5 documents found, page 1 of 1
Extracellular vesicles from pancreatic cancer stem cells lead an intratumor com...
Ruivo, Carolina F.; Bastos, Nuno; Adem, Barbara; Batista, Ines; Duraes, Cecilia; Melo, Carlos A.; Castaldo, Stephanie A.; Campos‐Laborie, FranciscoObjective: Intratumor heterogeneity drives cancer progression and therapy resistance. However, it has yet to be determined whether and how subpopulations of cancer cells interact and how this interaction affects the tumour. Design: We have studied the spontaneous flow of extracellular vesicles (EVs) between subpopulations of cancer cells: cancer stem cells (CSC) and non-stem cancer cells (NSCC). To determine th...
iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal De...
Santos, F.M.; Gaspar, Leonor Isabel Mesquita; Ciordia, Sergio; Rocha, Ana; Sousa, João Paulo Castro De; Paradela, Alberto; Passarinha, LA; Tomaz, C. T.Rhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was c...
Protein markers of Bursaphelenchus xylophilus Steiner & Buhrer, 1934 (Nickle, 1...
Ciordia, Sergio; Robertson, Lee; Arcos, Susana; Gonzales, Maria Rosa; Mena, Maria del Carmen; Zamora, Paula; Vieira, Paulo; Abrantes, IsabelThe Pine Wood Nematode (PWN) Bursaphelenchus xylophilus is a severe forest pathogen in countries where it has been introduced and is considered a worldwide quarantine organism. In this study, protein markers for differentiating populations of this nematode were identified by studying differences among four selected Iberian and one American population. These populations were compared by quantitative proteomics (...
Halorhabdus tiamatea : proteogenomics and glycosidase activity measurements ide...
Werner, Johannes; Ferrer, Manuel; Michel, Gurvan; Mann, Alexander J.; Sixing Huang; Juarez, Silvia; Ciordia, Sergio; Albar, Juan P.; Alcaide, MaríaEuryarchaea from the genus Halorhabdus have been found in hypersaline habitats worldwide, yet are represented by only two isolates: Halorhabdus utahensis AX-2T from the shallow Great Salt Lake of Utah, and Halorhabdus tiamatea SARL4BT from the Shaban deep-sea hypersaline anoxic lake (DHAL) in the Red Sea. We sequenced the H. tiamatea genome to elucidate its niche adaptations. Among sequenced archaea, H. tiamate...
SELDI-TOF biomarker signatures for cystic fibrosis, asthma and chronic obstruct...
Gomes-Alves, Patrícia; Imrie, Margaret; Gray, Robert D.; Nogueira, Paulo; Ciordia, Sergio; Pacheco, Paula; Azevedo, Pilar; Lopes, CarlosOBJECTIVES: The aim of this work was to establish protein profiles in serum and nasal epithelial cells of cystic fibrosis individuals in comparison with controls, asthma and chronic obstructive pulmonary disease patients for specific biomarker signatures identification. DESIGN AND METHODS: Protein extracts were analyzed by Surface Enhanced Laser Desorption/Ionization Time-Of-Flight Mass-Spectrometry (SELDI-TOF-...