16 documents found, page 1 of 2
Leishmania infantum in the reproductive organs of dogs
Silva,Diogo Tiago da; Alves,Maria Luana; Spada,Julio Cesar Pereira; Leonel,João Augusto Franco; Benassi,Julia Cristina; Starke-Buzetti,Wilma AparecidaABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. infantum in the reproductive organs of male and female dogs. Using histochemistry, immunohistochemistry, and PCR, we examined tissue samples from the reproductive organs of 8 male dogs and 16 female dogs diagnosed with leishmaniasis. Despite the absence of macroscopic or microscopic lesions ...
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil ...
Sevá,Anaiá da Paixão; Brandão,Ana Pérola Drulla; Godoy,Silvia Neri; Soares,Rodrigo Martins; Langoni,Helio; Rodrigues,Bruna CristineAbstract INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling in...
Sarcocystis neurona and related Sarcocystis spp. shed by opossums (Didelphis sp...
Gondim,Luís Fernando Pita; Soares,Rodrigo Martins; Moré,Gastón; Jesus,Rogério Fernando de; Llano,Horwald Alexander BedoyaAbstract Protozoan parasites of the genus Sarcocystis are obligatory heteroxenous cyst-forming coccidia that infect a wide variety of animals and encompass approximately 200 described species. At least four Sarcocystis spp. (S. falcatula, S. neurona, S. lindsayi and S. speeri) use opossums (Didelphis spp.) as definitive hosts, and two of them, S. neurona and S. falcatula, are known to cause disease in horses an...
Synanthropic rodents as virus reservoirs and transmitters
Gravinatti,Mara Lucia; Barbosa,Carla Meneguin; Soares,Rodrigo Martins; Gregori,FábioAbstract This review focuses on reports of hepatitis E virus, hantavirus, rotavirus, coronavirus, and arenavirus in synanthropic rodents (Rattus rattus, Rattus norvegicus, and Mus musculus) within urban environments. Despite their potential impact on human health, relatively few studies have addressed the monitoring of these viruses in rodents. Comprehensive control and preventive activities should include acti...
Molecular detection of Leishmania infantum DNA according to clinical stages of ...
Fernandes,Murilo Antônio; Leonel,João Augusto Franco; Isaac,Jéssica Anzolin; Benassi,Julia Cristina; Silva,Diogo Tiago; Spada,Julio Cesar PereiraAbstract The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) of dogs were classified as without clinical signs, 67.5% ...
Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR us...
Vioti,Geovanna; Leonel,João Augusto Franco; Lemes,Kleber Menegon; Pereira,Vanessa Figueredo; Ferreira,Helena Lage; Keid,Lara BorgesAbstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers spe...
Endoparasites in domestic animals surrounding an Atlantic Forest remnant, in Sã...
Sevá,Anaiá da Paixão; Pena,Hilda Fátima de Jesus; Nava,Alessandra; Sousa,Amanda Oliveira de; Holsback,Luciane; Soares,Rodrigo MartinsAbstract Morro do Diabo State Park (MDSP) is a significant remnant of the Atlantic Rain Forest in Brazil and is surrounded by rural properties. In that area, wild and domestic animals and humans are in close contact, which facilitates the two-way flow of infectious diseases among them. We assessed endoparasites in domestic livestock from all rural properties surrounding MDSP. There were sampled 197 cattle, 37 h...
Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine popul...
Lima,Julia Teresa Ribeiro de; Gennari,Solange Maria; Soares,Herbert Sousa; Minervino,Antonio Humberto Hamad; Malheiros,Antonio FranciscoABSTRACT INTRODUCTION: Leishmaniasis is endemic to the Northern, Northeastern, Central-Western, and Southeastern regions of Brazil. We aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the States of Mato Grosso and Tocantins using a serological survey conducted in May 2011. METHODS: Serum samples were collected from 470 humans and 327 dogs living...
Molecular phylogeny of Toxoplasmatinae: comparison between inferences based on ...
Sercundes,Michelle Klein; Valadas,Samantha Yuri Oshiro Branco; Keid,Lara Borges; Oliveira,Tricia Maria Ferreira Souza; Ferreira,Helena LageAbstract Phylogenies within Toxoplasmatinae have been widely investigated with different molecular markers. Here, we studied molecular phylogenies of the Toxoplasmatinae subfamily based on apicoplast and mitochondrial genes. Partial sequences of apicoplast genes coding for caseinolytic protease (clpC) and beta subunit of RNA polymerase (rpoB), and mitochondrial gene coding for cytochrome B (cytB) were analyzed....
Evidence of heterozygosity and recombinant alleles in single cysts of Giardia d...
Aguiar,Juliana Martins; Silva,Sheila Oliveira; Santos,Valdir Azevedo dos; Taniwaki,Sueli Akemi; Oliveira,Tricia Maria Ferreira de SousaAbstract Giardia duodenalis is divided into eight assemblages (named A to H). Isolates of assemblage A are divided into four sub-assemblages (AI, AII, AIII and AIV). While isolates of sub-assemblage AII are almost exclusively detected in human hosts, isolates of assemblage B are encountered in a multitude of animal hosts and humans. Here, we isolated single cysts of G. duodenalis from a human stool sample and f...