30 documents found, page 1 of 3
Source attribution of human infection: a multi-country model in the European Union
Thystrup, Cecilie; Brinch, Maja Lykke; Henri, Clementine; Mughini-Gras, Lapo; Franz, Eelco; Wieczorek, Kinga; Gutierrez, MontserratIntroduction: Infections caused by spp. represent a severe threat to public health worldwide. National action plans have included source attribution studies as a way to quantify the contribution of specific sources and understand the dynamic of transmission of foodborne pathogens like and . Such information is crucial for implementing targeted intervention. The aim of this study was to predict the sources of hu...
Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis fr...
Leão, Célia; Silveira, Leonor; Usié, Ana; Gião, Joana; Clemente, Lurdes; Themudo, Patricia; Amaro, Ana; Pista, AngelaSalmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the leading causes of foodborne infections associated with broilers and laying hens. Portugal has had the lowest notification rates of salmonellosis in recent years, due to the vaccinations of layer and breeder flocks and strict compliance with biosecurity measures. However, data about the genetic diversity of S. Enteritidis in P...
Building an International One Health Strain Level Database to Characterise the ...
Perestrelo, Sara; Amaro, Ana; Brouwer, Michael S.M.; Clemente, Lurdes; Ribeiro Duarte, Ana Sofia; Kaesbohrer, Annemarie; Karpíšková, RenataAntimicrobial resistance (AMR) is one of the top public health threats nowadays. Among the most important AMR pathogens, Escherichia coli resistant to extended spectrum cephalosporins (ESC-EC) is a perfect example of the One Health problem due to its global distribution in animal, human, and environmental sources and its resistant phenotype, derived from the carriage of plasmid-borne extended-spectrum and AmpC ...
First comparative genomic characterization of the MSSA ST398 lineage detected i...
Salgueiro, Vanessa; Manageiro, Vera; Bandarra, Narcisa M.; Ferreira, Eugénia; Clemente, Lurdes; Caniça, ManuelaStaphylococcus aureus ST398 can cause diseases in several different animals. In this study we analyzed ten S. aureus ST398 previously collected in three different reservoirs in Portugal (humans, gilthead seabream from aquaculture and dolphin from a zoo). Strains tested against sixteen antibiotics, by disk diffusion or minimum inhibitory concentration, showed decreased susceptibility to benzylpenicillin (all str...
A deixis como ferramenta de análise do texto literário
Jorge, Noémia de Oliveira; Viegas, Filomena; Clemente, Lurdes; Castelão, Maria Manuela; Almeida, Paulo Duarte deApresentamos, neste texto, uma breve reflexão sobre a forma como a deixis pode ser encarada não (apenas) como mero conteúdo gramatical a abordar explicitamente nas aulas de Português do 11.º ano de escolaridade, mas também como “ferramenta” a ser colocada ao serviço da análise de textos literários contribuindo para o desenvolvimento da competência de leitura literária1. Assim, num primeiro momento faremos um br...
Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reser...
Salgueiro, Vanessa; Manageiro, Vera; Bandarra, Narcisa M.; Ferreira, Eugénia; Clemente, Lurdes; Caniça, ManuelaThe main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and...
Revealing mcr-1-positive ESBL-producing Escherichia coli strains among Enteroba...
Clemente, Lurdes; Manageiro, Vera; Correia, Ivone; Amaro, Ana; Albuquerque, Teresa; Themudo, Patrícia; Ferreira, Eugénia; Caniça, ManuelaWe screened 1840 Enterobacteriaceae isolates from food-producing animals, meat, meat products and animal feed, for the detection of plasmid-mediated colistin resistance, during 2010-2015. The mcr-1 gene was detected in 8.0% (97/1206) Escherichia coli and in 0.47% (3/634) Salmonella enterica isolates, with a high number of mcr-1 positive E. coli isolates (45.7%) being extended-spectrum β-lactamase or plasmid-med...
IncX4 plasmid carrying the new mcr-1.9 gene variant in a CTX-M-8-producing Esch...
Manageiro, Vera; Clemente, Lurdes; Romão, Raquel; Silva, Catarina; Vieira, Luís; Ferreira, Eugénia; Caniça, ManuelaWe studied a commensal colistin-resistant Escherichia coli isolated from a swine cecum sample collected at a slaughter, in Portugal. Antimicrobial susceptibility phenotype of E. coli LV23529 showed resistance to colistin at a minimum inhibitory concentration of 4 mg/L. Whole genome of E. coli LV23529 was sequenced using a MiSeq system and the assembled contigs were analyzed for the presence of antibiotic resist...
Biochemical characterization of CTX-M-166, a new CTX-M β-lactamase produced by ...
Manageiro, Vera; Graça, Rafael; Ferreira, Eugénia; Clemente, Lurdes; Bonnet, Richard; Caniça, ManuelaIn this study, we biochemically characterized the new CTX-M-166 β-lactamase detected in a ceftiofur-resistant Escherichia coli recovered in May 2014 from a six-week-old Gallus gallus broiler flock in an industrial poultry unit in the central region of Portugal.
New insights into resistance to colistin and third-generation cephalosporins of...
Manageiro, Vera; Clemente, Lurdes; Graça, Rafael; Correia, Ivone; Albuquerque, Teresa; Ferreira, Eugénia; Caniça, ManuelaThe increasing incidence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and Gram negative organisms that has been observed in food animals such as poultry, cattle and pigs, are suggestive that animals, food and environment are potential sources of ESBL-producing bacteria. Hence, the aim of this study was to characterized commensal E. coli obtained from healthy ...