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Optimization of a quantitative PCR methodology for detection of Aspergillus spp...
Mendonca, Alexandre; Carvalho-Pereira, Joana; Franco-Duarte, Ricardo; Sampaio, PaulaIntroduction Multiplex quantitative polymerase chain reaction (qPCR) methods for the detection of Aspergillus spp. based only on SYBR Green and melting curve analysis of PCR products are difficult to develop because most targets are located within ITS regions. The aim of this study was to adapt our previously developed methodology based on a multiplex PCR assay coupled with GeneScan analysis to provide a qPCR m...
Oral Candida albicans colonization in healthy individuals: prevalence, genotypi...
Gerós-Mesquita, Ângela; Carvalho-Pereira, Joana; Franco-Duarte, Ricardo; Alves, Armandino; Gerós, H.; Pais, Célia; Sampaio, PaulaIn this study, 181 healthy individuals, including 29 couples, were analysed regarding oral yeast colonization using a culture-based approach. Results showed that 39% of the individuals were yeast carriers, 89% being colonized with Candida albicans, 5% with C. guilliermondi, 3% with C. lusitaniae and 3% with C. parapsilosis. Sixty-two percent of the couples had at least one member colonized. Colonization and CFU...
Multiplex PCR based strategy for detection of fungal pathogen DNA in patients w...
Carvalho-Pereira, Joana; Fernandes, Filipa; Araújo, Ricardo; Springer, Jan; Loeffler, Juergen; Buitrago, María José; Pais, Célia; Sampaio, PaulaA new and easy polymerase chain reaction (PCR) multiplex strategy, for the identification of the most common fungal species involved in invasive fungal infections (IFI) was developed in this work. Two panels with species-specific markers were designed, the <i>Candida Panel</i> for the identification of <i>Candida</i> species, and the <i>Filamentous Fungi Panel</i> for the identification of <i>Aspergillus</i> sp...
Participation of Candida albicans transcription factor RLM1 in cell wall biogen...
Delgado-Silva, Yolanda; Vaz, Catarina; Carvalho-Pereira, Joana; Carneiro, Catarina; Nogueira, Eugénia; Correia, Alexandra; Carreto, Laura; Silva, SóniaCandida albicans cell wall is important for growth and interaction with the environment. RLM1 is one of the putative transcription factors involved in the cell wall integrity pathway, which plays an important role in the maintenance of the cell wall integrity. In this work we investigated the involvement of RLM1 in the cell wall biogenesis and in virulence. Newly constructed C. albicans Δ/Δrlm1 mutants showed t...