15 documents found, page 1 of 2

Yeast as a tool to explore cathepsin D function

Cathepsin D has garnered increased attention in recent years, mainly since it has been associated with several human pathologies. In particular, cathepsin D is often overexpressed and hypersecreted in cancer cells, implying it may constitute a therapeutic target. However, cathepsin D can have both anti- and pro-survival functions depending on its proteolytic activity, cellular context and stress stimulus. There...

C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerev...

Specific ceramides are key regulators of cell fate, and extensive studies aimed to develop therapies based on ceramide-induced cell death. However, the mechanisms regulating ceramide cytotoxicity are not yet fully elucidated. Since ceramides also regulate growth and stress responses in yeast, we studied how different exogenous ceramides affect yeast cells. C2-phytoceramide, a soluble form of phytoceramides, the...

Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal memb...

Colorectal carcinoma (CRC) is one of the most common causes of cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from the intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant in CRC prevention and therapy. We previously reported that acetic acid-induced apoptosis in Saccharomyces cerevisiae cells involves partial vacuole permeabilization an...

Genome-wide identification of genes involved in the positive and negative regul...

Background: Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic acid triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies to modulate this process, to improve several biotechnological applications, but also for biomedical research. Indeed,...

What yeast can tell us about how cells commit suicide?

Multicellular organisms developed a complex system to balance cell proliferation and cell death in order to guarantee correct embryonic development and tissue homeostasis. Failure of cells to undergo programmed cell death (PCD) can potentially lead to severe diseases, including neural degeneration, autoimmunity and cancer. Identifying the molecules involved in PCD and understanding the regulation of the process...

Genome-wide identification of genes involved in the positive and negative regul...

Acetic acid is a weak organic acid that is a normal by-product of the alcoholic fermentation carried out by Saccharomyces cerevisiae. It is a common toxic agent present in different biotechnological processes, such as in wine or bioethanol production, and is frequently associated with impairment of fermentation 1,2. On the other hand, its toxic effects have prompted its use as a food preservative, but the resis...

The involvement of sphingolipids in apoptosis induced by acetic acid in yeast

Characterization of cisplatin-induced cell death in S. cerevisiae

MAPK signaling pathways mediate acetic acid-induced cell death in saccharomyces...

Yeast as a model system to study mechanisms regulating cisplatin sensitivity an...

Cisplatin is a highly effective chemotherapeutic drug used in the treatment of several tumors. It is a DNA-damaging agent that induces apoptosis of rapidly proliferating cells, an important factor underlying its therapeutic efficacy. Unfortunately, cellular resistance occurs often. A large fraction of tumor cells harbor mutations in p53, contributing to defects in apoptotic pathways and drug resistance. However...