26 documents found, page 1 of 3
Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisi...
Sattler, Mariana Cansian; Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo; Sanglard, Natália Arruda; Amaral-Silva, Paulo MarcosIndirect somatic embryogenesis (ISE) is required for plant propagation and a prerequisite for applications that may provide new germplasms. Genetic, epigenetic and physiological features of the explant donor are barriers for ISE establishment, hindering its wide use. Despite the identification and/or expression analysis of genes during ISE, no approach to establish the karyotype aspects has been performed so fa...
Diversidade genética estimada com marcadores entre sequências simples repetidas...
Oliveira, Luiz Orlando de; Silva, Bruna Mezzalira da; Rossi, Ana Aparecida Bandini; Dardengo, Juliana de Freitas EncinasQuinze primers ISSR (entre sequências simples repetidas) foram utilizados para avaliar a diversidade genética entre e dentro de pomares comerciais de Theobroma grandiflorum (Willd. ex Spreng.) K. Schum. Para isso, foram analisados sessenta indivíduos, distribuídos nos três cultivos. Um total de 102 bandas foi amplificado, com uma porcentagem de 52,0% de polimorfismo em nível de espécie e média de 6,8 alelos por...
The first karyogram of a Bromeliaceae species: an allopolyploid genome
Carvalho, Carlos Roberto; Nunes, Andrei Caíque Pires; Nogueira, Ester Ujiie; Gontijo, Andreia Barcelos Passos Lima; Clarindo, Wellington RonildoThe Bromeliaceae family has been traditionally distributed in the subfamilies Bromelioideae, Tillandsioideae and Pitcairnioideae. However, phylogenetic studies have provided other classifications, highlighting the need for analyses in order to characterize the genome of different species from this family. In this sense, the present work aimed to determine nuclear 2C-value and base composition, characterize the ...
Embryogenic potential of immature zygotic embryos of Passiflora: a new advance ...
Carvalho, Carlos Roberto; Ferreira, Darley Aparecido Tavares; Sattler, Mariana Cansian; Clarindo, Wellington RonildoIn vitro strategies for Passiflora have been developed owing to its economic and ecological importance. However, plantlet regeneration through somatic embryogenesis has presented some problems, such as the reproducibility of the protocol and formation of abnormal embryos and plantlets. Thus, this study aimed to establish a protocol exploring the embryogenic potential of immature zygotic embryos (IZE) of the wil...
Karyotype revised of Pisum sativum using chromosomal DNA amount
Carvalho, Carlos Roberto; Fontes, Milene Miranda Praça; Clarindo, Wellington RonildoPisum sativum was one of the first plants for which the mitotic karyotype was recognized and the karyogram assembled. These achievements were required owing to the physical mapping of P. sativum, providing data for evolutionary approaches and breeding programs. In spite of significant advances, precise morphometric characterization of chromosomes and karyogram assembly of P. sativum have become a topical proble...
Ploidy instability in long-term in vitro cultures of Coffea arabica L. monitore...
Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo; Mendonça, Maria Andréia CorrêaSomatic embryogenesis of Coffea arabica L. has been mainly carried out in liquid medium for clonal and mass propagation of elite lines. This in vitro system involves suspension cultures of embryogenic aggregates, with high multiplication rate and unorganized growth. These characteristics are linked to the occurrence of somaclonal variation (SV), especially considering that cell aggregates are usually maintained...
In vitro responses in Passiflora species with different chromosome numbers, plo...
Carvalho, Carlos Roberto; Clarindo, Wellington Ronildo; Ferreira, Adésio; Praça-Fontes, Milene Miranda; Vieira, Ariane TonettoTissue culture in Passiflora has emerged as a strategy to propagate species with agronomic relevance, which is the main focus of most in vitro studies. Different morphogenic responses have been obtained under the same environmental in vitro conditions, mainly for species of the subgenus Passiflora with distinct 2n chromosome numbers. The aims of this study were to verify and compare the in vitro responses in Pa...
From chromosome doubling to DNA sequence changes: outcomes of an improved in vi...
Sattler, Mariana Cansian; Carvalho, Carlos Roberto; Nunes, Andrei Caíque Pires; Sanglard, Natália Arruda; Silva, Paulo Marcos AmaralSince 1966, chromosome doubling has been performed mainly in vitro, associating anti-tubulin treatment and different plant tissues showing proliferative cells. Despite the achieved improvements, some bottlenecks have been pointed out, such as the low rate of polyploids and high rate of mixoploid plantlets. To overcome these hurdles, some approaches have indicated that indirect somatic embryogenesis (ISE) consti...
Flow cytometric analysis using SYBR Green I for genome size estimation in coffee
Clarindo, Wellington Ronildo; Carvalho, Carlos RobertoPlant genome size has been measured by flow cytometry using propidium iodide as a dye for nuclear DNA staining. However, some authors have reported the occurrence of genome size estimation errors, especially in plants rich in secondary metabolites, such as the coffee tree. In this context, we tested an alternative cytometric protocol using the SYBR Green I as a fluorochrome for stoichiometrically staining nucle...
The polyploidy and its key role in plant breeding
Sattler, Mariana Cansian; Carvalho, Carlos Roberto; Clarindo, Wellington RonildoPolyploidy is a major force in the evolution of both wild and cultivated plants. Polyploid organisms often exhibit increased vigor and, in some cases, outperform their diploid relatives in several aspects. This remarkable superiority of polyploids has been the target of many plant breeders in the last century, who have induced polyploidy and/or used natural polyploids in many ways to obtain increasingly improve...