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Discriminating between typical and atypical cystic fibrosis-related bacterial s...
Lopes, Susana P.; Carvalho, Daniel T.; Pereira, Maria Olívia; Azevedo, Nuno F.This study aims to report the development of peptide nucleic acid (PNA) probes to specifically detect the cystic fibrosis (CF)-associated traditional and atypical species Pseudomonas aeruginosa and Inquilinus limosus, respectively. PNA probes were designed in silico, developed and tested in smears prepared in phosphate-buffer saline and in artificial sputum medium. A multiplex fluorescent in situ hybridization ...
Date: 2017
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Origin: RepositóriUM - Universidade do Minho