8 documents found, page 1 of 1
Epidemiology and Burden of Ventilator-Associated Pneumonia among Adult Intensiv...
Mergulhão, P; Pereira, JG; Fernandes, AV; Krystopchuk, A; Ribeiro, J; Miranda, D; Castro, H; Eira, C; Morais, J; Lameirão, C; Gomes, S; Leal, DVentilator-associated pneumonia (VAP) is a prevailing nosocomial infection in critically ill patients requiring invasive mechanical ventilation (iMV). The impact of VAP is profound, adversely affecting patient outcomes and placing a significant burden on healthcare resources. This study assessed for the first time the contemporary VAP epidemiology in Portugal and its burden on the healthcare system and clinical...
Epidemiology and Burden of Ventilator-Associated Pneumonia among Adult Intensiv...
Mergulhão, P; Pereira, JG; Fernandes, AV; Krystopchuk, A; Ribeiro, JM; Miranda, D; Castro, H; Eira, C; Morais, J; Lameirão, C; Gomes, S; Leal, DVentilator-associated pneumonia (VAP) is a prevailing nosocomial infection in critically ill patients requiring invasive mechanical ventilation (iMV). The impact of VAP is profound, adversely affecting patient outcomes and placing a significant burden on healthcare resources. This study assessed for the first time the contemporary VAP epidemiology in Portugal and its burden on the healthcare system and clinical...
Epigallocathechin-O-3-Gallate Inhibits Trypanothione Reductase of Leishmania in...
Inacio, JDF; Fonseca, MS; Limaverde-Sousa, G; Tomás, AM; Castro, H; Almeida-Amaral, EELeishmania infantum is a protozoan parasite that causes a vector borne infectious disease in humans known as visceral leishmaniasis (VL). This pathology, also caused by L. donovani, presently impacts the health of 500,000 people worldwide, and is treated with outdated anti-parasitic drugs that suffer from poor treatment regimens, severe side effects, high cost and/or emergence of resistant parasites. In previou...
Synthesis and evaluation of novel chromanone and quinolinone analogues of unifl...
Castro, H; Cruz, T; Amaral, PA; Cardoso, PS; Alsaffar, A; Farrell, P; Tomás, AM; Barlow, JWWithin this work, we describe the design and synthesis of a range of novel chromanones and quinolinones, based on natural products reported to possess anti-leishmanial action. The core heterocycles were obtained either via classical or ionic liquid mediated Kabbe condensation in the case of chromanones, or aqueous Sonogashira based alkynylation followed by acid-catalysed cyclisation in the case of quinolinones....
Chaperone activation and client binding of a 2-cysteine peroxiredoxin
Teixeira, F; Tse, E; Castro, H; Makepeace, KAT; Meinen, BA; Borchers, CH; Poole, LB; Bardwell, JC; Tomás, AM; Southworth, DR; Jakob, UMany 2-Cys-peroxiredoxins (2-Cys-Prxs) are dual-function proteins, either acting as peroxidases under non-stress conditions or as chaperones during stress. The mechanism by which 2-Cys-Prxs switch functions remains to be defined. Our work focuses on Leishmania infantum mitochondrial 2-Cys-Prx, whose reduced, decameric subpopulation adopts chaperone function during heat shock, an activity that facilitates the tr...
Development of an automated image analysis protocol for quantification of intra...
Gomes-Alves, AG; Maia, AF; Cruz, T; Castro, H; Tomás, AMLeishmania parasites cause a set of neglected tropical diseases with considerable public health impact, the leishmaniases, which are often fatal if left untreated. Since current treatments for the leishmaniases exhibit high toxicity, low efficacy and prohibitive prices, many laboratories throughout the world are engaged in research for the discovery of novel chemotherapeutics. This entails the necessity of scre...
Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in Leishma...
Teixeira, F; Castro, H; Cruz, T; Tse, E; Koldewey, P; Southworth, DR; Tomás, AM; Jakob, UCytosolic eukaryotic 2-Cys-peroxiredoxins have been widely reported to act as dual-function proteins, either detoxifying reactive oxygen species or acting as chaperones to prevent protein aggregation. Several stimuli, including peroxide-mediated sulfinic acid formation at the active site cysteine, have been proposed to trigger the chaperone activity. However, the mechanism underlying this activation and the ext...
Mucocutaneous pathology in HIV infection.
Lamarão, P; Eliseu, T; Castro, H; Maltez, F; Machado, J; Morgado, A; Proença, RSeveral infectious, neoplastic or inflammatory skin diseases, may be early manifestations of HIV infection. We reviewed the clinical data of 226 HIV seropositive male patients with cutaneous disease, from a total of 337 inpatients at the Infectious Diseases Unit--Department of Medicine 1--Curry Cabral Hospital, from 1985 to 1991. Some considerations are made about the most common dermatological disorders: oral ...