6 documents found, page 1 of 1
Identification of Aspergillus cryptic species in hospital environment
Sabino, Raquel; Viegas, C.; Veríssimo, C.; Simões, H.; Brandão, João; Martins, C.; Clemons, K.V.; Stevens, D.A.Selected hospital wards, housing patients at higher risk to develop invasive fungal infections, were screened in order to understand the epidemiology and distribution of Aspergillus, especially regarding the presence of cryptic species.Aspergillus species were identified by b-tubulin and calmodulin sequencing, and a high percentage of cryptic species (i.e., not sensu stricto) was found (59%). Sections Usti, Ver...
Detection of cryptic species of Aspergillus with reduced susceptibility to anti...
Sabino, Raquel; Viegas, C.; Veríssimo, C.; Carolino, E.; Brandão, João; Simões, H.; Martins, C.; Clemons, K.V.; Stevens, D.A.Invasive aspergillosis is a fungal infection caused by Aspergillus spp. affecting mainly the immunocompromised. The mortality rate may reach 85%. Aspergillus identification should be based on molecular methods as there are species morphologically similar but distinct at the molecular level (cryptic species), with variable antifungal susceptibility profiles.
Molecular screening of 246 Portuguese Aspergillus isolates among different clin...
Sabino, R.; Veríssimo, C.; Parada, H.; Brandão, João; Viegas, C.; Carolino, E.; Clemons, K.V.; Stevens, D.A.Clinical and environmental samples from Portugal were screened for the presence of Aspergillus and the distribution of the different species-complexes determined to understand differences in their distribution from different sources. Fifty-seven Aspergillus isolates from clinical samples were collected from 10 health institutions. Six species-complexes were detected by ITS sequencing: Fumigati, Flavi, and Nigri...
Molecular Epidemiology of Aspergillus collected from Cystic Fibrosis Patients
Sabino, R.; Ferreira, J.A.G.; Moss, R.B.; Valente, J.; Verissimo, C.; Carolino, E.; Clemons, K.V.; Everson, C.; Banaei, N.; Penner, J.; Stevens, D.A.Background: Aspergillus respiratory infection is a common complication in cystic fibrosis (CF) and is associated with loss of pulmonary function and allergic disease. Methods: Fifty-three Aspergillus isolates recovered fromCF patients were identified to species by Internal Transcribed Spacer Region (ITS), β-tubulin, and calmodulin sequencing. Results: Three species complexes (Terrei, Nigri, and Fumigati) were f...
Screening of cryptic species among clinical Aspergillus isolates collected duri...
Sabino, R.; Veríssimo, C.; Parada, H.; Clemons, K.V.; Stevens, D.A.Objectives: Correct identification of Aspergillus species is important given that sibling species may show variable susceptibilities to multiple antifungal drugs and also because sharper definition of species may facilitate epidemiological studies. Thus, we screened Aspergillus clinical isolates from Portuguese hospitals to determine which, if any, of the cryptic species of Aspergillus were involved in patient ...
Surveillance of environmental fungi, with focus on Aspergillus, in a Portuguese...
Sabino, Raquel; Viegas, C.; Veríssimo, C.; Martins, C.; Clemons, K.V.; Stevens, D.A.Objectives: Because immunocompromised patients are more prone to acquire nosocomial infections caused by fungi isolated from the environment, e.g. Aspergillus, this study aimed to screen the hospital environment for the presence of fungi and to understand their epidemiology in the different hospital wards analyzed. Methods: During one-year period, four seasonal samplings, i.e., air and hard surface, were perfor...