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An Efficient Agrobacterium-Mediated Genetic Transformation Method for Solanum b...

Cordeiro, Daniela; Alves, Ana; Ferraz, Ricardo; Casimiro, Bruno; Canhoto, Jorge; Correia, Sandra

Somatic embryogenesis in Solanum betaceum (tamarillo) has proven to be an effective model system for studying morphogenesis, since optimized plant regeneration protocols are available, and embryogenic competent cell lines can be induced from different explants. Nevertheless, an efficient genetic transformation system for embryogenic callus (EC) has not yet been implemented for this species. Here, an optimized f...


Enhancing the Production of Hydrolytic Enzymes in Elicited Tamarillo (Solanum b...

Casimiro, Bruno; Mota, Inês; Veríssimo, Paula; Canhoto, Jorge M. P. L.; Correia, Sandra

Plant cell suspension cultures are widely used as a tool for analyzing cellular and molecular processes, metabolite synthesis, and differentiation, bypassing the structural complexity of plants. Within the range of approaches used to increase the production of metabolites by plant cells, one of the most recurrent is applying elicitors capable of stimulating metabolic pathways related to defense mechanisms. Prev...


NEP-TC a rRNA Methyltransferase Involved on Somatic Embryogenesis of Tamarillo ...

Correia, Sandra; Alhinho, Ana T.; Casimiro, Bruno; Miguel, Célia M; Oliveira, Margarida; Veríssimo, Paula; Canhoto, Jorge

Somatic embryogenesis (SE) is an important biotechnological tool for large-scale clonal propagation and for embryogenesis research. Moreover, genetic transformation and cryopreservation procedures in many species rely on efficient SE protocols. We have been studying different aspects related to SE induction and somatic embryo development in tamarillo (Solanum betaceum Cav.), a small tree from the Solanaceae fam...


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