Encontrados 301 documentos, a visualizar página 1 de 31
Adaptation of key bacterial vaginosis-associated bacteria to a medium simulatin...
Sousa, Lúcia Filipa Guimarães Vieira; França, Ângela Maria Oliveira Sousa; Pinheiro, Vânia Oliveira; Muzny, Christina A.; Cerca, NunoTo study the influence of simulated in vivo conditions on the interactions of key BVAB, we analyzed the transcriptome of a triple-species biofilm composed of G. vaginalis, F. vaginae, and P. bivia, when biofilms were grown in a rich medium versus a medium mimicking genital tract secretions. Triple-species biofilms formed in mGTS were also analyzed by qPCR to determine each species percentage, revealing that the...
Key bacterial vaginosis-associated bacteria influence each others growth in bio...
Sousa, Lúcia Filipa Guimarães Vieira; Muzny, Christina A.; Cerca, NunoBacterial vaginosis (BV) is a very common gynaecologic condition affecting women of reproductive age worldwide. BV is characterized by a depletion of lactic acid-producing Lactobacillus species and an increase in strict and facultative anaerobic bacteria that develop a polymicrobial biofilm on the vaginal epithelium. Despite multiple decades over research, the etiology of this infection is still not clear. Howe...
Microbial interactions among Gardnerella, Prevotella and Fannyhessea prior to i...
Muzny, Christina A.; Elnaggar, Jacob H.; Sousa, Lúcia Filipa Guimarães Vieira; Lima, Ângela Martins; Aaron, Kristal J.; Eastlund, Isaac C.Introduction The aetiology of bacterial vaginosis (BV), a biofilm-associated vaginal infection, remains unknown. Epidemiologic data suggest that it is sexually transmitted. BV is characterised by loss of lactic acid-producing lactobacilli and an increase in facultative and strict anaerobic bacteria. Gardnerella spp are present in 95\%\textendash100\% of cases; Gardnerella vaginalis has been found to be more vir...
Sulfonyldibenzoate coordination polymers as bioactive dopants for polysaccharid...
Fernandes, Tiago A.; Macedo, Filipa; Cabral, Rafaela G.; Guiu, Telma; Franco, Chris H. J.; Jorge, Paula; Sousa, Ana Catarina; André, Vânia; Cerca, NunoThis work describes the preparation, characterization, and antimicrobial properties of bioactive silver(I) and copper(II) coordination polymers (bioCPs) and derived biopolymer materials. Two bioCPs, [Ag2(6-sdba)]n (1) and [Cu(4-sdba)H2O]n·1.5nH2O (2), were assembled from metal salt precursors and 4,4-sulfonyldibenzoic acid (H2sdba). Both compounds were used as dopants for preparing hybrid biopolymer films based...
Leveraging machine learning techniques to investigate the pathogenesis of incid...
Elnaggar, J.; Jacobs, C.; Ardizzone, C.; Aaron, K.; Eastlund, I.; Graves, K.; Luo, M.; Tamhane, A.; Long, D.; Laniewski, P.; Herbst-Kralovetz, M.Objective We investigated longitudinal changes in the vaginal microbiota prior to, during, and immediately following the onset of incident bacterial vaginosis (iBV) to investigate its pathogenesis. We used supervised machine learning and artificial neural networks (ANNs) to identify vaginal micro-organisms predictive of future iBV development. Study Design In this prospective cohort study, we performed 16S rRNA...
Deletion of codY impairs Staphylococcus epidermidis biofilm formation, generati...
Lopes, Nathalie; Pereira, Renato B; Correia, Alexandra; Vilanova, Manuel; Cerca, Nuno; França, Ângela Maria Oliveira SousaIntroduction. Staphylococcus epidermidis biofilms are one of the major causes of bloodstream infections related to the use of medical devices. The diagnosis of these infections is challenging, delaying their treatment and resulting in increased morbidity and mortality rates. As such, it is urgent to characterize the mechanisms employed by this bacterium to endure antibiotic treatments and the response of the ho...
Explorando os mistérios da vaginose bacteriana: como as espécies microbianas se...
Sousa, Lúcia Filipa Guimarães Vieira; França, Ângela Maria Oliveira Sousa; Muzny, Christina A.; Cerca, NunoA vaginose bacteriana (VB) é a infeção vaginal mais comum em mulheres em todo o mundo. A VB é caracterizada por um desequilíbrio na microbiota vaginal marcado por uma diminuição do número de espécies de Lactobacillus e um aumento do número de bactérias anaeróbias, que crescem num biofilme polimicrobiano. A formação de um biofilme polimicrobiano em casos de VB é uma das características mais associadas à VB, no e...
Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia strongly influ...
Sousa, Lúcia Filipa Guimarães Vieira; Novak, Juliano; França, Ângela Maria Oliveira Sousa; Muzny, Christina A.; Cerca, NunoBacterial vaginosis (BV), the most common vaginal infection worldwide, is characterized by the development of a polymicrobial biofilm on the vaginal epithelium. While Gardnerella spp. have been shown to have a prominent role in BV, little is known regarding how other species can influence BV development. Thus, we aimed to study the transcriptome of Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivi...
Optimized bacterial absolute quantification method by qPCR using an exogenous b...
Lameira, Inês; Pinto, Ana; Lima, Â.; Muzny, Christina A.; Cerca, NunoQuantitative Polymerase Chain Reaction (qPCR) is a widely used method in molecular biology to quantify target DNA sequences. Despite its accuracy, there are important experimental controls that should be considered to avoid biased results. One of them is gDNA loss during extraction, which is higher among samples with lower bacterial concentrations. Improvement in qPCR quantification procedures is mandatory to o...
Longitudinal analysis of select bacterial species composition among women with ...
George, S.; Dong, C.; Aaron, K.; Sherman, A.; Van Gerwen, O.; Tamhane, A.; Taylor, C.; Sousa, Lúcia Filipa Guimarães Vieira; Almeida, C.; Cerca, NunoObjective Key BV-associated bacteria that may be important in incident BV (iBV) pathogenesis are Gardnerella species and Prevotella bivia. We quantified the amount of these bacterial species in clue cell biofilms in longitudinal vaginal specimens from iBV cases and controls using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH). Study Design Women with optimal vaginal microbiota self-collected...