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Adaptation and convergence in circadian‐related genes in Iberian freshwater fish

Moreno, João M; Jesus, Tiago F.; Coelho, Maria Manuela; Sousa, Vitor C

The circadian clock is a biological timing system that improves the ability of organisms to deal with environmental fluctuations. At the molecular level it consists of a network of transcription-translation feedback loops, involving genes that activate (bmal and clock - positive loop) and repress expression (cryptochrome (cry) and period (per) - negative loop). This is regulated by daily alternations of light b...


Protein analysis and gene expression indicate differential vulnerability of Ibe...

Jesus, Tiago Filipe; Moreno, João M.; Repolho, T.; Athanasiadis, Alekos; Rosa, Rui; Almeida-Val, Vera Maria Fonseca; Coelho, Maria Manuela

Current knowledge on the biological responses of freshwater fish under projected scenarios of climate change remains limited. Here, we examine differences in the protein configuration of two endemic Iberian freshwater fish species, Squalius carolitertii and the critically endangered S. torgalensis that inhabit in the Atlantic-type northern and in the Mediterranean-type southwestern regions, respectively. We per...

Date: 2020   |   Origin: Oasisbr

Allele-specific expression variation at different ploidy levels in Squalius alb...

Matos, Isa; Machado, Miguel P.; Schartl, Manfred; Coelho, Maria Manuela

Allopolyploid plants are long known to be subject to a homoeolog expression bias of varying degree. The same phenomenon was only much later suspected to occur also in animals based on studies of single selected genes in an allopolyploid vertebrate, the Iberian fish Squalius alburnoides. Consequently, this species became a good model for understanding the evolution of gene expression regulation in polyploid vert...


Transcriptome profiling of two Iberian freshwater fish exposed to thermal stress

Jesus, Tiago; Grosso, Ana Rita; Almeida-Val, Vera Maria Fonseca; Coelho, Maria Manuela

The congeneric freshwater fish Squalius carolitertii and S. torgalensis inhabit different Iberian regions with distinct climates; Atlantic in the North and Mediterranean in the South, respectively. While northern regions present mild temperatures, fish in southern regions often experience harsh temperatures and droughts. Previous work with two hsp70 genes suggested that S. torgalensis is better adapted to harsh...


Genomic Resources Notes Accepted 1 June 2015 - 31 July 2015

Álvarez, P.; Arthofer, Wolfgang; Coelho, Maria Manuela; Conklin, D.; Estonba, A.; Grosso, Ana Rita; Helyar, S. J.; Langa, J.; Machado, Miguel P.

This article documents the public availability of (i) microbiomes in diet and gut of larvae from the dipteran Dilophus febrilis using massive parallel sequencing, (ii) SNP and SSR discovery and characterization in the transcriptome of the Atlantic mackerel (Scomber scombrus, L) and (iii) assembled transcriptome for an endangered, endemic Iberian cyprinid fish (Squalius pyrenaicus).


Global analysis of the small RNA transcriptome in different ploidies and genomi...

Inácio, Angela; Pinho, Joana; Pereira, Patrícia Matos; Comai, Luca; Coelho, Maria Manuela

The Squalius alburnoides complex (Steindachner) is one of the most intricate hybrid polyploid systems known in vertebrates. In this complex, the constant switch of the genome composition in consecutive generations, very frequently involving a change on the ploidy level, promotes repetitive situations of potential genomic shock. Previously in this complex, it was showed that in response to the increase in genome...


Gene expression regulation and lineage evolution: the North and South tale of t...

Pala, Irene; Schartl, Manfred; Brito, Miguel; Malta-Vacas, Joana; Coelho, Maria Manuela

The evolution of hybrid polyploid vertebrates, their viability and their perpetuation over evolutionary time have always been questions of great interest. However, little is known about the impact of hybridization and polyploidization on the regulatory networks that guarantee the appropriate quantitative and qualitative gene expression programme. The Squalius alburnoides complex of hybrid fish is an attractive ...


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