16 documents found, page 1 of 2
Protective effect of ions against cell death induced by acid stress in Saccharo...
Sant’Ana, Gilzeane dos Santos; Paes, Lisvane da Silva; Paiva, Argentino F. Vieira; Fietto, Luciano Gomes; Totola, Antônio HelvécioSaccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under t...
New Lager Brewery Strains obtained by crossing techniques using cachaça (Brazil...
Queiroz, Marisa Vieira de; Figueiredo, Bruna Inez Carvalho; Saraiva, Margarete Alice Fontes; Pimenta, Paloma Patrick de SouzaThe development of hybrids has been an effective approach to generate novel yeast strains with optimal technological profile for use in beer production. This study describes the generation of a new yeast strain for lager beer production by direct mating between two Saccharomyces cerevisiae strains isolated from cachaça distilleries: one that was strongly flocculent, and the other with higher production of aceta...
Investigating acid stress response in different saccharomyces strains.
Brandão, Rogélio Lopes; Rosa, Júlio César Câmara; Nicoli, Jacques Robert; Almeida, Marcos Vinicius Simi; Carmo, Ana Paula do; Queiros, Heloa TeixeiraYeast cells need to respond to a variety of stresses found in such different conditions as gastrointestinal tract after probiotic ingestion or fermentation vat during ethanol production. In the present study, H+ neutralisation capacity, membrane fatty acid composition, H+-ATPase activity, and cytosolic Ca2+ concentration were evaluated in yeast cells used for probiotic (Saccharomyces boulardii) and laboratory (...
Purification and characterization of an extracellular trypsin-like protease of ...
Barata, Ricardo Andrade; Andrade, Milton Hércules Guerra de; Rodrigues, Roberta Dias; Castro, Ieso de MirandaAn alkaline serineprotease, capable of hydrolyzing Nu-benzoyl-DL arginine p-nitroanilide, was secreted by Fusurium oxysporum var. hi grown in the presence of gelatin as the sole nitrogen and carbon source. The protease was purified 65-fold to electrophoretic homogenity from the culture supernatant in a three-step procedure comprising QSepharose chromatography, aMnity chromatography, and FPLC on a MonoQ column. ...
Genetic ancestry is associated with systolic blood pressure and glucose in Braz...
Queiroz, Erica Maria de; Barbosa, Priscila Oliveira; Candido, Ana Paula; Castro, Ieso de Miranda; Coelho, George Luiz Lins Machado; Leite, Tailce MouraBackground: Studies in admixed populations show that the prevalence of obesity and related diseases, such as type 2 diabetes and hypertension, may vary by ethnic group. The aim of this study was to investigate the relationship of genetic ancestry with phenotypes associated with obesity in a sample of school children and adolescents from Ouro Preto, Minas Gerais. Methods: We used data from genetic ancestry of 18...
Glucose induced activation of the plasma membrane ATPase in Fusarium oxysporum.
Brandão, Rogélio Lopes; Castro, Ieso de Miranda; Passos, Jomar Becher dos; Nicoli, Jacques Robert; Thevelein, Johan MariaAddition of glucose and other sugars to derepressed cells of the fungus Fusarium oxysporum var. Zini triggered activation of the plasma membrane H+-ATPase within 5 min. Glucose was the best activator while galactose and lactose had a lesser effect. The activation was not prevented by previous addition of cycloheximide and it was fully reversible when the glucose was removed. The activation process in uiuo also ...
Variable flocculation profiles of yeast strains isolated from cachaça distiller...
Alvarez, Florencia; Correa, Lygia Fátima da Mata; Araújo, Thalita Macedo; Mota, Bruno Eduardo Fernandes; Conceição, Luís Eduardo Fernandes Rodrigues daIn cachaça production, the use of yeast cells as starters with predictable flocculation behavior facilitates the cell recovery at the end of each fermentation cycle. Therefore, the aim of this work was to explain the behavior of cachaça yeast strains in fermentation vats containing sugarcane through the determination of biochemical and molecular parameters associated with flocculation phenotypes. By analyzing t...
Effect of the trehalose levels on the screening of yeast as probiotic by in viv...
Martins, Flaviano dos Santos; Castro, Ieso de Miranda; Rosa, Carlos Augusto; Nicoli, Jacques Robert; Neves, Maria JoséProbiotics are viable defined microorganisms (bacteria or yeasts) that exert a beneficial effect on the health of the host when ingested in adequate amounts. Screening for such biotherapeutic agents is commonly performed by in vitro assays simulating gastrointestinal environment to determine the ability to survive in the digestive tract. In the present study, the possibility of extrapolation of data obtained in...
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effect...
Dutra, Alexandre Augusto de Assis; Sousa, Lucas Oliveira; Resende, Rodrigo Ribeiro; Brandão, Rogélio Lopes; Kalapothakis, EvanguedesHere, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LT...
Protective effect of ions against cell death induced by acid stress in Saccharo...
Sant'Ana, Gilzeane dos Santos; Paes, Lisvane da Silva; Paiva, Argentino F. Vieira; Fietto, Luciano Gomes; Tótola, Antônio HelvécioSaccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under t...