4 documents found, page 1 of 1
Bioinstructive Layer-by-Layer-Coated Customizable 3D Printed Perfusable Microch...
Sousa, Cristiana F. V.; Saraiva, Catarina A.; Correia, Tiago R.; Pesqueira, Tamagno; Patrício, Sónia G.; Rial-Hermida, Maria Isabel; Borges, JoãoThe development of complex and large 3D vascularized tissue constructs remains the major goal of tissue engineering and regenerative medicine (TERM). To date, several strategies have been proposed to build functional and perfusable vascular networks in 3D tissue-engineered constructs to ensure the long-term cell survival and the functionality of the assembled tissues after implantation. However, none of them ha...
Superhydrophobic chips for cell spheroids high-throughput generation and drug s...
Oliveira, Mariana B.; Neto, Ana I.; Correia, Clara R.; Rial-Hermida, Maria Isabel; Álvarez-Lorenzo, Carmen; Mano, J. F.We suggest the use of biomimetic superhydrophobic patterned chips produced by a bench-top methodology as low-cost and waste-free platforms for the production of arrays of cell spheroids/microtissues by the hanging drop methodology. Cell spheroids have a wide range of applications in biotechnology fields. For drug screening, they allow studying 3D models in structures resembling real living tissues/tumors. In ti...
Bioinspired superamphiphobic surfaces as a tool for polymer-and solvent-indepen...
Rial-Hermida, Maria Isabel; Oliveira, Nuno M.; Concheiro, Angel; Alvarez-Lorenzo, Carmen; Mano, J. F.Superamphiphobic surfaces were evaluated as a tool to prepare spherical particles from polymers and solvents of very diverse nature, under mild conditions and with 100% drug encapsulation yield. Different from bioinspired superhydrophobic surfaces suitable only for aqueous dispersions, the superamphiphobic platforms allowed the formation of spherical droplets when solvents of any polarity were deposited onto th...
High-throughput drug screening using cell spheroids in superhydrophobic pattern...
Oliveira, Mariana B.; Neto, Ana I.; Rial-Hermida, Maria Isabel; Alvarez-Lorenzo, Carmen; Mano, J. F.High-throughput studies in biotechnology areas such as drug screening and tissue engineering have been carried out mainly in 2D environment. However, the low clinical and biological relevance of 2D models is well known. Both in tissues and tumor masses, cells lie in a 3D configuration organized in the self-secreted microenvironment: the extracellular matrix (ECM). In this milieu, cells interact in a totally nat...