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Hydrochar as protein support: preservation of biomolecule properties with non-c...
Castro, Manuela Oliveira; Santiago, Mayara Queiroz de; Nascimento, Kyria Santiago do; Cavada, Benildo Sousa; Miguel, Emilio de CastroIn this work, the ConBr lectin was non-covalently immobilized onto hydrochar (HC). This carbonaceous material was produced by the hydrothermal carbonization of glucose and then put to interact with the lectin, aiming to immobilize the biomolecule via electrostatic interactions. Samples obtained after the interaction were characterized by CHNS elemental analysis, scanning electron microscopy and Fourier transfor...
Ultrasound processing to enhance drying of cashew apple bagasse puree: Influenc...
Fonteles, Thatyane Vidal; Leite, Ana Karoline Ferreira; Silva, Ana Raquel Araújo; Carneiro, Alessandra Pinheiro Góes; Miguel, Emilio de CastroThe present study has evaluated the effects of power ultrasound pre-treatment on air-drying and bioactive compounds of cashew apple bagasse. The sonication induced the disruption of cashew bagasse parenchyma, which resulted in lower resistance to water diffusion, less hysteresis, and increased rehydration rate. The processing did not affect the lignocellulose fibers or the clerenchyma cells. For sonicated sampl...
Seed structure in Canavalia brasiliensis Mart. ex Benth. (Leguminosae) and subc...
Miguel, Emilio de Castro; Miguel, Thaiz Batista Azevedo Rangel; Pireda, S.; Marques, Jonas de Brito Campolina; Cunha, Maura da; Cajazeiras, João BatistaLectins are proteins capable of specific and reversible recognition of carbohydrates without modifying them. Many studies have isolated these molecules from Leguminosae seeds but little attention was given to subcellular localization and biological function of these molecules. Therefore, this work aimed to describe Canavalia brasiliensis seed structure and the subcellular localization of ConBr. In addition, we ...
Dioclea violacea lectin ameliorates oxidative stress and renal dysfunction in a...
Freitas, Flavia P. S.; Porto, Marcella L.; Tranhago, Camilla P.; Piontkowski, Rogerio; Miguel, Emilio de Castro; Miguel, Thaiz Batista Azevedo RangelAcute kidney injury (AKI) is characterized by rapid and potentially reversible decline in renal function; however, the current management for AKI is nonspecific and associated with limited supportive care. Considering the need for more novel therapeutic approaches, we believe that lectins from Dioclea violacea (Dvl), based on their antiinflammatory properties, could be beneficial for the treatment of AKI induce...
Morphology, ultrastructure and immunocytochemistry of Hypnea cervicornis and Hy...
Miguel, Thaiz Batista Azevedo Rangel; Schmidt, Eder C.; Bouzon, Zenilda Laurita; Nascimento, Fernando E.P.; Cunha, Maura da; Fernandes, Saulo PiredaBased on their morphological and physiological features, red algae comprise a complex and variable group of multiple genera, including Hypnea. In particular, the genus Hypnea J.V. Lamouroux (Cystocloniaceae, Rhodophyta) consists of approximately 54 species, including Hypnea cervicornis and H. musciformis. Lectins were described for both species; however, the localization of these proteins is still unclear. Ther...
Purification, partial characterization and immobilization of a mannose-specific...
Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis AlmeidaLectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
Analysis of protein expression and a new prokaryotic expression system for goat...
Rádis-Baptista, Gandhi; Cajazeiras, João Batista; Melo, Luciana Magalhães; Albuquerque, Érica Larissa M. Souto de; Cavada, Benildo SousaLow purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant clones were selected by growth in amp...
Ion-exchange chromatography used to isolate a spermadhesin-related protein from...
Teixeira, Dárcio Ítalo Alves; Melo, Luciana Magalhães; Gadelha, Carlos Alberto de Almeida; Cunha, Rodrigo Maranguape Silva da; Bloch Júnior, CarlosMammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-excha...
Crotacetin, a Novel Snake Venom C-Type Lectin Homolog of Convulxin, Exhibits an...
Rádis-Baptista, Gandhi; Moreno, Frederico Bruno Mendes Batista; Nogueira, Lucas de Lima; Martins, Alice M. C.; Toyama, Daniela de OliveiraSnake venom (sv) C-type lectins encompass a group of hemorrhagic toxins that are capable of interfering with blood stasis. A very well-studied svC-type lectin is the heterodimeric toxin, convulxin (CVX), from the venom of South American rattlesnake Crotalus durissus terrificus. CVX is able to activate platelets and induce their aggregation by acting via p62/GPVI collagen receptor. By using polymerase chain reac...
An ab initio explanation of the activation and antagonism strength of an AMPA-s...
Martins, Ana Caroline Vieira; Lima Neto, Pedro de; Barroso Neto, Ito Liberato; Cavada, Benildo Sousa; Freire, Valder NogueiraWe report quantum biochemistry calculations focusing on the binding pocket of the glutamate receptor co-crystallized with agonists (full and partial) and a antagonist. The calculated electronic binding energy follows the order AMPA . glutamate. kainate . DNQX, which explains published experimental data on GluR2 activation and antagonism strength.