6 documents found, page 1 of 1
Detection and Identification of extra virgin olive oil adulteration by GC-MS co...
Yang, Yang; Ferro, Miguel Duarte; Cavaco, Isabel Maria Palma Antunes; Liang, YizengIn this study, an analytical method for the detection and identification of extra virgin olive oil adulteration with four types of oils (corn, peanut, rapeseed, and sunflower oils) was proposed. The variables under evaluation included 22 fatty acids and 6 other significant parameters (the ratio of linoleic/linolenic acid, oleic/linoleic acid, total saturated fatty acids (SFAs), polyunsaturated fatty acids (PUFA...
Binding of oxovanadium(IV) complexes to blood serum albumins
Cobbina, Enoch; Mehtab, Sameena; Correia, Isabel; Gonçalves, Gisela; Tomaz, Isabel; Cavaco, Isabel Maria Palma Antunes; Jakusch, Tamás; Eneyedi, EvaIn this work the binding of VIVO2+ and VIVO-complexes to serum albumins {human serum albumin (HSA), bovine serum albumin (BSA) and porcine serum albumin (PSA)} are studied using circular dichroism (CD), electron paramagnetic resonance (EPR) and visible absorption spectroscopy. The results confirm previous findings that VIVO2+ occupies at least two types of binding sites on albumin: ‘the strong vanadium binding ...
Antioxidant, DNA cleavage, and cellular effects of silibinin and a new oxovanad...
Naso, Luciana G.; Ferrer, Evelina G.; Butenko, Nataliya; Cavaco, Isabel Maria Palma Antunes; Lezama, Luis; Rojo, Teófilo; Etcheverry, Susana B.A new complex of the oxovanadium(IV) cation with the flavolignan silibinin has been synthesized and characterized. Vanadium compounds show interesting biological and pharmacological properties and some of them display antitumoral actions. Flavonoids are part of a larger group of antioxidant compounds called polyphenols which may inhibit the proliferation and growth of cancer cells. The antioxidant and antitumor...
Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colo...
Di Virgilio, Ana L.; Rivadeneira, Josefina; Muglia, Cecilia I.; Reigosa, Miguel A.; Butenko, Nataliya; Cavaco, Isabel Maria Palma AntunesThe complex of vanadyl(IV) cation with oxodiacetate, VO(oda) caused an inhibitory effect on the proliferation of the human colon adenocarcinoma cell line Caco-2 in the range of 25–100 lM (P\0.001). This inhibition was partially reversed by scavengers of free radicals. The difference in cell proliferation in the presence and the absence of scavengers was statistically significant in the range of 50–100 lM (P\0.0...
DNA cleavage activity of VIVO(acac)2 and derivatives
Butenko, Nataliya; Tomaz, Ana Isabel; Nouri, Ofelia; Escribano, Esther; Moreno, Virtudes; Gama, Sofia; Ribeiro, Vera; Telo, João PauloThe DNA cleavage activity of several b-diketonate vanadyl complexes is examined. Vanadyl acetylacetonate,VIVO(acac)2, 1, shows a remarkable activity in degrading plasmid DNA in the absence of any activating agents, air and photoirradiation. The cleaving activity of several related complexes VIVO(hd)2(2, Hhd = 3,5-heptanedione), VIVO(acac-NH2)2 (3, Hacac-NH2 = acetoacetamide) and VIVO(acac-NMe2)2(4, Hacac-NMe2 =...
Electrochemical DNA sensor for detection of single nucleotide polymorphisms
Marques, L. P. J.; Cavaco, Isabel Maria Palma Antunes; Pinheiro, J. P.; Ribeiro, Vera; Ferreira, GuilhermeIn recent years there has been an increased interest in using biosensors for the recognition and monitoring of molecule interactions. DNA sensors and gene chips are particularly relevant for directly applying the information gathered from the genome projects. In this work electrochemical techniques are used to develop methodologies to detect DNA polymorphisms in human genes using cytochrome P450 3A4 (CYP3A4) as...