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Dynamic regulation of Grapevine’s microRNAs in response to Mycorrhizal Symbiosi...

Campos, Catarina; Coito, João Lucas; Cardoso, Hélia; Silva, Jorge Marques da; Pereira, Helena Sofia; Viegas, Wanda; Nogales, Amaia

MicroRNAs (miRNAs) are non-coding small RNAs that play crucial roles in plant development and stress responses and can regulate plant interactions with beneficial soil microorganisms such as arbuscular mycorrhizal fungi (AMF). To determine if root inoculation with distinct AMF species affected miRNA expression in grapevines subjected to high temperatures, RNA-seq was conducted in leaves of grapevines inoculated...


The effects of field inoculation of arbuscular mycorrhizal fungi through rye do...

Nogales, Amaia; Rottier, Emilien; Campos, Catarina; Victorino, Gonçalo; Costa, Joaquim Miguel; Coito, João Lucas; Pereira, H. Sofia; Viegas, Wanda

Grapevines are highly dependent on arbuscular mycorrhizal fungi (AMF) for normal growth and development. However, vineyard soils may have low AMF abundance and diversity due to conventional soil management practices that are detrimental for these fungi. In this context, the establishment of AMF-inoculated cover crops can be a highly convenient strategy to reestablish soil mycorrhizal potential, as it combines t...


The effects of field inoculation of arbuscular mycorrhizal fungi through rye do...

Nogales, Amaia; Rottier, Emilien; Campos, Catarina; Victorino, Gonçalo; Costa, Joaquim Miguel; Coito, João Lucas; Pereira, H. Sofia; Viegas, Wanda

Grapevines are highly dependent on arbuscular mycorrhizal fungi (AMF) for normal growth and development. However, vineyard soils may have low AMF abundance and diversity due to conventional soil management practices that are detrimental for these fungi. In this context, the establishment of AMF-inoculated cover crops can be a highly convenient strategy to reestablish soil mycorrhizal potential, as it combines t...


Deep analysis of wild Vitis flower transcriptome reveals unexplored genome regi...

Ramos, Miguel Jesus Nunes; Coito, João Lucas; Fino, Joana; Cunha, Jorge; Silva, Helena; Almeida, Patrícia Gomes; Costa, Maria Manuela Ribeiro

Key message RNA-seq of Vitis during early stages of bud development, in male, female and hermaphrodite flowers, identified new loci outside of annotated gene models, suggesting their involvement in sex establishment. Abstract The molecular mechanisms responsible for flower sex specification remain unclear for most plant species. In the case of V. vinifera ssp. vinifera, it is not fully understood what determine...


Flower development and sex specification in wild grapevine

Ramos, Miguel Jesus Nunes; Coito, João Lucas; Silva, Helena Gomes; Cunha, Jorge; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Background: Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed to have been first domesticated 10,000 years BC around the Caspian Sea. V. v. vinifera is hermaphrodite whereas V. v. sylvestris is a dioecious species. Male flowers show a reduced pistil without style or stigma and female flowers present reflexed stamens with infertile pollen. V. vinifera produce perfect ...


Flower development and sex specification in wild grapevine

Ramos, Miguel Jesus Nunes; Coito, João Lucas; Silva, Helena Gomes; Cunha, Jorge; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Background: Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed to have been first domesticated 10,000 years BC around the Caspian Sea. V. v. vinifera is hermaphrodite whereas V. v. sylvestris is a dioecious species. Male flowers show a reduced pistil without style or stigma and female flowers present reflexed stamens with infertile pollen. V. vinifera produce perfect ...


Flower development and sex specification in wild grapevine

Ramos, Miguel Jesus Nunes; Coito, João Lucas; Silva, Helena Gomes; Cunha, Jorge; Costa, Maria Manuela Ribeiro; Rocheta, Margarida

Background: Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed to have been first domesticated 10,000 years BC around the Caspian Sea. V. v. vinifera is hermaphrodite whereas V. v. sylvestris is a dioecious species. Male flowers show a reduced pistil without style or stigma and female flowers present reflexed stamens with infertile pollen. V. vinifera produce perfect ...


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