12 documents found, page 1 of 2
Reliability of point-of-care circulating cathodic antigen assay for diagnosing ...
Favre,Tereza Cristina; Beck,Lilian Christina Nóbrega Holsback; Bezerra,Fernando Schemelzer Moraes; Graeff-Teixeira,Carlos; Coelho,Paulo Marcos ZechABSTRACT Background The World Health Organization recommends reliable point-of-care (POC) diagnostic testing to eliminate schistosomiasis. Lateral flow immunoassay that detects schistosome circulating cathodic antigen (CCA) in urine to establish prevalence thresholds for intervention in endemic areas is recommended. Stored urine may be useful if surveying at-risk populations is delayed or interrupted by unfores...
Interaction of Biomphalaria cells in primary cultures with Schistosoma mansoni ...
Silva-Neto,Aristeu; Fonseca,Cristhiane Oliveira da; Silva,Luciana Maria; Coelho,Paulo Marcos Zech; Fortes-Dias,Consuelo LatorreAbstract INTRODUCTION: Biomphalaria snails may display varying levels of susceptibility to Schistosoma mansoni infection. We have been developing an in vitro model to study the interaction between the snail and the parasite, using tissue-derived cell cultures from Biomphalaria. METHODS: The digestive gland- and kidney-derived cells from primary cultures of resistant (B. tenagophila Taim) and susceptible (B. ten...
Development of a natural molluscicide prototype kit (MoluSchall) for the contro...
Paula-Andrade,Cynthia de; Coelho,Paulo Ricardo Silva; Nascimento,Ricardo Aurélio Pinto; Mota,Pedro Moacyr Pinto Coelho; Romano-Silva,Marco AurélioAbstract INTRODUCTION In Brazil, Biomphalaria glabrata, B. tenagophila, and B. straminea are intermediate hosts of Schistosoma mansoni, the etiological agent of schistosomiasis mansoni. Molluscicide use is recommended by the WHO for controlling the transmission of this parasite. Euphorbia milii latex has shown promising results as an alternative molluscicide. Thus, a natural molluscicide prototype kit based on ...
Performance of POC-CCA® in diagnosis of schistosomiasis mansoni in individuals ...
Siqueira,Liliane Maria Vidal; Couto,Flavia Fernanda Bubula; Taboada,Diana; Oliveira,Áureo Almeida de; Carneiro,Nidia Francisca de FigueiredoAbstract: INTRODUCTION: Schistosomiasis, caused by Schistosoma mansoni, is a public health concern in Brazil. However, the most popular diagnostic method, the Kato-Katz technique, exhibits low sensitivity in low-endemicity areas. We aimed to compare the performance of an immunological assay, the point-of-care circulating cathodic antigen (POC-CCA®) test, with that of two parasitological techniques in a low-ende...
Brain schistosomiasis in mice experimentally infected with Schistosoma mansoni
Lambertucci,José Roberto; Fidelis,Thiago André; Pereira,Thiago Almeida; Coelho,Paulo Marcos Zech; Araujo,Neuza; Souza,Márcia Maria deIntroduction Human neuroschistosomiasis has been reported in the literature, but the possibility of modeling neuroschistosomiasis in mice is controversial. Methods In two research laboratories in Brazil that maintain the Schistosoma mansoni life cycle in rodents, two mice developed signs of brain disease (hemiplegia and spinning), and both were autopsied. Results S. mansoni eggs, both with and without granuloma...
Acute schistosomiasis diagnosis: a new tool for the diagnosis of schistosomiasi...
Grenfell,Rafaella Fortini Queiroz; Martins,Watson; Drummond,Sandra Costa; Antunes,Carlos Maurício de Figueiredo; Voieta,Izabela; Otoni,AlbaIntroductionThe diagnosis of schistosomiasis mansoni on early stages of infection is important to prevent late morbidity. A simple, cheap, sensitive and specific assay for routine diagnosis of schistosome infection based on the detection of specific IgG for schistosomula tegument antigens (ELISA-SmTeg) was developed by our group.MethodsWe describe here an acute outbreak involving a travel group of 80 individual...
Antigens of worms and eggs showed a differentiated detection of specific IgG ac...
Grenfell,Rafaella Fortini Queiroz; Martins,Watson Hermann; Silva-Moraes,Vanessa; Barata,Suedali Villas-Boas; Ribeiro,Elizandra Giani; Oliveira,EdwardINTRODUCTION: The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease. METHODS: Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms soluble antigens and eggs soluble antigens were standardized and evaluated in our laboratory. Fifty mice were used in ...
Morbidity due to Schistosoma mansoni - Entamoeba histolytica coinfection in ham...
Dolabella,Silvio Santana; Coelho,Paulo Marcos Zech; Borçari,Izabela Torquetti; Mello,Nelson Azevedo Santos Teixeira; Andrade,Zilton de AraújoData on Schistosoma mansoni-Entamoeba histolytica coinfection are scarce in the literature. In the present study, hamsters that had been infected for 70 days with Schistosoma mansoni (LE strain) were inoculated via the portal vein with two strains of trophozoites of Entamoeba histolytica: ICB-EGG (highly virulent) and ICB-RPS (non-virulent). The most evident result of coinfection was increased morbidity and mor...
Antischistosomal activity of acridanone- hydrazones in Cebus monkeys experiment...
Coelho,Paulo Marcos Zech; Pereira,Leógenes Horácio; Mello,Rômulo Teixeira deIn this study, four compounds were utilized at the dose of 12.5mg/kg body weight, p.o., to treat Cebus monkeys experimentally infected with about 200 cercariae of Schistosoma mansoni (SJ strain), via transcutaneous route. The oograms performed with rectal snips, as well as stool examinations carried out periodically, showed no viable eggs of the parasite, from day 29 to 226post-treatment. The perfusion undertak...
Schistosoma mansoni: on the possibility of Indian buffalo (Bubalus bubalis) bei...
Coelho,Paulo Marcos Zech; Lima,Walter S.; Nogueira,Raimundo H. G.Male Indian buffalo (Bubalus bubalis) calves were submitted to Schistosoma mansoni infection by percutaneous, oral and subcutaneous routes. No worms or eggs were found in four of the animals tested. Bubalus bubalis appears to be refractory for S. mansoni.