12 documents found, page 1 of 2
Atividade hemaglutinante em sementes de dioclea rostrata BENTH
Grangeiro, T. B.; Oliveira, I. T. A. de; Moreira, R. A.; Cavada, B. S.Seeds of Dioc/ea rostrata Benth. Have a lectin which is best extracted with Na-acetate buffer at pH 4.0 and that is selectively adsorbed in a Sephadex G-50 matrix, yielding a protein fraction of high hemagg lutinating titre against rabbit erythrocytes. I t also agglu tinates sheep, and human cells inespecifically. Its hemagglutinating activity is inhibited by glucose fructose, mannose and O-methyl-D-mannopyrano...
Estudos preliminares de uma lecflna de sementes de parkia platycefhala BENTH.
Grangeiro, T. B.; Oliveira, J. T. A. de; Moreira, R. A.; Cavada, B. S.Seecis af Parkia platycephala Benth. have a lectin which is best extracted at pH 4,0 and partially purified using a Séphadex G-50 column as an affinity adsorbent. The lectin .agglutinates rabbit erythrocytes but not those of cow, sheep, chicken or human D-glucose, D-mannose, D-fructose and derivatives act as inhibitors of the lectin with o-methyl- glucopyranoside and O-methyl-mannopyranoside being the most pote...
Crotacetin, a novel snake venom c-type lectin, is homolog of convulxin
Rádis-Baptista, Gandhi; Moreno, F. B. M. B.; Nogueira, L. L.; Martins, A. M. C.; Tomaya, D. O; Tomaya, M. H; Azevedo Júnior, W. F.; Cavada, B. S.Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant similarity in their primary structures with C-type lectins of other animals, and also present a conserved carbohydrate recognition domain (CRD). A very well studied sv C-type lectin is the heterodimeric toxin, convulxin (CVX), from the venoms of South American rattlesnakes...
Crotacetin, a novel snake venom C-type lectin, is homolog of convulxin
Rádis-baptista, G.; Moreno, F. B. M. B.; Nogueira, L. L.; Martins, A. M. C.; Toyama, D. O.; Toyama, M. H. [UNESP]; Azevedo Jr, W. F. [UNESP]Made available in DSpace on 2021-07-14T10:50:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12. Added 1 bitstream(s) on 2021-07-14T11:39:11Z : No. of bitstreams: 1 S1678-91992005000400013.pdf: 827726 bytes, checksum: 65adf78968e48f1e78beea14818e20a7 (MD5); Snake venom (sv) C-type lectins encompass a group of hemorrhagic toxins, which are able to interfere with hemostasis. They share significant simil...
Purification and primary structure determination of a galactose-specific lectin...
Silva, Helton Colares; Nagano, C. S.; Souza, Luis A.G.; Nascimento, K. S.; Isídro, Renato; Delatorre, Plínio; Rocha, Bruno Anderson Matias daVatairea guianensis seeds, a typical plant from the Brazilian Amazon region that belongs to the Dalbergieae tribe, possess a lectin that was isolated by precipitation with solid ammonium sulfate followed by guar gum affinity chromatography. This lectin was named VGL. The V. guianensis lectin strongly agglutinated rabbit erythrocytes and was inhibited by d-galactose and d-galactose-derived sugars, especially N-a...
A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: Partia...
Alves, Ana Cecília; Vasconcelos, Mayron Alves de; Santiago, Mayara Quiroz; Pinto-Junior, Vanir Reis; Osterne, Vinicius José SilvaA novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) an...
Contribution of the carbohydrate-binding ability of Vatairea guianensis lectin ...
Marques, Gabriela Fernandes Oliveira; Osterne, Vinicius José Silva; Almeida, Livia M.; Oliveira, Messias Vital; Brizeno, Luiz André CavalcanteVatairea guianensis lectin (VGL), Dalbergiae tribe, is a N-acetyl-galactosamine (GalNAc)/Galactose (Gal) lectin previously purified and characterized. In this work, we report its structural features, obtained from bioinformatics tools, and its inflammatory effect, obtained from a rat paw edema model. The VGL model was obtained by homology with the lectin of Vatairea macrocarpa (VML) as template, and we used it ...
Fine specificities of two lectins from Cymbosema roseum seeds: A lectin specifi...
Dam, Tarun Kanti; Cavada, B. S.; Nagano, C. S.; Rocha, Bruno Anderson Matias da; Benevides, R. G.; Nascimento, K. S.; Sousa, Luiz A.G. deThe legume species of Cymbosema roseum of Diocleinae subtribe produce at least two different seed lectins. The present study demonstrates that C. roseum lectin I (CRL I) binds with high affinity to the "core" trimannoside of N-linked oligosaccharides. Cymbosema roseum lectin II (CRL II), on the other hand, binds with high affinity to the blood group H trisaccharide (Fuc1,2Gal1-4GlcNAc-). Thermodynamic and hemag...
Structural basis for both pro- and anti-inflammatory response induced by mannos...
Rocha, Bruno Anderson Matias da; Delatorre, Plínio; Oliveira, Taianá Maia; Benevides, R. G.; Pires, Alana de Freitas; Sousa, Albertina A.S.Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection and anti-inflammatory effects when by systemic injection. Protein sequencing was obtained by Tandem Mass Sp...
The complete genome sequence of Chromobacterium violaceum reveals remarkable an...
Vasconcelos, Ana Tereza; De-Almeida, Darcy Fontoura; Hungría, Mariangela; Guimarães, Cláudia Teixeira; Antônio, Regina VasconcellosChromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spre...