11 documents found, page 1 of 2
Correlation study and histopathological description of intestinal alterations i...
Silva,Diogo Tiago; Neves,Maria Francisca; Queiroz,Nina Mari Gual Pimenta de; Spada,Julio Cesar Pereira; Alves,Maria Luana; Flóro e Silva,MarinaAbstract The aim of this work was a correlation study and histopathological description of alterations associated with the presence of Leishmania infantumamastigote in the intestinal wall of dogs infected with canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally infected dogs with CVL with amastigotes of L. infantum in the small and large intestines; G2 (n = 9), infected...
Comparative study of five techniques for the diagnosis of canine gastrointestin...
Coelho,Willian Marinho Dourado; Gomes,Jancarlo Ferreira; Falcão,Alexandre Xavier; Santos,Bianca Martins dos; Soares,Felipe AugustoDifferences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TFGII/Dog). Fifty naturally infected dogs were housed in individual stalls, and their feces were evaluated comparati...
A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs
Coelho,Willian Marinho Dourado; Gomes,Jancarlo Ferreira; Amarante,Alessandro Francisco Talamini do; Bresciani,Katia Denise Saraiva; Lumina,GiulianoIn this study, we aimed to introduce a new technique calledTF-Test Modified∕Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified∕Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher i...
Molecular and parasitological detection of Leishmania spp. in a dipteran of the...
Coelho,Willian Marinho Dourado; Bresciani,Katia Denise SaraivaLeishmaniasis is an important chronic zoonosis caused by protozoa of the genus Leishmania spp. The major vectors of this protozoosis are sand flies, and Lutzomyia longipalpis is considered the main species implicated in the transmission of American Visceral Leishmaniasis in Brazil. The presence of the parasite's deoxyribonucleic acid (DNA) in ectoparasites such as ticks and fleas has prompted speculations about...
Occurrence of gastrointestinal parasites in goat kids
Coelho,Willian Marinho Dourado; Amarante,Alessandro Francisco Talamini do; Bresciani,Katia Denise SaraivaFecal samples from male and female goat kids, of different breeds and up to one year of age, were analyzed to determine egg and oocyst counts per gram of feces (EPG and OPG, respectively), and fecal culturing was performed to identify nematode genera. Helminth eggs and Eimeria spp. oocysts were found in 93.06% (188/202) and 77.22% (156/202) of the fecal samples, respectively. From fecal cultures, the following ...
Cryptosporidium spp. infection in mares and foals of the northwest region of Sã...
Inácio,Sandra Valéria; Brito,Roberta Lomonte Lemos de; Zucatto,Anaiza Simão; Coelho,Willian Marinho Dourado; Aquino,Monally Conceição Costa deThe present study aimed to analyze the occurrence of infection by Cryptosporidium spp. in mares and their respective foals. This study was carried out in 11 farms located in the municipalities of Araçatuba, Birigui, Guararapes and Santo Antônio do Aracangua, in the northwest region of the State of Sao Paulo, from November 2010 to March 2011. A total of 98 mares and 98 foals of several breeds were analyzed; amon...
Análise da influência do ultrassom de baixa intensidade na região de reparo óss...
Apolinário,Juliana de Carvalho; Coelho,Willian Marinho Dourado; Louzada,Mário Jefferson QuirinoHá evidências de que o ultrassom (US) de baixa intensidade pode acelerar a regeneração óssea. Este trabalho objetivou verificar a ação do US no defeito ósseo, criado experimentalmente em tíbias de ratos sob ausência de carga. Vinte Rattus novergicus albinus, Wistar adultos, divididos em: G1 (n=10), grupo experimental de 15 dias sem suspensão, e G2 (n=10), grupo experimental de 15 dias suspenso pela cauda, foram...
Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct imm...
Teixeira,Weslen Fabricio Pires; Coelho,Willian Marinho Dourado; Nunes,Cáris Maroni; Meireles,Marcelo VasconcelosThe aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIF) for detecting C. parvum oocysts in fecal samples from calves. In order to obtain anti-C. parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund's adjuvant. Purification of the i...
Ocorrência de Leishmania spp. em felinos do município de Araçatuba, SP
Bresciani,Katia Denise Saraiva; Serrano,Ana Claudia Marques; Matos,Lucas Vinicius Shigaki de; Savani,Elisa San Martin MourizEste trabalho teve como objetivo comparar a ocorrência de Leishmania spp. em gatos por dois métodos (citológico e sorológico), bem como associar a ocorrência deste protozoário com as variáveis sexo, idade e raça. Amostras séricas de 283 felinos domésticos foram testadas pela Reação de Imunofluorescência Indireta (RIFI), e o exame parasitológico direto de linfonodos também foi realizado para a verificação da pos...
Occurrence of Leishmania (Leishmania) chagasi in a domestic cat (Felis catus) i...
Coelho,Willian Marinho Dourado; Lima,Valéria Marçal Felix de; Amarante,Alessandro Francisco Talamini do; Langoni,Helio; Pereira,Virgínia Bodelão RichiniThis work describes natural infection by Leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. Positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA), respectively. Polymerase chain reaction (PCR) revealed that the nucleotide sequence of the sample was iden...