19 documents found, page 1 of 2
Comparison of phenol-chloroform and a commercial deoxyribonucleic acid extracti...
Silva,Andressa Noronha Barbosa da; Souza,Rita de Cássia Moreira de; Honorato,Nathan Ravi Medeiros; Martins,Rand RandallAbstract INTRODUCTION: Knowledge of triatomine bloodmeal sources is essential for understanding vector-host interactions in Trypanosoma cruzi transmission cycles. Expensive commercial deoxyribonucleic acid (DNA) extraction kits are widely used for bloodmeal identification. This study assessed the performance of an inexpensive phenol-chloroform DNA extraction protocol for identification of triatomine bloodmeal s...
Synanthropic triatomines (Hemiptera: Reduviidae): infestation, colonization, an...
Barbosa-Silva,Andressa Noronha; Souza,Rita de Cássia Moreira de; Diotaiuti,Liléia; Aguiar,Lúcia Maria Abrantes; Câmara,Antonia Cláudia Jácome daAbstract INTRODUCTION The ecoepidemiological situation in the State of Rio Grande do Norte, Brazil is characterized by frequent invasion and colonization of domiciliary units (DUs) by several triatomine species, with high rates of natural infection by Trypanosoma cruzi. METHODS: We evaluated the possibility of vector transmission of T. cruzi based on records of the occurrence of domiciled triatomines collected ...
Combined parasitological and molecular-based diagnostic tools improve the detec...
Volpato,Fabiana Caroline Zempulski; Sousa,Giovane Rodrigo; D’Ávila,Daniella Alchaar; Galvão,Lúcia Maria da Cunha; Chiari,EglerAbstract INTRODUCTION In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. METHODS Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, foll...
Characteristics of Triatomine infestation and natural Trypanosoma cruzi infecti...
Barbosa-Silva,Andressa Noronha; Câmara,Antonia Cláudia Jácome da; Martins,Kiev; Nunes,Daniela Ferreira; Oliveira,Pedro Igor Câmara deAbstract: INTRODUCTION Natural and artificial ecotope infestation by the kissing bug triatomines and their colonization and infection by Trypanosoma cruzi , the Chagas disease agent, were evaluated in nine municipalities of the State of Rio Grande do Norte, Brazil. METHODS Following identification, triatomine intestinal contents were analyzed by direct microscopic examination, xenoculture, and polymerase chain ...
Chagas disease: morbidity profile in an endemic area of Northeastern Brazil
Andrade,Cléber de Mesquita; Câmara,Antônia Cláudia Jácome da; Nunes,Daniela Ferreira; Guedes,Paulo Marcos da Matta; Pereira,Wogelsanger OliveiraAbstract: INTRODUCTION : This study evaluated the clinical forms and manifestation severities of Chagas disease among serologically reactive individuals from Western Rio Grande do Norte (Northeastern Brazil). METHODS : This cross-sectional study included 186 adults who were evaluated using electrocardiography, echocardiography, chest radiography, and contrast radiography of the esophagus and colon. A clinical-e...
Seroepidemiology of Trypanosoma cruzi infection in the semiarid rural zone of t...
Brito,Carlos Ramon do Nascimento; Sampaio,George Harisson Felinto; Câmara,Antonia Cláudia Jácome da; Nunes,Daniela FerreiraINTRODUCTION: A seroepidemiological survey was carried out to evaluate Trypanosoma cruzi infection in an endemic area of the State of Rio Grande do Norte, Brazil, involving rural residents. METHODS: Sixteen municipalities were randomly selected, 15 from the west mesoregion and one from the central, with an estimated population of 83,852 individuals. A total of 1,950 blood samples were collected in the west meso...
Hematological alterations during experimental canine infection by Trypanosoma c...
Guedes,Paulo Marcos Matta; Veloso,Vanja Maria; Mineo,Tiago Wilson Patriarca; Santiago-Silva,Juliana; Crepalde,Geovan; Caldas,Ivo SantanaTo confirm that Beagle dogs are a good experimental model for Chagas disease, we evaluated hematological alterations during the acute and chronic phases in Beagle dogs infected with the Y, Berenice-78 (Be-78) and ABC strains of Trypanosomacruzi, correlating clinical signs with the parasitemia curve. We demonstrate that the acute phase of infection was marked by lethargy and loss of appetite. Simultaneously, we ...
Synthesis and trypanocidal activity of 7, 2'-dioxygenated isoflavones and oxypr...
Faria,Terezinha de J.; Silva,Luiz G. Fonseca e; Souza Filho,José D. de; Chiari,Egler; Oliveira,Alaíde B. de7, 2'-Dihydroxyisoflavone was shown to be active against the bloodstream trypomastigote form of Trypanosoma cruzi, the etiologic agent of Chagas' disease. Amphiphilic derivatives of this isoflavone were synthezised aiming to obtain hydrosoluble compounds of potential use as prophylactic agents to be added to blood for transfusion. This isoflavone was obtained by demethylation of 7-hydroxy-2'-methoxyisoflavone t...
Preparation and activity of diterpenoids against trypomastigotes of Trypanosoma...
Takahashi,Jacqueline A.; Vieira,Henriete S.; Silva,Eliane A.; Boaventura,Maria A. D.; Oliveira,Alaíde B. de; Chiari,EglerA systematic investigation on the trypanocidal effect of several natural products isolated from Brazilian plant species has been carried out. In this paper we report on the results obtained from the screening of 26 diterpenes from natural sources or of synthetic/microbial transformations origin (mainly derivatives of kaurenoic acid) against trypomastigote forms of Trypanosoma cruzi, the causative agent of Chaga...
Aminoquinolone WR6026 as a feasible substitute for gentian violet in Chagas' di...
Moraes-Souza,Helio; Pianetti,Gisele Marilia; Barretto,Orlando César de Oliveira Pereira; Nonoyama,Kimyo; Grolg,M.; Chiari,EglerThe search for a colorless, nontoxic and efficient drug to prevent transfusion-associated Chagas' disease (TACD) has been underway unsuccessfully since 1953 when gentian violet was preconized and to date is still being used as the only in vitro trypanocidal agent. The recent findings of aminoquinolone "WR6026" as a trypanocidal agent, led the authors to study the metabolism of red cells stored with this compoun...