17 documents found, page 1 of 2
Nanobubble-enhanced oxygen transfer in bacterial nanocellulose production: comp...
Rodrigues, Ana Cristina Costa; Martins, Daniela Sofia Rodrigues; Carvalho, Ricardo Emanuel Silva; Marques, Susana Cristina Gomes; Belo, IsabelDespite the unique properties of bacterial nanocellulose (BNC), oxygen limitation during large-scale production impairs microbial metabolism and cellulose synthesis, leading to high production costs and limited commercial success. Static fermentation can achieve high titers, but industrially it is operationally challenging. Agitated systems like airlift (AL) bioreactors, allow faster production but typically yi...
Optimizing bacterial nanocellulose production from eucalyptus bark: a circular ...
Rodrigues, Ana Cristina Costa; Martins, Daniela Sofia Rodrigues; Duarte, Maria Salomé Lira; Carvalho, Ricardo Emanuel Silva; Marques, SusanaThe production cost of bacterial nanocellulose (BNC) is a major limitation to its widespread use. However, this limitation can be addressed by using alternative low-cost substrates and high-yield strains. Agro-industrial waste-derived substrates offer a cost-effective and sustainable solution, but their high organic load often requires additional downstream wastewater treatments. Here, we optimized static BNC p...
Laser‑patterning bacterial nanocellulose for cell‑controlled interaction
Costa, Lígia; Carvalho, Alexandre F.; Carvalho, Ricardo Emanuel Silva; Rodrigues, Ana Cristina; Dourado, Fernando; Deuermeier, Jonas; Neto, Miguel A.The interfacial topography of biomaterials has been identified as a major biophysical regulator of cell behavior and function, a role played through the interplay with biochemical cues. In this work, we demonstrate the potential of laser as a versatile technology for the direct fine-tuning of the topography of Bacterial nanocellulose (BNC) with bioinspired topographies and micropatterns on a cell size scale. Tw...
TGFβ in malignant canine mammary tumors: relation with angiogenesis, immunologi...
Carvalho, Maria Isabel; Carvalho, Ricardo Emanuel Silva; Prada, Justina; Pinto, Carla; Gregório, Hugo; Lobo, Luis; Pires, Isabel; Queiroga, Felisbina L.Transforming growth factor- (TGF) and FoxP3 regulatory T cells (Treg) are involved in human breast carcinogenesis. This topic is not well documented in canine mammary tumors (CMT). In this work, the tumoral TGF expression was assessed by immunohistochemistry in 67 malignant CMT and its correlation to previously determined FoxP3, VEGF, and CD31 markers and other clinicopathologic parameters was evaluated. The hi...
Bacterial cellulose in vitro uptake by macrophages, epithelial cells, and a tri...
Carvalho, Ricardo Emanuel Silva; Rodrigues, Patrícia M.; Martins, Daniela Sofia Rodrigues; Rodrigues, Ana Cristina Costa; Sampaio, PaulaBacterial cellulose (BC) has a long-standing human consumption history in different geographies without any report of adverse effects. Despite its unique textural and functional properties, the use of BC in food products in Europe is still restricted due to concerns over its nanosize. Here, we evaluated the potential uptake of celluloses (from plant and microbial sources, processed using different blenders) by ...
Effect of bacterial cellulose on the foaming properties of egg white and soy pr...
Martins, Daniela Sofia Rodrigues; Khodamoradi, Niloofar; Carvalho, Ricardo Emanuel Silva; Gama, F. M.; Moradi, Mehran; Dourado, FernandoFood foams are highly industrially relevant systems, responsible for the visual appearance and organoleptic properties of many processed foods. Foam characteristics can be further improved by food-grade additives. This study investigates the influence of bacterial cellulose (BC) on the foaming capacity (FC) and foam stability (FS) of egg white protein (EWP) and soy protein isolate (SPI), important food foaming ...
Amorphous polysaccharide-drug formulations for intramacrophagic treatment of in...
Carvalho, Ricardo Emanuel SilvaO tratamento da leishmaniose é normalmente baseado em formulações de anfotericina B (AmB), que apresentam sérias limitações. Apesar dos progressos realizados nesta área, continua a ser necessário desenvolver alternativas menos dispendiosas e menos tóxicas. Esta tese tem como objetivo desenvolver um processo simples e de baixo custo para produzir nanocomplexos de AmB-polissacarídeo solúveis em água, com atividad...
Covalent conjugation of amphotericin B to hyaluronic acid: an injectable water-...
Carvalho, Ricardo Emanuel Silva; Leão, Teresa; Gama, F. M.; Tomás, Ana M.Amphotericin B (AmB) is a highly hydrophobic drug with significant leishmanicidal activity whose use is limited by its poor water solubility and adverse effects. Polymer-drug conjugates are proposed as a delivery system designed to overcome those limitations while improving drug bioavailability, safety, and activity. Here, AmB was covalently linked to periodate-oxidized hyaluronic acid (HA) (oxidation degree of...
Hyaluronic acidamphotericin B nanocomplexes: a promising anti-leishmanial drug ...
Carvalho, Ricardo Emanuel Silva; Leão, T.; Bourbon, A. I.; Gonçalves, Catarina; Pastrana, L. M.; Parpot, Pier; Amorim, I.; Tomás, A. M.; Gama, F. M.The development of an effective amphotericin B (AmB) formulation to replace actual treatments available for leishmaniasis, which present serious drawbacks, is a challenge. Here we report the development of hyaluronic acidamphotericin B self-assembled nanocomplexes (HAAmB), processed by freeze-drying (FD) or nano spray-drying (SD), using a simple process that favors the non-covalent drugpolysaccharide associatio...
Corrigendum to “Development of dextrin-amphotericin B formulations for the trea...
Carvalho, Ricardo Emanuel Silva; Fidalgo, J.; Melo, K. R.; Queiroz, M. F.; Leal, Ana Salomé Barbosa; Rocha, H. A.; Cruz, T.; Parpot, Pier; Tomás, A. M.The authors regret to inform that there are some inaccuracies in the above referred article. More specifically, in the end of the Results and Discussion section 3.6. Anti-Leishmania activity against axenic promastigote and intramacrophagic L. infantum amastigotes it is described the following “Dex-AmB nanocomplexes had an activity similar to the free-drug, despite the slightly higher IC50 values - 0.017 ± 0.009...