8 documents found, page 1 of 1
Experimental models of scald burns. A scope review
Menegat,Taís Amadio; Oliveira,Andrea Fernandes de; Majewski,Michelle Gioia Coiado; Blanes,Leila; Juliano,Yara; Novo,Neil Ferreira; Ferreira,Lydia MasakoAbstract Purpose: To conduct a scope review of the experimental model described by Walker and Mason, by identifying and analyzing the details of the method. Methods: The authors searched Pubmed-Medline, Cochrane-Bireme and PEDro databases for articles published between January 2016 and December 2018, using the following search queries: burns, burn injuries, models animal, and animal experimentation. All article...
Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen sect...
Santos Filho,Ivan Dunshee de Abranches Oliveira; Enokihara,Milvia Maria Simões e Silva; Shie,Janaina Namba; Oliveira,Andrea Fernandes dePURPOSE: To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin. METHODS: Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were disse...
Development of experimental in vitro burn model
Fernandes,Ana Carolina Morais; França,Jerônimo Pereira de; Gaiba,Silvana; Aloise,Antonio Carlos; Oliveira,Andrea Fernandes dePURPOSE: To propose an experimental burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 wa...
Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman...
Oliveira,Andrea Fernandes de; Santos,Ivan Dunshee de Abranches Oliveira; Cartaxo,Sidney Bandeira; Bitar,Renata AndradePURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments ...
Perfusão isolada de membro com hipertermia na trataento das metástases em trâns...
Pasin,Victor Pavan; Oliveira,Andrea Fernandes de; Santos,Ivan Dunshee de Abranches Oliveira; Ferreira,Lydia MasakoOBJETIVO: Avaliar a casuística do Setor de Tumores Cutâneos da Disciplina de Cirurgia Plástica da Escola Paulista de Medicina/ Universidade Federal de São Paulo em relação à Perfusão Isolada de Membro (PIM) para o tratamento das metástases em trânsito do melanoma cutâneo, bem como os resultados conseqüentes, comparando-os com a literatura internacional. MÉTODO: De maio de 1993 a abril de 2007, 41 pacientes port...
Sentinel lymph node biopsy in cutaneous melanoma
Oliveira,Andrea Fernandes de; Santos,Ivan Dunshee de Abranches Oliveira; Tucunduva,Thaís Cardoso de Mello; Sanches,Luciana GarbeliniPURPOSE: To assess the importance of sentinel lymph node biopsy in patients with cutaneous melanoma. METHODS: Ninety consecutive non-randomized patients with stages I and II melanoma who underwent sentinel lymph node biopsy were followed up prospectively for six years. RESULTS: Patients were followed up for a mean period of 30 months. Their mean age was 53.3 years, ranging from 12 to 83 years. Thirty patients w...
Modelo experimental de cultura primária de melanoma metastático por punção aspi...
Oliveira,Andrea Fernandes de; Gragnani,Alfredo; Oliveira Filho,Renato Santos; Santos,Ivan Dunshee de Abranches Oliveira; França,Silvana Gaiba deOBJETIVO: Estabelecer um modelo experimental de cultura primária de melanoma metastático por punção aspirativa por agulha fina (PAAF). MÉTODOS: Duas culturas primárias de células de melanoma metastático através de PAAF foram desenvolvidas a partir de pacientes submetidos à excisão de lesões metastáticas e identificadas por imunohistoquímica utilizando os marcadores S-100 e MB-45, específicos para melanoma. RESU...
Perfuração espontânea de colédoco em adolescente
Pinto Júnior,Francisco Edilson Leite; Oliveira,Ariano José Freitas de; Oliveira,Andrea Fernandes deSpontaneous perforation of the common bile duct is rare. It happens predominantly in children and it is related to obstructive disease of the biliary tract. We present a case of an 18 year-old male patient, with ulcerative rectocolitis associated with malignant tumor of the head of pancreas. The patient developed an acute abdomen syndrome and laparotomy, a spontaneous perforation of common bile duct was evidenc...