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Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
Chiari,Egler; Dias,João Carlos Pinto; Lana,Marta; Chiari,Clea AndradeWith the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS whic...
Date: 1989
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Origin: Oasisbr