6 documents found, page 1 of 1
Longitudinally aligned inner-patterned silk fibroin conduits for peripheral ner...
Escobar Fernandez, Ane; Carvalho, Mariana R.; Silva, Tiago H.; Reis, R. L.; Oliveira, Joaquim M.Peripheral nerve injuries represent a major clinical challenge, if nerve ends retract, there is no spontaneous regeneration, and grafts are required to proximate the nerve ends and give continuity to the nerve. The nerve guidance conduits (NGCs) presented in this work are silk fibroin (SF)-based, which is biocompatible and very versatile. The formation of conduits is obtained by forming a covalently cross-linke...
Synthesis of mussel-inspired polydopamine-gallium nanoparticles for biomedical ...
Teixeira, Jean Valdir Uchôa; Maia, F. Raquel; Carvalho, Mariana R.; Reis, R. L.; Oliveira, J. M.; Lisboa-Filho, Paulo NoronhaAim: In this work, we established a simple, controlled, and reproducible method to synthesize gallium-coated polydopamine (PDA) nanoparticles. Methods: Polydopamine was synthetized in the alkali medium with posterior gallium shell formation due to ion chelation on the nanoparticle surface. Results: The obtained results with EDS spectra confirmed the incorporation of gallium on PDA surface. The cytotoxicity of G...
Anti-cancer drug validation: the contribution of tissue engineered models
Carvalho, Mariana R.; Lima, Daniela; Reis, R. L.; Oliveira, J. M.; Correlo, V. M.Abstract Drug toxicity frequently goes concealed until clinical trials stage, which is the most challenging, dangerous and expensive stage of drug development. Both the cultures of cancer cells in traditional 2D assays and animal studies have limitations that cannot ever be unraveled by improvements in drug-testing protocols. A new generation of bioengineered tumors is now emerging in response to these limitati...
A semiautomated microfluidic platform for real-time investigation of nanopartic...
Carvalho, Mariana R.; Maia, F. Raquel; Silva-Correia, Joana; Costa, Bruno Marques; Reis, R. L.; Oliveira, J. M.Aims: develop a platform composed of labeled dendrimer nanoparticles and a microfluidic device for real-time monitoring of cancer cells fate. Materials and Methods: The physicochemical and biological characterization of the developed Carboxymethyl-chitosan/poly(amidoamine) (CMCht/PAMAM) dendrimer nanoparticles were performed using TEM, AFM, Zeta Sizer, DSC and cytotoxicity screening. Cancer cell lines derived f...
Gellan gum-coated gold nanorods: an intracellular nanosystem for bone tissue en...
Vieira, Sílvia; Vial, Stéphanie; Maia, F. Raquel; Carvalho, Mariana R.; Reis, R. L.; Granja, Pedro L.; Oliveira, Joaquim Miguel Antunes CorreiaGold nanorods (AuNRs) have emerged as an exceptional nanotool for a myriad of applications ranging from cancer therapy to tissue engineering. However, their surface modification with biocompatible and stabilizing biomaterials is crucial to allow their use in a biological environment. Herein, low-acyl gellan gum (GG) was used to coat AuNRs surface, taking advantage of its stabilizing, biocompatible and gelling f...
Evaluating biomaterial-and microfluidic-based 3D tumor models
Carvalho, Mariana R.; Lima, Daniela; Reis, R. L.; Correlo, V. M.; Oliveira, J. M.Cancer is a major cause of morbidity and mortality worldwide, with a disease burden estimated to increase in the coming decades. Disease heterogeneity and limited information on cancer biology and disease mechanisms are aspects that 2D cell cultures fail to address. We review the current "state-of-the-art" in 3D Tissue Engineering (TE) models developed for and used in cancer research. Scaffold-based TE models a...