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Specificity and sensitivity comparative study between phage PVP-S1 and monoclon...

Oliveira, T. V. de; Soares, N. de F. F.; Carvalho, Carla A. O. C. M.; Coimbra, J. S. dos R.; Andrade, N. J. de; Azeredo, Joana; Medeiros, E. A. A.

Polydiacetylene polymer (PDA) has been intensively studied because of its properties as colour change from blue to red and change from non-fluorescent to fluorescent form due to an external stimulus that lead to a reorientation of the PDA within the organized structure. External stimulus could be temperature, pH, solvent influence, bacteria presence, mechanical stresses and others (Oliveira et al., 2012). Pires...


Correction: Population dynamics of a salmonella lytic phage and its host: impli...

Santos, Sílvio Roberto Branco; Carvalho, Carla A. O. C. M.; Azeredo, Joana; Ferreira, Eugénio C.

The Fig 1 Figure Legend is incorrect. The authors have provided a corrected version here.


A bacteriophage detection tool for viability assessment of Salmonella cells

Fernandes, Elisabete Ramos; Martins, V. C.; Nóbrega, Cláudia; Carvalho, Carla A. O. C. M.; Cardoso, F. A.; Cardoso, S.; Dias, J.; Deng, D.

Salmonellosis, one of the most common food and water-borne diseases, has a major global health and economic impact. Salmonella cells present high infection rates, persistence over inauspicious conditions and the potential to preserve virulence in dormant states when cells are viable but non-culturable (VBNC). These facts are challenging for current detection methods. Culture methods lack the capacity to detect ...


Population dynamics of a salmonella lytic phage and its host: implications of t...

Santos, Sílvio Roberto Branco; Carvalho, Carla A. O. C. M.; Azeredo, Joana; Ferreira, Eugénio C.

The prevalence and impact of bacteriophages in the ecology of bacterial communities coupled with their ability to control pathogens turn essential to understand and predict the dynamics between phage and bacteria populations. To achieve this knowledge it is essential to develop mathematical models able to explain and simulate the population dynamics of phage and bacteria. We have developed an unstructured mathe...


Spintronic devices for biomedical applications

Freitas, P. P.; Cardoso, S.; Cardoso, F. A.; Dias, T.; Martins, V. C.; Fernandes, Elisabete Ramos; Carvalho, Carla A. O. C. M.; Azeredo, Joana

Spintronic devices have been proposed over the past decade for various biomedical applications. These include static or dynamic biomolecular recognition platforms ( DNA-cDNA, antibody-antigen, phage-bacteria, ), cytometer and cell separation devices and lateral bio assay platforms , microelectrode based devices for neuroelectronic applications, and hybrid sensor arrays for imaging applications [1]. The biomolec...


Development of a predictive model for phage-bacteria interaction

Santos, Sílvio Roberto Branco; Carvalho, Carla A. O. C. M.; Azeredo, Joana; Ferreira, Eugénio C.

The prevalence and impact of bacteriophages in the ecology of bacterial communities coupled with their ability to control pathogens turns essential to understand and predict the existing dynamics between phage and bacteria populations. The success of phage therapy is dependent on the knowledge of the phage replication kinetic properties in the presence of the host as well as on the prediction, controlling and o...


The in vivo efficacy of two administration routes of a phage cocktail to reduce...

Carvalho, Carla A. O. C. M.; Gannon, B. W.; Halfhide, D. E.; Santos, Sílvio Roberto Branco; Hayes, C. M.; Roe, J. M.; Azeredo, Joana

Background Poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. Campylobacter coli and Campylobacter jejuni are the most common Campylobacter species associated with this disease. These pathogens live in the intestinal tract of most avian species and under commercial conditions they spread rapidly to infect a high proport...


Characterization and in vivo efficacy of a T4 bacteriophage to reduce numbers o...

Carvalho, Carla A. O. C. M.; Gannon, B.; Kropinski, Andrew M.; Azeredo, Joana


Genome of campylobacter coli bacteriophage phiCcoIBB_35

Carvalho, Carla A. O. C. M.; Langohr, Erika; Kropinski, Andrew M.; Azeredo, Joana

Campylobacter is recognized worldwide as the major etiologic agent in human diarrheoal disease, being Campylobacter jejuni and Campylobacter coli the most prevalent species. Bacteriophages are natural predators of bacteria, ubiquitous in the environment, self-limiting, self- replicating and with a high host-specicity. These make them potentially an important biocontrol agent of foodborne diseases. There are onl...


Method for bacteriophage isolation against target Campylobacter strains

Carvalho, Carla A. O. C. M.; Susano, M.; Fernandes, Elisabete Ramos; Santos, Sílvio Roberto Branco; Gannon, B. W.; Nicolau, Ana; Gibbs, Paul

Aims: Poultry meat is considered a major source of Campylobacter. This micro-aerobic bacterium is commonly responsible for foodborne illness. This work focuses on the isolation of Campylobacter coli lytic bacteriophages (phages) against target C. coli strains. Methods and Results: A method involving the enrichment of free-range chicken samples in a broth containing the target C. coli strains and salts (CaCl2 an...


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