61 documents found, page 1 of 7
The fungicide cymoxanil impairs respiration in Saccharomyces cerevisiae via cyt...
Mendes, Filipa Diana Silva; Santos-Pereira, Cátia; Vieira, Tatiana F.; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase...
Macroinvertebrate communities reveal regional asymmetries in riverine ecosystem...
Santos, Joana Isabel; Silva, Carlos; Gonçalves, Fernando J. M.; Pereira, Joana Luísa; Castro, Bruno B.Climate change has been increasing the frequency and severity of drought periods. There is a need to understand how this water scarcity affects aquatic communities. The main aim of this work was to compare the macroinvertebrate communities and their bioindicator value (ecological status sensu the Water Framework Directive) in three Regions [northeast (N), central (C) and south (S)] of Portugal along a water ava...
Novel yeast-based biosensor for environmental monitoring of tebuconazole
Mendes, Filipa; Miranda, Eduarda; Amaral, Leslie Ann Silva; Carvalho, Carla; Castro, Bruno B.; Sousa, Maria João; Chaves, Susana Alexandra RodriguesAbstract: Due to increasing demand for high and stable crop production, human populations are highly dependent on pesticide use for growing and storing food. Environmental monitoring of these agrochemicals is therefore of utmost importance, because of their collateral effects on ecosystem and human health. Even though most current-use analytical methods achieve low detection limits, they require procedures that...
Chronic exposure to the synthetic cathinone 3,4-methylenedioxypyrovalerone (MDP...
Pérez-Pereira, Ariana; Costa, Pedro A.; Castro, Bruno B.; Carrola, João S.; Gonçalves, Virgínia M. F.; Ribeiro, Ana R. L.; Fernandes, CarlaBackground: The abuse of synthetic cathinones (SC) is popular among young consumers for recreational purposes and has increased dramatically in the last years. Consequently, SC have been found in the aquatic environment at low concentrations [1]. Since SC are designed to change nervous system function, they may pose unpredictable harmful effects on non-target organisms, such as aquatic invertebrates [2]. Many S...
Toxicity of the 3,4-methylenedioxymethamphetamine and its enantiomers to Daphni...
Costa, Ana Rita; Gonçalves, Virgínia M. F.; Castro, Bruno B.; Carrola, João Soares; Langa, Ivan; Pereira, Ariana; Carvalho, Ana RitaMDMA (3,4-methylenedioxymethamphetamine) is a chiral psychoactive recreational drug sold in illicit markets as racemate. Studies on the impact of MDMA on aquatic organisms are scarce. While enantioselectivity in toxicity in animals and humans has been reported, none is reported on aquatic organisms. This study aimed to investigate the ecotoxicological effects of MDMA and its enantiomers in <i>Daphnia magna</i>....
Cymoxanil inhibits respiration through inhibition of mitochondrial complex IV
Mendes, Filipa; Vieira, Tatiana F.; Pereira, Cátia Sofia Santos; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil is a synthetic acetamide fungicide, used against oomycetes. It was first introduced in 1977 and can be used against downy mildew diseases induced by Plasmopara viticola in grapevine cultures and late blight diseases caused by Phytophthora infestans, in tomatoes and potatoes cultures. This fungicide is used in mixed formulations and its higher solubility enables a relatively widespread occurrence in to...
Enantioselectivity in the ecotoxicity of amphetamine using daphnia magna as the...
Ribeiro, Cláudia; Gomes, Cristiano; Pérez-Pereira, Ariana; Carrola, João Soares; Tiritan, Maria Elizabeth; Langa, Ivan; Couto, CristinaAmphetamine (AMP) is a chiral psychoactive substance that exhibits enantioselectivity in its pharmacological properties. It has been detected in wastewaters and surface waters and can occur as enantiomeric mixtures, but little is known about its environmental risk and potential enantioselective toxicity to aquatic organisms. Our study aimed to target enantioselectivity in AMP toxicity to the freshwater inverteb...
Macroinvertebrate community structure and ecological status in Portuguese strea...
Santos, Joana Isabel; Silva, Carlos; Goncalves, Fernando J. M.; Pereira, Joana Luísa; Castro, Bruno B.Climatic and hydrological variation is of utmost importance in regions of the globe facing water scarcity and river intermittency (e.g. areas under Mediterranean influence). The main aim of this study was to compare the macroinvertebrate community structure and its bioindicator value (i.e. waterbody ecological status) in streams from three Portuguese regions (Regions C, N and S), representing distinct climatic ...
Enantioselective ecotoxicity of venlafaxine in aquatic organisms: Daphnia and z...
Ribeiro, Ondina; Félix, Luís; Ribeiro, Cláudia; Castro, Bruno B.; Tiritan, Maria Elizabeth; Monteiro, Sandra Mariza; Carrola, João SoaresVenlafaxine is a chiral antidepressant detected in aquatic compartments. It was recently included in the 3rd Watch List from the European Union. The present study aimed to investigate venlafaxine toxicity effects, targeting possible enantioselective effects, using two aquatic organisms, daphnia (Daphnia magna) and zebrafish (Danio rerio). Specimens were exposed to both racemate, (R,S)-venlafaxine (VEN), and to ...
Antiparasitic potential of agrochemical fungicides on a non-target aquatic mode...
Machado, Cláudia; Cuco, Ana P.; Cássio, Fernanda; Wolinska, Justyna; Castro, Bruno B.Pesticides are a major anthropogenic threat to the biodiversity of freshwater ecosystems, having the potential to affect non-target aquatic organisms and disrupt the processes in which they intervene. Important knowledge gaps have been recognised concerning the ecological effects of synthetic fungicides on non-target symbiotic aquatic fungi and the ecological processes where they intervene. The goal of this wor...