33 documents found, page 1 of 4
Reliability of point-of-care circulating cathodic antigen assay for diagnosing ...
Favre, Tereza Cristina; Beck, Lilian Christina Nóbrega Holsback; Bezerra, Fernando Schemelzer Moraes; Graeff-Teixeira, Carlos; Coelho, Paulo Marcos ZechFundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Educação em Ambiente e Saúde. Rio de Janeiro, RJ, Brasil.; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Educação em Ambiente e Saúde. Rio de Janeiro, RJ, Brasil.; Universidade Federal do Ceará. Departamento de Análises Clínicas e Toxicológicas. Fortaleza, CE, Brasil.; Universidade Federal do Espírito Santo. Centro de Ciências da Saúd...
Low specificity of point-of-care circulating cathodic antigen (POC[sbnd]CCA) di...
Graeff-Teixeira, Carlos; Favero, Vivian; Pascoal, Vanessa Fey; Souza, Renata Perotto de; Rigo, Francine de Vargas; Agnese, Luis Hoffmann DallThis work was funded by the Secretaria de Vigil?ncia em Sa?de / Fundo Nacional de Sa?de / Minist?rio da Sa?de (SVS/FNS/MS) - [TED/FNS: 118/2017; SIAFI: 691919/25000.479741/2017?05].; Universidade Federal do Esp?rito Santo.Center for Health Sciences. Infectious Diseases Unit. Vit?ria, ES, Brazil / Pont?ficia Universidade Cat?lica do Rio Grande do Sul. School of Sciences. Research Group on Biomedical Parasitology...
Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni ?...
Magalh?es, Fernanda do Carmo; Resende, Samira Diniz; Senra, Carolina; Graeff-Teixeira, Carlos; Enk, Martin Johannes; Coelho, Paulo Marcos ZechUniversidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.; Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.; Funda??o Oswaldo Cruz. Instituto Ren? Rachou. Pesquisa Cl?nica e Pol?ticas P?blicas em Doen?as Infecciosas e Parasit?rias. Belo Horizonte, MG, Brazil....
IgE antibodies from schistosomiasis patients to recognize epitopes in potato ap...
Gusmão, Michélia Antônia do Nascimento; Emídio, Nayara Braga; Marconato, Danielle Gomes; Farani, Priscila Silva Grijó; Maniezzi, Luiz Felipe-; Introduction: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. Methods: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. Results: Most of the ...
Diagnosis of Schistosoma mansoni infections : what are the choices in Brazilian...
Moraes, Vanessa Silva; Shollenberger, Lisa Marie; Siqueira, Liliane Maria Vidal; Borges, William de Castro; Harn, Donald A.The population of Brazil is currently characterised by many individuals harbouring low-intensity Schistosoma mansoni infections. The Kato-Katz technique is the diagnostic method recommended by the World Health Organization (WHO) to assess these infections, but this method is not sensitive enough in the context of low egg excretion. In this regard, potential alternatives are being employed to overcome the limits...
Serological proteomic screening and evaluation of a recombinant egg antigen for...
Moraes, Vanessa Silva; Shollenberger, Lisa Marie; Borges, William de Castro; Rabello, Ana Lúcia Teles; Harn, Donald A.Background: Despite decades of use of control programs, schistosomiasis remains a global public health problem. To further reduce prevalence and intensity of infection, or to achieve the goal of elimination in low-endemic areas, there needs to be better diagnostic tools to detect low-intensity infections in low-endemic areas in Brazil. The rationale for development of new diagnostic tools is that the current st...
Schistosomiasis in the Amazon region: is the current diagnostic strategy still ...
Sousa, Sergei Rodrigo Magalh?es de; Carvalho, Alessandra de Queiroz; Cardoso, Joyce Favacho Nogueira; Coelho, Paulo Marcos Zech; Geiger, Stefan MichaelConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq). Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES). Instituto Evandro Chagas (IEC)/Secretaria de Vigil?ncia em Sa?de (SVS)/Minist?rio de Sa?de (MS); Universidade do Estado do Par?. Programa de P?s-Gradua??o Strictu Sensu em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em ...
Transmission control of schistosomiasis mansoni in a low endemicity area throug...
Carneiro, Nídia Francisca de Figueiredo; Caldeira, Antônio Prates; Katz, Naftale; Oliveira, Áureo Almeida de; Siqueira, Liliane Maria VidalA prospective cohort study with rigorous searching for schistosomiasis cases was conducted among residents of Pedra Preta, Montes Claros, Minas Gerais State, Brazil, seven years after an intervention. Kato-Katz (KK), Saline Gradient, Miracidia Hatch and Polymerase Chain Reaction (PCR) were used as the diagnostic methods in 2008. In the period of 2013-2016, 175 patients remaining in the area were examined using ...
Análise da resposta imune humoral humana a ATP difospomidrolase em pacientes co...
Vasconcelos, Eveline Gomes; Mendes, Rita Gabriela Pedrosa Ribeiro; Coelho, Paulo Marcos Zech; Porcino, Gabriane NascimentoIntrodução: A infecção por ancilostomídeos tem recebido atenção especial devido ao seu papel imunomodulador na esquistossomose, interferindo na susceptibilidade de indivíduos de área endêmica. A reatividade positiva entre a apirase de batata e anticorpos IgE ou IgG de pacientes com esquistossomose sugeriu que os domínios compartilhados entre as proteínas da batata e do parasito são antigênicos. Como as áreas ru...
Immunostimulatory property of a synthetic peptide belonging to the soluble ATP ...
Mendes, Rita Gabriela Pedrosa Ribeiro; Gusmão, Michélia Antônia do Nascimento; Maia, Ana Carolina Ribeiro Gomes; Detoni, Michelle de Lima-; A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In...