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Establishment of a Protocol for the Characterization of Secreted Biomolecules i...

Pires, Rita; Rodrigues, Lénia; Santos, Fátima; Duarte, Iola; Ciordia, Sérgio; Peixe, Augusto; Cardoso, Hélia

Somatic embryogenesis (SE) involves the formation of embryo-like structures from somatic cells without fertilization and is widely used for clonal propagation and genetic transformation. However, in olive (Olea europaea sp. europaea), SE remains challenging due to the recalcitrant behavior of adult tissues when used as initial explants. Bioactive molecules released into the culture medium (conditioned medium, C...


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