2 documents found, page 1 of 1
Results of the 1999-2000 collaborative exercise and proficiency testing program...
Alonso, A; Salas, A; Albarrán, C; Arroyo, E; Castro, A; Crespillo, M; Lonardo, AM; Lareu, MV; Cubría, CL; Soto, ML; Lorente, JA; Semper, MM; Palacio, AThe Spanish and Portuguese working group (GEP) of international society for forensic genetics (ISFG) 1999-2000 collaborative exercise on mitochondrial DNA (mtDNA) included the analysis of four bloodstain samples and one hair shaft sample by 19 participating laboratories from Spain, Portugal and several Latin-American countries. A wide range of sequence results at position 16,093 of the HV1 (from T or C homoplas...
Failed PCR amplifications of MBP-STR alleles due to polymorphism in the primer ...
Gusmão, L; Amorim, A; Prata, MJ; Pereira, L; Lareu, MV; Carracedo, AThe PCR-based STR system MBP-B (myelin basic protein locus B) has been reported to exhibit a high rate of mutations. Using a newly designed pair of primers we present evidence that this is due to failed amplifications caused by a polymorphism in the annealing region of the reverse primer originally designed. With the new reverse primer described here no exclusions were found (out of 59 mother/child pairs analys...