18 documents found, page 1 of 2
Platelet-derived products in veterinary medicine: A new trend or an effective t...
Soares, Carla S.; Babo, Pedro Miguel Sousa; Reis, R. L.; Carvalho, P. P.; Gomes, Manuela E.Platelet-derived products (PDPs) have gained popularity, mainly due to their high concentrations of bioactive molecules such as growth factors and cytokines, which play important roles in tissue healing and regeneration. PDPs are obtained through minimally invasive procedures and their therapeutic effect has been widely recognized. In veterinary medicine, however, the lack of standard protocols to generate PDPs...
Evaluation of hematology, general serum biochemistry, bone turnover markers and...
Coelho, Catarina A.; Bordelo, João P.; Camassa, José A.; Barros, Vera A.; Babo, Pedro Miguel Sousa; Gomes, Manuela E.; Reis, R. L.; Azevedo, J. T.Osteoporosis is a metabolic disorder characterized by a loss of bone mass and structure and increasing the risk of fragility fractures, mostly among postmenopausal women. Sheep is a recognized large animal model for osteoporosis research. An experimental group of ewes (3-4 years old) was subjected to ovariectomy (OVX) and weekly glucocorticoid (GC) application for 24 weeks and compared with a sham control group...
Evaluation of canine adipose-derived stem cells in a healthy mice subcutaneous ...
Requicha, J. F.; Carvalho, P. P.; Pires, M. A.; Dias, M. I.; Reis, R. L.; Gomes, Manuela E.; Viegas, C. A. A.Canine adipose-derived stem cells (cASCs) have great interest for cell-based therapies in Veterinary Medicine. As the behaviour of these cells in non-autologous recipients is not deeply characterized, it is mandatory to study them in new animal models previously to canine specie. In this work, cASCs were injected subcutaneously in mice and these cells were detected by immunohistochemistry using vimentin, CD44 a...
Starch-based blends in tissue engineering
Carvalho, P. P.; Rodrigues, Márcia T.; Gomes, Manuela E.; Reis, R. L.As the world's second most abundant biopolymer, and being relatively inexpensive to attain, starch serves as food, feed and renewable resource for bioenergy production and other industrial applications. The rising development of processing techniques and starch's broadly tailorable properties caught the attention of researchers for its application in the biomedical field, particularly in bone, cartilage, and ...
Exploring the potential of starch/polycaprolactone aligned magnetic responsive ...
Gonçalves, A. I.; Rodrigues, M. T.; Carvalho, P. P.; Bañobre-López, M.; Paz, E.; Freitas, P.; Gomes, Manuela E.The application of magnetic nanoparticles (MNPs) in tissue engineering (TE) approaches opens several new research possibilities in this field, enabling a new generation of multifunctional constructs for tissue regeneration. This study describes the development of sophisticated magnetic polymer scaffolds with aligned structural features aimed at applications in tendon tissue engineering (TTE). Tissue engineering...
Assessment of bone healing ability of calcium phosphate cements loaded with pla...
Babo, P. S.; Carvalho, P. P.; Santo, Vítor Sérgio Soares Espírito; Faria, Susana; Gomes, Manuela E.; Reis, R. L.Injectable calcium phosphate cements have been used as a valid alternative to autologous bone grafts for bone augmentation with the additional advantage of enabling minimally invasive implantation procedures and for perfectly fitting the tissue defect. Nevertheless, they have low biodegradability and lack adequate biochemical signaling to promote bone healing and remodeling. In previous in vitro studies, we ob...
Comparison of infrapatellar and subcutaneous adipose tissue stromal vascular fr...
Carvalho, P. P.; Hamel, Katie M.; Duarte, Robert; King, Andrew G. S.; Haque, Masudul; Dietrich, Marilyn A.; Wu, Xiying; Shah, Forum; Burk, DavidSince inflammatory mechanisms have been postulated to link obesity to osteoarthritis, the current study evaluated the ratio of immune cells to multipotent stromal cells within the infrapatellar fat pad (IPFP) and subcutaneous adipose tissue (SQ) of the knee; each depot has potential as a source of regenerative cells. The immunophenotypes of stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs) o...
Evaluation of the in vitro and in vivo biocompatibility of carrageenan based hy...
Popa, Elena Geta; Carvalho, P. P.; Dias, A. F.; Santos, T. C.; Santo, Vitor E.; Marques, A. P.; Viegas, C. A.; Dias, Isabel R.; Gomes, M. E.Carrageenans are highly sulphated galactans, well-known for their thermogelation properties which have been extensively exploited in food and cosmetics industry but poorly explored in the biomedicine field. In this study we have assessed thein vitroandin vivobiocompatibility of κ-carrageenan hydrogels that have been explored for regenerative medicine and tissue engineering applications. Thein vitrocytotoxicity ...
Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun ...
Carvalho, P. P.; Leonor, I. B.; Smith, Brenda J.; Dias, Isabel R.; Reis, R. L.; Gimble, Jeffrey M.; Gomes, Manuela E.The repair of large bony defects remains challenging in the clinical setting. Human adipose-derived stromal/ stem cells (hASCs) have been reported to differentiate along different cell lineages, including the osteogenic. The objective of the present study was to assess the bone regeneration potential of undifferentiated hASCs loaded in starchpolycaprolactone (SPCL) scaffolds, in a critical-sized nude mice calva...
Chondrogenic potential of two hASCs subpopulations loaded onto gellan gum hydro...
Rada, Tommaso; Carvalho, P. P.; Santos, T. C.; Castro, António G.; Reis, R. L.; Gomes, Manuela E.Cells constitute one of the fundamental components of any cartilage tissue engineering approach. Adipose tissue derived stem cells (ASCs) have a promising future considering the abundance of this tissue in the human body, ease of harness, and the high number of stem cells that can be isolated from small amounts of tissue. However the stromal vascular fraction of the adipose tissue that is isolated upon digestio...