43 documents found, page 1 of 5
The effect of conditioned medium from human amniotic membrane-derived mesenchym...
Silva, C. S.; Casanova, Marta Alexandra Rodrigues; Ferreirinha, P.; da Silva Morais, A.; Osório, H.; Lima, A. C.; Fangueiro, J. F.; Cerqueira, M. T.Aims: The development and selection of T cells occur within the thymus. This organ involutes throughout life, compromising the generation of T cells and, consequently, the efficacy of the immune system. Mesenchymal stem cells (MSC) have beneficial effects on the immune system. Therefore, MSC have been applied in different pathologies related with thymic function. However, there is a lack of knowledge on the pot...
Skin in vitro models to study dermal white adipose tissue role in skin healing
Lago, M. E. L.; Cerqueira, M. T.; Pirraco, R. P.; Reis, R. L.; Marques, A. P.The perception of the role of adipose tissue has been changing over the years. For a long time, it was simply seen as an energy storage organ that confers mechanical protection and heat insulation. However, an increasing number of studies demonstrate the complex role of adipose tissue in the systemic regulation of processes such as immunity and inflammation. Therefore, adipose tissue is currently considered a m...
In vivo osteogenic differentiation of stem cells inside compartmentalized capsu...
Correia, C.; Santos, T. C.; Pirraco, R. P.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.Capsules coated with polyelectrolytes and co-encapsulating adipose stem (ASCs) and endothelial (ECs) cells with surface modified microparticles are developed. Microparticles and cells are freely dispersed in a liquified core, responsible to maximize the diffusion of essential molecules and allowing the geometrical freedom for the autonomous three-dimensional (3D) organization of cells. While the membrane wraps ...
Engineered hydrogel-based matrices for skin wound healing
Silva, L. P. da; Cerqueira, M. T.; Correlo, V. M.; Reis, R. L.; Marques, A. P.Hydrogels, due to their hygroscopic nature, have been widely used as wound dressings. Because hydrogels resemble native extracellular matrix (ECM), newer developments aim at recreating three-dimensional ECM-like microenvironments. In general, current hydrogels show limited molecular diffusion and cell binding sites. Spongy-like hydrogels, based on gellan gum polysaccharide, produced by a sequential advanced pro...
Stem cells in skin wound healing: are we there yet?
Cerqueira, M. T.; Pirraco, Rogério P.; Marques, A. P.Significance: Cutaneous wound healing is a serious problem worldwide that affects patients with various wound types, resulting from burns, traumatic injuries, and diabetes. Despite the wide range of clinically available skin substitutes and the different therapeutic alternatives, delayed healing and scarring are often observed. Recent Advances: Stem cells have arisen as powerful tools to improve skin wound heal...
Cell selective chitosan microparticles as injectable cell carriers for tissue r...
Custódio, Catarina A.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.The detection, isolation and sorting of cells holds an important role in cell therapy and regenerative medicine. Also, injectable systems have been explored for tissue regeneration in vivo, because it allows repairing complex shaped tissue defects through minimally invasive surgical procedures. Here we report the development of chitosan microparticles with a size of 115.8 m m able to capture and expand a speci ...
Stromal vascular fraction cell sheets angiogenic potential for tissue engineeri...
Costa, M.; Cerqueira, M. T.; Rodrigues, D. B.; Santos, T. C.; Marques, A. P.; Pirraco, Rogério P.; Reis, R. L.One of the biggest concerns in the Tissue Engineering field is the correct vascularization of engineered constructs. Strategies involving the use of endothelial cells are promising but adequate cell sourcing and neo-vessels stability are enduring challenges. In this work, we propose the hypoxic pre-conditioning of the stromal vascular fraction (SVF) of human adipose tissue to obtain highly angiogenic cell sheet...
Osteogenic differentiation of adipose stem cells by endothelial cells co-cultur...
Correia, Clara R.; Pirraco, Rogério P.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Mano, J. F.Inspired by the native co-existence of multiple cell types and from the concept of deconstructing the stem cell niche, we propose a co-encapsulation strategy within liquified capsules. The present team has already proven the application of liquified capsules as bioencapsulation systems1. Here, we intend to use the optimized system towards osteogenic differentiation. Capsules encapsulating adipose stem cells alo...
Skin-derived cell-sheets as powerful tools to engineer skin analogues
Cerqueira, M. T.; Pirraco, Rogério P.; Santos, T. C.; Reis, R. L.; Marques, A. P.Cell/cell-extracellular matrix (ECM) dynamic interactions appear to have a major role in regulating communication through soluble signaling, directing cell binding and activating substrates that participate in the highly organized wound healing process. Moreover, these interactions are also crucial for in vitro mimicking cutaneous physiology. Herein we explore cell sheet (CS) engineering to create cellular cons...
Cork extracts reduce UV-mediated DNA fragmentation and cell death
Araújo, A. R.; Pereira, D. M.; Aroso, Ivo Manuel Ascensão; Santos, T. C.; Batista, M. T.; Cerqueira, M. T.; Marques, A. P.; Reis, R. L.; Pires, R. A.UV radiation is known to induce the premature aging of human skin and to contribute to the occurrence of different skin cancers. High doses of UVA (able to penetrate through the epidermis into the dermis) and/or UVB radiation (only affecting the epidermis) leads to cellular oxidative damage compromising the recovery of the normal functions of the cells. This cellular damage is mainly driven by the generation of...