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First Insights on Resistance and Virulence Potential of Escherichia coli from C...

Magalhães, Rita; Abreu, Raquel; Pereira, Gonçalo; Cunha, Eva; Silva, Elisabete; Tavares, Luís; Chambel, Lélia; Oliveira, Manuela

Captive birds of prey are often used for pest control in urban areas, while also participating in falconry exhibitions. Traveling across the country, these birds may represent a public health concern as they can host pathogenic and zoonotic agents and share the same environment as humans and synanthropic species. In this work, Escherichia coli from the cloacal samples of 27 captive birds of prey were characteri...


The importance of “one health approach” to the AMR study and surveillance in An...

da Costa, Romay Coragem; Serrano, Isa; Chambel, Lélia; Oliveira, Manuela

The dissemination of multidrug-resistant (MDR) bacterial isolates in low- and middle-income countries, including several African countries, is a major concern. The poor sanitary conditions of rural and urban families observed in certain regions may favor the transmission of bacterial infections between animals and humans, including those promoted by strains resistant to practically all available antibiotics. In...


Biochar supplementation affects the microbiome of recycled manure solids for co...

Guerreiro, Joana F.; Pires, Ana J.; Nunes, Mónica; Esteves, Ana; Chambel, Lélia; Pascoal, Pedro; Pereira, Marcelo; Fangueiro, David; Tavares, Luís

The widespread use of Recycled Manure Solids (RMS) as cow bedding material is not without risks, since cattle manure may act as a vehicle for pathogenic and antimicrobial resistant bacteria dissemination. Thus, our aim was to evaluate RMS-supplemented with a pine biochar produced in Portugal as a new cow bedding material, since the use of biochar has been shown to have the potential to mitigate the impact of re...


In Vivo Effect of a Nisin–Biogel on the Antimicrobial and Virulence Signatures ...

Cunha, Eva; Ferreira, Ana Filipa; Valente, Sara; Matos, Alice; Carreira, L.Miguel; Videira, Marta; Chambel, Lélia; Tavares, Luis; Oliveira, Manuela

ABSTRACT - Periodontal disease is a relevant oral disease in dogs and nisin–biogel has been previously proposed to be used in its control. Enterococci, as inhabitants of the oral cavity with a high genetic versatility, are a reliable bacterial model for antimicrobial studies. Our goal was to evaluate the in vivo influence of the long-term dental application of the nisin–biogel on the virulence and antimicrobial...


Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflect...

Rosa, Ana Paula; Barão, Lúcia; Chambel, Lélia; Cruz, Cristina; Santana, Margarida

Drought is a worldwide problem, especially in arid and semi-arid regions. Detection of drought stress at the initial stages, before visible signs, to adequately manage irrigation and crop fertilization to avoid crop yield loss, is a desire of most farmers. Here, we evaluated the response of tomato plants to water scarcity, through changes in leaf reflectance due to modification in leaf pigments’ content, which ...


Early Identification of Plant Drought Stress Responses: Changes in Leaf Reflect...

Rosa, Ana Paula; Barão, Lúcia; Chambel, Lélia; Cruz, Cristina; Santana, Margarida

Drought is a worldwide problem, especially in arid and semi-arid regions. Detection of drought stress at the initial stages, before visible signs, to adequately manage irrigation and crop fertilization to avoid crop yield loss, is a desire of most farmers. Here, we evaluated the response of tomato plants to water scarcity, through changes in leaf reflectance due to modification in leaf pigments’ content, which ...


Aeromonas spp. Prevalence, virulence, and antimicrobial resistance in an ex sit...

Grilo, Miguel; Amaro, Guadalupe; Chambel, Lélia; Marques, Carolina S.; Marques, Tiago A.; Gil, Fátima; Sousa-Santos, Carla; Robalo, Joana

Ex situ breeding programs are important conservation tools for endangered freshwater fish. However, developing husbandry techniques that decrease the likelihood of disease, antimicrobial resistance, and virulence determinants acquisition during this process is challenging. In this pilot study, we conducted a captivity experiment with Portuguese nase (Iberochondrostoma lusitanicum), a critically endangered leuci...


Characterization of Escherichia coli isolates from water and food sold on the s...

Salamandane, Acácio; Alves, Suse; Chambel, Lélia; Malfeito-Ferreira, Manuel; Brito, Luísa

The aim of this study was to investigate the pathogenic potential and antibiotic resistance of 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) and drinking water (n = 28) sold in the city of Maputo, Mozambique. The isolates were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs were performed targeting five virulence genes (stx, lt, st, astA,...


Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas divers...

Grilo, Miguel; Chambel, Lélia; Marques, Tiago A.; Sousa-Santos, C.; Robalo, Joana; Oliveira, Manuela

Assessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity ...


Microbial Characterization of Yellow Curing Process of Codfish

Dias, Susana; Chambel, Lélia; Tenreiro, Rogério; Nunes, Leonor; Loureiro, Virgilio

Yellow cured codfish has a typical yellow colour, distinctive taste, and low salt content due to its special curing process of the raw salted codfish involving several soaks in water of the raw salted codfish, alternated with drying steps. The purpose of this study was to assess the main functional groups of bacteria involved in this process and relate them with physicochemical properties of the product. A tota...


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