are being considered as viable alternative feedstocks for industrial and biotechnological applications, compared to conventional ones. The production of these bacterial compounds can be obtained from different carbon sources, including inexpensive and recalcitrant wastes. This thesis explores the potential of using hydrocarbonoclastic bacteria to obtain lipid reserve substances from hyd...