Worldwide sustainable development is threatened by current agricultural land change trends, particularly by the increasing rural farmland abandonment and agricultural intensification phenomena. In Mediterranean countries, these processes are a ecting especially traditional olive groves with enormous socio-economic costs to rural areas, endangering environmental sustainability and biodiversity. Traditional olive...
The current symbiotic view of the organisms also calls for new approaches in the way we perceive and manage our pest species. The olive fruit fly, the most important olive tree pest, is dependent on an obligate bacterial symbiont to its larvae development in the immature fruit. This symbiont, Candidatus (Ca.) Erwinia dacicola, is prevalent throughout the host life stages, and we have shown significant changes i...
Prays oleae is the second most important pest in Mediterranean olive groves, causing substantial damage on olive production. We used mitochondrial [cytochrome c oxidase subunit I (COI), and NADH dehydrogenase subunit 5 (nad5)] and nuclear [ribosomal protein S5 (RpS5)] amplicons to assess the population variability in five main olive producing regions from Tunisia, to support or dismiss the existence of two non-...
The European grapevine moth, Lobesia botrana, has high economic impact on grapevines being one of the most harmful pests of vineyards worldwide. Wolbachia infection has not yet been reported for this moth. We systematically look for Wolbachia presence in L. botrana from three areas within the viticultural region of Alentejo (Portugal), via wsp gene specific PCR, and consistently found its presence. The interact...
Over the past few decades, species distribution modelling has been increasingly used to monitor invasive species. Studies herein propose to use Cellular Automata (CA), not only to model the distribution of a potentially invasive species but also to infer the potential of the method in risk prediction of Reticulitermes grassei infestation. The test area was mainland Portugal, for which an available presence-only...
A new Terfezia species-Terfezia solaris-libera sp. nov., associated with Tuberaria guttata (Cistaceae) is described from Alentejo, Portugal. T. solaris-libera sp. nov. distinct morphology has been corroborated by its unique ITS-rDNA sequence. Macro and micro morphologic descriptions and phylogenetic analyses of ITS data for this species are provided and discussed in relation to similar spiny-spored species in t...
Desert truffles mycorrhizal hypogeous Ascomycota are found in arid and semi arid areas of the globe and have great ecological and economic importance Terfezia is undoubtedly the most diversified of all desert truffle genera, but its taxonomy is far from resolved Specifically,
Desert truffles are of considerable interest for ecological, agroforestry and commercial purposes They represent a key component of the mycobiota in arid and semi arid regions, due to their important role as symbiotic partners of diverse host plants, most often members of the Cistaceae Plus,their fruitbodies constitutes a potentially important food source for animals and humans, rich in proteins and poor in car...
Developing below the soil surface desert, truffles are hard to find. Within Terfezia genus, at least 18 species are described and many are endemic to the Mediterranean basin. Ecological and geographic information are key factors for species diagnosis, and so far Terfezia species are believed to be linked to either acidic or basic soils or to specific plant hosts. Thus, we have looked at Terfezia diversity withi...
Symbiotic-based pest management strategies are promising but require previous knowledge on the microbial community structure and on key microorganisms. Highly specialised pests, as the monophagous olive moth, are likely to have co-evolved microbiota that aid in overcoming specific plant defences that have emerged from the tight insect-plant interaction. Here we report a first attempt to identify olive moth bact...