77 documents found, page 1 of 8
Interleukin-8 and Interleukin-6 are biomarkers of poor prognosis in Esophageal ...
Pastrez, Paula Roberta Aguiar; Barbosa, Ana Margarida; Mariano, Vânia Sammartino; Causin, Rhafaela Lima; Castro, António G.; Torrado, EgídioEsophageal squamous cell carcinoma (ESCC) is a common type of cancer characterized by fast progression and high mortality rates, which generally implies a poor prognosis at time of diagnosis. Intricate interaction networks of cytokines produced by resident and inflammatory cells in the tumor microenvironment play crucial roles in ESCC development and metastasis, thus influencing therapy efficiency. As such, cyt...
Highly tailorable gellan gum nanoparticles as a platform for the development of...
Rodrigues, Daniel Barreira; Moreira, Helena R.; Cerqueira, Mariana T.; Marques, A. P.; Castro, António G.; Reis, R. L.; Pirraco, Rogério P.Background: T cell priming has been shown to be a powerful immunotherapeutic approach for cancer treatment in terms of efcacy and relatively weak side efects. Systems that optimize the stimulation of T cells to improve therapeutic efcacy are therefore in constant demand. A way to achieve this is through artifcial antigen presenting cells that are complexes between vehicles and key molecules that target relevant...
Increased CD3+, CD8+, or FoxP3+ T lymphocyte infiltrations are associated with ...
Barbosa, Ana Margarida Martins; Martinho, Olga; Cardoso, Rosete Maria Amorim Novais Nogueira; Campos, Juliana; Lobo, Liliana; Pinto, HenriqueTumor-infiltrating lymphocytes include heterogeneous populations of T lymphocytes that play crucial roles in the tumor immune response; importantly, their presence in the tumor tissue may predict clinical outcomes. Therefore, we herein studied the prognostic significance of the presence and location of CD3<sup>+</sup>, CD8<sup>+</sup>, and FoxP3<sup>+</sup> T lymphocytes in colorectal cancer samples. In the int...
Towards the development of a female animal model of T1DM using hyaluronic acid ...
Zamboni, Fernanda; Cengiz, Ibrahim F.; Barbosa, Ana M.; Castro, António G.; Reis, R. L.; Oliveira, Joaquim M.; Collins, Maurice N.Female mice (Black 6 strain) (C57BL/6) aged 6 weeks were subject to low dose streptozotocin (STZ) treatment for five consecutive days to mimic type 1 diabetes mellitus (T1DM) with insulitis. At two weeks after STZ injections, evaluation of the elevated glucose levels was used to confirm diabetes. The diabetic mice were then subject to the transplantation of pancreatic β-cells (MIN-6 line). Four groups of mice w...
Predictors and outcomes of disseminated tuberculosis in an intermediate burden ...
Meira, L.; Chaves, Catarina; Araújo, D.; Almeida, L.; Boaventura, R.; Ramos, A.; Carvalho, T.; Osório, Nuno S.; Castro, António G.Setting: University-affiliated hospital located in Porto, North Portugal, an area with a low to intermediate incidence of tuberculosis (TB). Objective: To identify predictors and outcomes of disseminated TB (dTB). Design: A cohort of patients diagnosed with TB between 2007 and 2013 was retrospectively analysed. Patients with dTB criteria were characterized and compared to single organ TB cases. Factors independ...
Antimicrobial activity of Mycobacteriophage D29 Lysin B during Mycobacterium ul...
Fraga, Alexandra G.; Branquinho, Gabriela Alexandra Trigo; Murthy, Ramya K.; Akhtar, Shamim; Hebbur, Madhavi; Pacheco, Ana Rita; Dominguez, JuanBuruli Ulcer (BU) is a necrotizing skin disease caused by Mycobacterium ulcerans. Although the current antibiotic treatment for BU is effective, daily administrations for a prolonged period of time, combined with potential risk of severe side effects, negatively impact on patient adherence. In that sense, we tested the efficacy of an alternative strategy based on Lysin B (LysB), a phage encoded lipolytic enzyme...
The impact of IL-10 dynamic modulation on host immune response against visceral...
Mesquita, Inês Morais; Ferreira, Carolina; Barbosa, Ana Margarida; Ferreira, Catarina Machado; Moreira, Diana; Carvalho, Agostinho; Cunha, CristinaLeishmaniasis is a vector-borne disease caused by protozoan parasites from the genus Leishmania. The most severe form of disease is visceral leishmaniasis (VL), which is fatal if left untreated. It has been demonstrated that interleukin (IL)-10, is associated with disease progression and susceptibility. In this work, we took advantage of a transgenic mouse model that expresses high levels of IL-10 upon zinc sul...
The Dynamics of Interleukin-10-Afforded Protection during Dextran Sulfate Sodiu...
Cardoso, Ana; Castro, António G.; Martins, Ana Catarina; Carriche, Guilhermina M.; Murigneux, Valentine; Castro, Isabel; Cumano, Ana; Vieira, PauloInflammatory bowel disease encompasses a group of chronic-inflammatory conditions of the colon and small intestine. These conditions are characterized by exacerbated inflammation of the organ that greatly affects the quality of life of patients. Molecular mechanisms counteracting this hyperinflammatory status of the gut offer strategies for therapeutic intervention. Among these regulatory molecules is the anti-...
L-Threonine Supplementation During Colitis Onset Delays Disease Recovery
Gaifem, Joana; Gonçalves, Luís G.; Dinis-Oliveira, Ricardo J.; Cunha, Cristina; Carvalho, Agostinho; Torrado, EgídioDietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most ad...
A Nonribosomal Peptide Synthase Gene Driving Virulence in Mycobacterium tubercu...
Bhatt, Kiranmai; Machado, Henrique Pedro Pereira Silva Amaral; Osório, Nuno S.; Sousa, Jeremy; Cardoso, Filipa; Magalhães, Carlos André RodriguesNonribosomal peptide synthases produce short peptides in a manner that is distinct from classical mRNA-dependent ribosome-mediated translation. The Mycobacterium tuberculosis genome harbors a nonribosomal peptide synthase gene, nrp, which is part of a gene cluster proposed to be involved in the biosynthesis of isonitrile lipopeptides. Orthologous clusters are found in other slow-growing pathogenic mycobacteria ...