20 documents found, page 1 of 2
Potential of atlantic codfish (Gadus morhua) skin collagen for skincare biomate...
Rodrigues, Cristina V.; Sousa, Rita Alexandra Oliveira; Carvalho, Ana Cristina Pontes; Alves, Ana Luísa Silva; Marques, Ana Catarina FernandesCollagen is the major structural protein in extracellular matrix present in connective tissues, including skin, being considered a promising material for skin regeneration. Marine organisms have been attracting interest amongst the industry as an alternative collagen source. In the present work, Atlantic codfish skin collagen was analyzed, to evaluate its potential for skincare. The collagen was extracted from ...
Extracellular matrix in skin diseases: the road to new therapies
Malta, Mariana Jorge Oliveira Cardoso Denis; Cerqueira, Mariana Teixeira; Marques, A. P.Background: The extracellular matrix (ECM) is a vital structure with a dynamic and complex organization that plays an essential role in tissue homeostasis. In the skin, the ECM is arranged into two types of com- partments: interstitial dermal matrix and basement membrane (BM). All evidence in the literature sup- ports the notion that direct dysregulation of the composition, abundance or structure of one of thes...
Mechanomodulatory biomaterials prospects in scar prevention and treatment
Fernandes, Maria Goreti Antunes; da Silva, Lucília Pereira; Cerqueira, Mariana Teixeira; Ibanez, Rita; Murphy, Ciara M.; Reis, R. L.; OBrien, Fergal J.Scarring is a major clinical issue that affects a considerable number of patients. The associated problems go beyond the loss of skin functionality, as scars bring aesthetic, psychological, and social difficulties. Therefore, new strategies are required to improve the process of healing and minimize scar formation. Research has highlighted the important role of mechanical forces in the process of skin tissue re...
Rescuing key native traits in cultured dermal papilla cells for human hair rege...
Abreu, Carla M.; Cerqueira, Mariana Teixeira; Pirraco, Rogério P.; Gasperini, Luca; Reis, R. L.; Marques, A. P.Introduction: The dermal papilla (DP) represents the major regulatory entity within the hair follicle (HF), inducing hair formation and growth through reciprocal interactions with epithelial cells. However, human DP cells rapidly lose their hair inductive ability when cultured in an epithelium-deficient environment. Objectives: To determine if the conditioned medium collected from interfollicular keratinocytes ...
Interfollicular epidermal stem-like cells for the recreation of the hair follic...
Abreu, Carla M.; Pirraco, Rogério P.; Reis, R. L.; Cerqueira, Mariana Teixeira; Marques, A. P.Background Hair follicle (HF) development and growth are dependent on epithelial-mesenchymal interactions (EMIs). Dermal papilla (DP) cells are recognized as the key inductive mesenchymal player, but the ideal source of receptive keratinocytes for human HF regeneration is yet to be defined. We herein investigated whether human interfollicular epidermal keratinocytes with stem-like features (EpSlKCs), characteri...
Intracellular autofluorescence as a new biomarker for cancer stem cells in glio...
Castro, Joana Isabel Martins Cosme Vieira; Gonçalves, Celine Saraiva; Martins, Eduarda P.; Miranda-Lorenzo, Irene; Cerqueira, Mariana TeixeiraThe identification of cancer stem cells (CSCs), which are implicated in tumor initiation, progression, therapy resistance, and relapse, is of great biological and clinical relevance. In glioblastoma (GBM), this is still a challenge, as no single marker is able to universally identify populations of GBM cancer stem cells (GSCs). Indeed, there is still controversy on whether biomarker-expressing cells fulfill the...
Self-organized pigmented and vascularized full skin tissue model for melanoma r...
Soares, A. I.; Cerqueira, Mariana Teixeira; Pirraco, Rogério P.; Reis, R. L.; Marques, A. P.Being a multi-layered organ, the complexity of human skin is difficult to mimic in vitro. Despite the major advances in currently available 3D skin models, they remain very simplistic in relation to native tissue complexity. Moreover, the integration of melanoma cells into 3D skin models rendering representative tumor microenvironments is yet to be fully achieved. Cell sheet engineering has shown impressive res...
Extracellular matrix distinct signature among dystrophic epidermolysis bullosa ...
Malta, Mariana Jorge Oliveira Cardoso Denis; Osório, H.; Guttmann-Gruber, C.; Kocher, T.; Carvalho, A. F.; Cerqueira, Mariana Teixeira; Marques, A. P.Introduction & objectives: Mutations in the COL7A1 gene, which encodes collagen VII protein, the major component of the anchoring fibrils in the dermal-epidermal junction, cause all forms of dystrophic epidermolysis bullosa (DEB). Different clinical variants have been described with both dominant and recessive inheritance. However, information regarding the consequences of different COL7A1 mutations in the cell...
Mechanical Property of Hydrogels and the Presence of Adipose Stem Cells in Tumo...
Kundu, Banani; Bastos, A. R. F.; Brancato, V.; Cerqueira, Mariana Teixeira; Oliveira, Joaquim M.; Correlo, V. M.; Reis, R. L.; Kundu, Subhas COsteosarcoma is one of the most common metastatic bone cancers, which results in significant morbidity and mortality. Unfolding of effectual therapeutic strategies against osteosarcoma is impeded because of the absence of adequate animal models, which can truly recapitulate disease biology of humans. Tissue engineering provides an opportunity to develop physiologically relevant, reproducible, and tunable in vit...
Strategies for the hypothermic preservation of cell sheets of human adipose ste...
Freitas-Ribeiro, Sara; Carvalho, Andreia Filipa; Costa, Marina; Cerqueira, Mariana Teixeira; Marques, A. P.; Reis, R. L.; Pirraco, Rogério P.Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cel...