56 documents found, page 1 of 6
Application of antisense therapy to control Nakaseomyces glabratus infections
Serra, Patrícia; Gonçalves, Bruna; Henriques, Mariana; Castro, Joana; Araújo, Daniela; Silva, Sónia CarinaCanclicla infections affect billions of people worlclwicle, with high morbidity ancl mortality rates (40-60CX,) [l] ancl require long hospital stays ancl increase healthcare costs, which places a significant socio-economic burclen on the economy [2]. Canclicla glabrata (now reclassified as Nakaseomyces glabratus) poses a significant clinical challenge, clue to its increasing levels of resistance to conventional...
Advances in whole genome sequencing for foodborne pathogens: implications for c...
Gomes, Emílio; Araújo, Daniela; Nogueira, Teresa; Oliveira, Ricardo; Silva, Sónia; Oliveira, Lorena V. N.; Azevedo, Nuno F.; Almeida, CarinaFoodborne outbreaks affecting millions of people worldwide are a significant and growing global health threat, exacerbated by the emergence of new and increasingly virulent foodborne pathogens. Traditional methods of detecting these outbreaks, including culture-based techniques, serotyping and molecular methods such as real time PCR, are still widely used. However, these approaches often lack the precision andr...
The effects of vessel traffic on the behavior patterns of common dolphins in th...
Silva, M. M.; Alves Jesus, Nádia; Castro, Joana; Luís, Ana RitaThe impact of vessels on dolphin populations has been extensively studied worldwide. The common dolphin, Delphinus delphis, has been observed in the Tagus estuary for the past two centuries, and during the last several years, these sightings seem to have increased. This area has high levels of maritime traffic throughout the year, both commercial and recreational. To understand the possible effects of vessel tr...
Evaluation of simultaneous growth of Escherichia coli O157:H7, Salmonella spp.,...
Sousa, José M.; Barbosa, Ana; Araújo, Daniela Eira; Castro, Joana; Azevedo, Nuno Filipe; Cerqueira, Laura; Almeida, Carina Manuela FernandesSeveral multiplex approaches for the simultaneous detection of pathogens in food have been developed in recent years, but the use of a single enrichment medium remains a problem. In this study, six enrichment broths (five non-selective media, tryptic soy broth (TSB), brain heart infusion broth (BHI), buffered peptone water (BPW), universal pre-enrichment broth (UPB), no. 17 broth, and a selective, Salmonella Es...
Avaliação do potencial de sobrenadantes livres de células (SLC) contra agentes ...
Ferreira, Sara; Gomes, Emílio; Araújo, Daniela; Azevedo, Nuno F.; Almeida, Carina Manuela Fernandes; Castro, JoanaOs agentes patogénicos de origem alimentar são um problema recorrente de saúde pública, causando surtos frequentes que podem ter consequências drásticas. De modo a combater estes microrganismos, diversas medidas são habitualmente aplicadas, nomeadamente o uso de antibióticos e desinfetantes. No entanto, o uso excessivo destas substâncias pode levar ao desenvolvimento de resistência ou tolerância por parte dos m...
Developing translucent emulsions using sustainable NADES-based extracts
Freitas, David; Rocha, Diana; Santos, Joana; Noro, Jennifer Martins; Tavares, Tânia D.; Teixeira, Marta O.; Araújo, Daniela; Castro, JoanaIn this work, we explored natural deep eutectic solvents (NADES) for developing translucent NADES-in-oil emulsions (TEs) for cosmetic applications. NADES, composed of lactic acid and glycerol (1:1 and 1:4 ratios), were selected for their natural origin, non-toxicity, and compatibility with living organisms. These green solvents facilitated the extraction of bioactive compounds from Lavandula angustifolia, Corym...
Multidrug-resistant Klebsiella pneumoniae and Klebsiella variicola isolated fro...
Castro, Joana; Araújo, Daniela Eira; Oliveira, Hugo Alexandre Mendes; Fernandes, Liliana Sousa; Oliveira, Ricardo; Brinks, Erick; Cho, Gyu-SungThe emergence of antibiotic-resistant Klebsiella spp. has become a huge problem in clinical settings. In this study, we aimed to characterize the molecular and phenotypic features of 21 MDR Klebsiella isolates (n =19 of K. pneumoniae, and n =2 of K. variicola) isolated from patients admitted to two central hospitals in northern Portugal by using whole-genome sequencing (WGS), followed by an in vitro assessment ...
Emerging approaches for mitigating biofilm-formation-associated infections in f...
Araújo, Daniela Eira; Silva, Ana Rita; Fernandes, Rúben Rodrigues; Serra, Patrícia; Barros, Maria Margarida; Campos, Ana Maria; Oliveira, RicardoThe importance of addressing the problem of biofilms in farm, wild, and companion animals lies in their pervasive impact on animal health and welfare. Biofilms, as resilient communities of microorganisms, pose a persistent challenge in causing infections and complicating treatment strategies. Recognizing and understanding the importance of mitigating biofilm formation is critical to ensuring the welfare of anim...
Valores no ensino e na aprendizagem da matemática: Os aspetos mais valorizados ...
Silvestre, Ana Isabel; Jacinto, Hélia; Serrazina, Lurdes; Amado, Nélia; Carreira, Susana; Santos, Elvira; Tomás Ferreira, Rosa; Martins, CristinaEste artigo apresenta o primeiro estudo alargado realizado em Portugal onde se identificam e discutem aspetos que os professores de matemática mais valorizam no ensino e na aprendizagem desta disciplina. O estudo seguiu uma metodologia qualitativa e recorreu a uma análise de conteúdo para categorizar as respostas dos participantes, obtidas através de um questionário eletrónico. Foram inquiridos 113 professores ...
Valores no ensino e na aprendizagem da matemática: os aspetos mais valorizados ...
Silvestre, Ana Isabel; Jacinto, Hélia; Serrazina, Lurdes; Amado, Nélia; Carreira, Susana; Santos, Elvira Maria; Ferreira, Rosa Tomás; Martins, CristinaEste artigo apresenta o primeiro estudo alargado realizado em Portugal onde se identificam e discutem aspetos que os professores de matemática mais valorizam no ensino e na aprendizagem desta disciplina. O estudo seguiu uma metodologia qualitativa e recorreu a uma análise de conteúdo para categorizar as respostas dos participantes, obtidas através de um questionário eletrónico. Foram inquiridos 113 professores ...