15 documents found, page 1 of 2
Unveiling the effect of three- dimensional bioactive fibre mesh scaffolds funct...
Rodrigues, A. I.; Franco, A. R.; Rodrigues, F.; Leonor, I. B.; Reis, R. L.There is an increasing clinical need to design novel biomaterials that combine bone regenerative potential with antibacterial properties. Silicon-based biomaterials, widely studied for application in bone regeneration, have demonstrated antibacterial properties. Herein, the aim of this work was to investigate the potential of the functionalization of biomaterials surfaces with silanol groups to prevent the bact...
Designing biomaterials based on biomineralization for bone repair and regeneration
Leonor, I. B.; Rodrigues, A. I.; Reis, R. L.The research work presented in this chapter highlights the importance of under- standing the surface chemistry of biomaterials, as well as the main mechanism for apatite formation, which provides the fundamental principles and tools for designing new bioactive materials. These fundamental mechanisms of bioactive ceramics are believed to open a new avenue for future breakthroughs for clinical biointeractive mate...
New microbial surface-active compounds: the ultimate alternative to chemical su...
Gudiña, Eduardo J.; Rodrigues, A. I.; Teixeira, J. A.; Rodrigues, L. R.Surface active compounds (SACs) produced by microorganisms are attracting a pronounced interest due to their potential advantages over synthetic counterparts, and to the fact that they could replace some of the synthetics in many environmental and industrial applications. Bioemulsifier production by a Paenibacillus strain isolated from crude oil was studied. The bioemulsifier was produced using sucrose with and...
Biosurfactant production by Bacillus subtilis using corn steep liquor as cultur...
Gudiña, Eduardo J.; Fernandes, Elisabete Clara Resende; Rodrigues, A. I.; Teixeira, J. A.; Rodrigues, L. R.In this work, biosurfactant production by Bacillus subtilis #573 was evaluated using corn steep liquor (CSL) as culture medium. The best results were obtained in a culture medium consisting of 10% (v/v) of CSL, with a biosurfactant production of about 1.3 g/l. To the best of our knowledge, this is the first report describing biosurfactant production by B. subtilis using CSL as culture medium. Subsequently, the ...
Development of low-cost culture media for effective biosurfactant production
Rodrigues, A. I.; Gudiña, Eduardo J.; Teixeira, J. A.; Rodrigues, L. R.In this work, biosurfactant production by Pseudomonas aeruginosa and Bacillus subtillis strains was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/L) by the P. aeruginosa strain was obtained using a culture medium containing corn steep liquor (CSL) (10% (v/v)) and molasses (10% (w/v)), whereas the best biosurfactant production by the B. subtillis isolate (1.3 g/L) was obtained ...
Elucidating the individual effects of calcium and phosphate ions on hMSCs by us...
Danoux, C. B.; Bassett, David C.; Othman, Ziryan; Rodrigues, A. I.; Reis, R. L.; Barralet, Jake E.; Blitterswijk, C. A. van; Habibovic, PamelaProva tipográfica; The biological performance of bone graft substitutes based on calcium phosphate bioceramics is dependent on a number of properties including chemical composition, porosity and surface micro- and nanoscale structure. However, in contemporary bioceramics these properties are interlinked, therefore making it difficult to investigate the individual effects of each property on cell behavior. In th...
Bioconversion of agro-industrial by-products in rhamnolipids toward application...
Gudiña, Eduardo J.; Rodrigues, A. I.; Alves, Eliana; Domingues, M. R. M.; Teixeira, J. A.; Rodrigues, L. R.In this work, biosurfactant production by a Pseudomonas aeruginosa strain was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/l) was obtained using a culture medium containing corn step liquor (10% (v/v)) and molasses (10% (w/v)). The biosurfactant reduced the surface tension of water up to 30 mN/m, and exhibited a high emulsifying activity (E24= 60%), with a critical micelle co...
Stimulatory effects of inorganic ions on osteogenesis in vitro
Rodrigues, A. I.; Leonor, I. B.; Reis, R. L.; van Blitterswijk, C.; Habibovic, P.Introduction: Several studies demonstrated the effect of silicate ions (Si) on differentiation of bone precursor cells1,2, although its exact role in processes related to bone formation and remodeling is still incompletely understood. The focus of this work is to explore the effect of calcium and silicate ions on growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs). This strategy may re...
Biodegradation of ochratoxin A by Pediococcus parvulus isolated from Douro wines
Abrunhosa, Luís; Inês, António; Rodrigues, A. I.; Guimarães, Ana Cristina Silva Esperança; Pereira, Vânia L.; Parpot, Pier; Mendes-Faia, ArleteLactic acid bacteria (LAB) are a promising solution to reduce exposure to dietary mycotoxins because of the unique mycotoxin decontaminating characteristic of some LAB. Ochratoxin A (OTA) is one of the most prominent mycotoxins found in agricultural commodities. The present work reports on the ability of Pediococcus parvulus strains that were isolated from Douro wines that spontaneously underwent malolactic fer...
Improved biosurfactant production by a Pseudomonas aeruginosa strain using agro...
Gudiña, Eduardo J.; Rodrigues, A. I.; Teixeira, J. A.; Rodrigues, L. R.Microbial surfactants are amphipathic molecules produced by a variety of microorganisms that exhibit pronounced surface and emulsifying activities. Biosurfactants can replace synthetic surfactants in environmental and industrial applications, such as bioremediation and microbial enhanced oil recovery. Furthermore, some biosurfactants have been reported as suitable alternatives to synthetic medicines and antimic...