10 documents found, page 1 of 1
Alterations in mucosa branched N-glycans lead to dysbiosis and downregulation o...
Rodrigues, Cláudia S.; Gaifem, Joana; Pereira, Márcia S.; Alves, Maria Francisca; Silva, Mariana; Padrão, Nuno; Cavadas, BrunoThe perturbation of the symbiotic relationship between microbes and intestinal immune system contributes to gut inflammation and Inflammatory Bowel Disease (IBD) development. The host mucosa glycans (glycocalyx) creates a major biological interface between gut microorganisms and host immunity that remains ill-defined. Glycans are essential players in IBD immunopathogenesis, even years before disease onset. Howe...
Extracellular vesicles from pancreatic cancer stem cells lead an intratumor com...
Ruivo, Carolina F.; Bastos, Nuno; Adem, Barbara; Batista, Ines; Duraes, Cecilia; Melo, Carlos A.; Castaldo, Stephanie A.; Campos‐Laborie, FranciscoObjective: Intratumor heterogeneity drives cancer progression and therapy resistance. However, it has yet to be determined whether and how subpopulations of cancer cells interact and how this interaction affects the tumour. Design: We have studied the spontaneous flow of extracellular vesicles (EVs) between subpopulations of cancer cells: cancer stem cells (CSC) and non-stem cancer cells (NSCC). To determine th...
Hypoxia and Macrophages Act in Concert Towards a Beneficial Outcome in Colon Ca...
Martins, Flávia; Oliveira, Rosa; Cavadas, Bruno; Pinto, Filipe; Cardoso, Ana Patrícia; Castro, Flávia; Sousa, Bárbara; Pinto, Marta LaranjeiroIn colon cancer, the prognostic value of macrophages is controversial, and it is still unknown how hypoxia modulates macrophage-cancer cell crosstalk. To unravel this, co-cultures of human primary macrophages and colon cancer cells were performed at 20% and 1% O2, followed by characterization of both cellular components. Different colon cancer patient cohorts were analyzed for hypoxia and immune markers, and th...
Association of leukotriene A4 hydrolase with tuberculosis susceptibility using ...
Rito, Teresa; Ferreira, Joana; Cavadas, Bruno; Soares, Pedro; Oliveira, Olena; Richards, Martin B.; Duarte, Raquel; Pereira, Luísa; Correia-Neves, MLeukotriene A4 hydrolase (LTA4H) is a key enzyme in the eicosanoid pathway. <i>lta4h</i> locus polymorphisms have previously been linked to tuberculosis (TB) susceptibility and disease outcome in a Vietnamese dataset, but further studies suggested that those results were poorly reproducible. We, therefore, compared the full set of variants (113 SNPs) within the gene in a Portuguese dataset of 112 TB patients an...
Profiling of lung microbiota discloses differences in adenocarcinoma and squamo...
Gomes, Sílvia; Cavadas, Bruno; Ferreira, Joana Catarina; Marques, Patrícia Isabel; Monteiro, Catarina; Sucena, Maria; Sousa, CatarinaThe lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing...
NIS expression in thyroid tumors, relation with prognosis clinicopathological a...
Tavares, Catarina; Coelho, Maria João; Eloy, Catarina; Melo, Miguel; da Rocha, Adriana Gaspar; Pestana, Ana; Batista, Rui; Ferreira, Luciana BuenoThyroid cancer therapy is based on surgery followed by radioiodine treatment. The incorporation of radioiodine by cancer cells is mediated by sodium iodide symporter (NIS) (codified by the SLC5A5 gene), that is functional only when targeted to the cell membrane. We aimed to evaluate if NIS expression in thyroid primary tumors would be helpful in predicting tumor behavior, response to therapy and prognosis. NIS ...
CRABP1, C1QL1 and LCN2 are biomarkers of differentiated thyroid carcinoma, and ...
Celestino, Ricardo; Nome, Torfinn; Pestana, Ana; Hoff, Andreas M.; Gonçalves, A. Pedro; Pereira, Luísa; Cavadas, Bruno; Eloy, Catarina; Bjøro, TrineBackground: The prognostic variability of thyroid carcinomas has led to the search for accurate biomarkers at the molecular level. Follicular thyroid carcinoma (FTC) is a typical example of differentiated thyroid carcinomas (DTC) in which challenges are faced in the differential diagnosis. Methods: We used high-throughput paired-end RNA sequencing technology to study four cases of FTC with different degree of c...
Quantifying the legacy of the Chinese Neolithic on the maternal genetic heritag...
Brandão, Andreia; Eng, Ken Khong; Rito, Teresa S; Cavadas, Bruno; Bulbeck, David; Gandini, Francesca; Pala, Maria; Mormina, Maru; Hudson, BobThere has been a long-standing debate concerning the extent to which the spread of Neolithic ceramics and Malay-Polynesian languages in Island Southeast Asia (ISEA) were coupled to an agriculturally driven demic dispersal out of Taiwan 4000 years ago (4 ka). We previously addressed this question using founder analysis of mitochondrial DNA (mtDNA) control-region sequences to identify major lineage clusters most ...
Resolving the ancestry of Austronesian‑speaking populations
Soares, Pedro; Trejaut, Jean A.; Rito, Teresa S; Cavadas, Bruno; Hill, Catherine; Eng, Ken Khong; Mormina, Maru; Brandão, Andreia; Fraser, Ross M.There are two very different interpretations of the prehistory of Island Southeast Asia (ISEA), with genetic evidence invoked in support of both. The "out-of-Taiwan" model proposes a major Late Holocene expansion of Neolithic Austronesian speakers from Taiwan. An alternative, proposing that Late Glacial/postglacial sea-level rises triggered largely autochthonous dispersals, accounts for some otherwise enigmatic...
Fine time scaling of purifying selection on human nonsynonymous mtDNA mutations...
Cavadas, Bruno; Soares, Pedro; Camacho, Rui; Brandão, Andreia; Costa, Marta D; Fernandes, Verónica; Pereira, Joana B; Rito, Teresa S; Samuels, David CA high-resolution mtDNA phylogenetic tree allowed us to look backward in time to investigate purifying selection. Purifying selection was very strong in the last 2,500 years, continuously eliminating pathogenic mutations back until the end of the Younger Dryas (∼11,000 years ago), when a large population expansion likely relaxed selection pressure. This was preceded by a phase of stable selection until another ...