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Comparison of Electrophoretic Protein Profiles from Sheep and Goat Parotid Saliva

Lamy, E.; da Costa, G.; Capela e Silva, Fernando; Potes, J.C.; Coelho, A.V.; Sales Baptista, E.

Saliva provides a medium for short-term adaptation to changes in diet composition, namely, the presence of plant secondary metabolites. Salivary proteins have biological functions that have particular influence on oral homeostasis, taste, and digestive function. Some salivary proteins, such as proline-rich proteins, are present in browsers but absent in grazers. Despite the significance of salivary proteins, th...


Salivary Amylase Induction by Tannin-Enriched Diets as a Possible Countermeasur...

da Costa, G.; Lamy, E.; Capela e Silva, Fernando; Andersen, J.; Sales Baptista, E.; Coelho, A.V.

Tannins are characterized by protein-binding affinity. They have astringent/bitter properties that act as deterrents, affecting diet selection. Two groups of salivary proteins, proline-rich proteins and histatins, are effective precipitators of tannin, decreasing levels of available tannins. The possibility of other salivary proteins having a co-adjuvant role on host defense mechanisms against tannins is unknow...


Utilização da proteómica para compreender o comportamento de ingestão

Lamy, E.; Capela e Silva, Fernando; Sales Baptista, E.; Graça, G.; da Costa, G.; Coelho, A.V.

O comportamento ingestivo é regulado, entre outros, por factores afectivos, os quais determinam diferentes preferências. A escolha da dieta depende de experiências anteriores, bem como do nível de percepção gustativa, estando a saliva envolvida neste último. As proteínas salivares desempenham papéis muito diversos que incluem a lubrificação, digestão, protecção contra microorganismos e factores antinutritivos, ...


Liquid chromatography–diode array detection–electrospray ionisation mass spectr...

Rauter, A. P.; Martins, A.; Borges, C.; Ferreira, J.; Justino, Jorge; Bronze, M.R.; Coelho, A.V.; Choi, Y.H.; Verpoorte, R.

The anti-hyperglycemic flavonoid extract obtained from Genista tenera was first studied by liquid chromatography (LC)–diode array detection (DAD) which showed the presence of two major compounds. One of them was identified as genistein-7-O-glucoside. Luteolin-7-O-glucoside was detected as a minor constituent, while luteolin-7,3′-di-O-glucoside and rutin were found in trace amounts. LC–DAD–ESI–MS and NMR were us...


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