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The Use of Simulators in Training for Bovine Reproductive Procedures: A Scoping...

Azuaga Filho, Heitor; Colaço, Bruno; Payan-Carreira, Rita

Training in bovine reproduction requires not only technical proficiency but also ethical responsibility and adherence to animal-welfare standards. Traditional instruction relies heavily on repeated practice in abattoir-collected specimens and live cattle, raising concerns about stress, variability, logistical constraints, and student anxiety. Simulation-based education (SBE) has therefore emerged as a pedagogic...


Exploring Morphometric Features of the Female Reproductive Tract in Brazilian P...

Azuaga-Filho, Heitor; Payan-Carreira, Rita; Colaço, Bruno

The cattle in the Pantanal region show a notable influence from Bos indicus breeds and their crossbreeds. However, a comprehensive biometric assessment of the reproductive system in these animals is currently lacking. This study evaluated the effects of breed, age, carcass weight, and estrous cycle phase on female reproductive system morphometry. A total of 124 healthy, non-pregnant reproductive tracts (83 Nelo...


Development of a New Simulator for Training Nelore Artificial Insemination

Azuaga Filho, Heitor; Colaço, Bruno; Payan-Carreira, Rita

Background Regular training is crucial for student and novice operators conducting artificial insemination to ensure successful cattle reproduction. Standardized repetitions help generate autonomy and self-confidence. It is also essential for proficiency in the technique, assuring consistency and success in the procedure. Training live animals for AI procedures raises ethical concerns and requires a regular sup...


Comprehensive Validation of the TrAI4Nel Simulator for Nelore Artificial Insemi...

Azuaga-Filho, Heitor; Santos, Alexandre; Colaço, Bruno; Payan Carreira, Rita

Effective bovine artificial insemination (AI) training requires balancing technical skill development with animal welfare considerations. Commercial simulators typically replicate Bos taurus anatomy, limiting utility in regions where Bos indicus breeds predominate. This study validates the TrAI4Nel simulator, customized for Nelore cattle AI training. Validation employed a multi-dimensional framework encompassin...


Validation of the rat model of prostate cancer: correlating seminal vesicle les...

Nascimento-Gonçalves, Elisabete; Seixas, Fernanda; Oliveira, Bruno MM; Teiga-Teixeira, João; Faustino-Rocha, Ana I; Colaço, Bruno; Ferreira, Rita

Background/aim: Lesions in the seminal vesicle are described in the most used protocols for prostate cancer (PCa) induction. This study aimed to characterize the lesions of seminal vesicles associated with a protocol of PCa induction in rats to contribute to better characterization of this model. Materials and methods: Forty-five male Wistar Unilever rats were randomly divided into two control groups: CONT1 (n=...


The History and Prospects of Rabbit Sperm Sexing

Pinto-Pinho, Patrícia; Ferreira, Ana F.; Pinto-Leite, Rosário; Fardilha, Margarida; Colaço, Bruno

Sperm sex selection is a longstanding challenge in the field of animal reproduction. The cuniculture industry, in particular producers of males or females for breeding purposes, would greatly benefit from the pre-selection of the offspring's sex. This review article overviews the current and future developments in rabbit sperm sexing technologies, as well as the implications of implementing these methodologies ...


Sustainable animal production: exploring the benefits of sperm sexing technolog...

Quelhas, Joana; Pinto-Pinho, Patrícia; Lopes, Graça; Rocha, António; Pinto-Leite, Rosário; Fardilha, Margarida; Colaço, Bruno

The sex of the animals is of paramount importance in many animal production systems. This is particularly evident in the production of milk or in breeding programs focused on the production of female animals. In some cases, slaughter or euthanasia of animals of the unwanted sex becomes the only solution, highlighting ethical and economic concerns. As global demand for food continues to rise, the importance of a...


Lifelong exercise training promotes the remodelling of the immune system and pr...

Nascimento-Gonçalves, Elisabete; Seixas, Fernanda; Palmeira, Carlos; Martins, Gabriela; Fonseca, Carolina; Duarte, José Alberto; Faustino-Rocha, Ana I

This work aimed to understand how lifelong exercise training promotes the remodelling of the immune system and prostate signalome in a rat model of PCa. Fifty-five male Wistar rats were divided into four groups: control sedentary, control exercised, induced PCa sedentary and induced PCa exercised. Exercised animals were trained in a treadmill for 53 weeks. Pca induction consisted on the sequential administratio...


Pregnancy Complications and Feto-Maternal Monitoring in Rabbits

Pinto-Pinho, Patrícia; Pinto, Maria de Lurdes; Monteiro, José; Fardilha, Margarida; Pinto-Leite, Rosário; Colaço, Bruno

Rabbit production holds significant relevance in modern agriculture due to its potential as a sustainable source of high-quality protein and efficient feed conversion, contributing to food security and economic diversification. Nevertheless, studies incorporating feto-maternal monitoring in this species are uncommon. This review gathers research on the monitoring and evaluation of factors affecting rabbit gesta...


The influence of physical exercise on oestrogen and androgen receptor expressio...

Nascimento-Gonçalves, Elisabete; Seixas, Fernanda; Faustino-Rocha, Ana Isabel; Pires, Maria João; Neuparth, Maria João; Colaço, Bruno; Ferreira, Rita

Background: Oestrogen (ER) and androgen (AR) recep- tors play an important role in normal prostate development and are also implied in prostate cancer (PCa) development. Several studies suggested that physical activity may decrease the risk of PCa development and also changes sexual hor- mones and their receptors. This study aimed to evaluate the effects of physical exercise on ERα and AR expression in a rat mo...


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