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Molecular identification of clinical and environmental avian Aspergillus isolates

Sabino, Raquel; Burco, Julia; Valente, Joana; Veríssimo, Cristina; Clemons, Karl V.; Stevens, David A.; Tell, Lisa A.

Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period, clinical samples were recovered from 44 birds with clinical signs of the disease, clinical pa...


The role of occupational Aspergillus exposure in the development of diseases

Sabino, Raquel; Veríssimo, Cristina; Viegas, Carla; Viegas, Susana; Brandão, João; Alves-Correia, Magna; Borrego, Luís-Miguel; Clemons, Karl V.

Aspergillus spp. have a high nutritional versatility and good growth on a large variety of construction materials. They also colonize soil or food, but decaying vegetation is their primary ecological niche. Therefore, exposure to fungi may occur at home, during hospitalization, during specific leisure activities, or at the workplace. The development of Aspergillus infections depends on the interplay between hos...


The role of occupational Aspergillus exposure in the development of diseases

Sabino, Raquel Filipa Pinheiro; Veríssimo, Cristina; Viegas, Carla; Viegas, Susana; Brandão, João; Correia, Magna Alves; Borrego, Luís Miguel

Aspergillus spp. have a high nutritional versatility and good growth on a large variety of construction materials. They also colonize soil or food, but decaying vegetation is their primary ecological niche. Therefore, exposure to fungi may occur at home, during hospitalization, during specific leisure activities, or at the workplace. The development of Aspergillus infections depends on the interplay between hos...


Antifungal susceptibility of 175 Aspergillus isolates from various clinical and...

Sabino, Raquel; Carolino, Elisabete; Veríssimo, Cristina; Martinez, Marife; Clemons, Karl V.; Stevens, David A.

Some environmental Aspergillus spp. isolates have been described as resistant to antifungals, potentially causing an emerging medical problem. In the present work, the antifungal susceptibility profile of 41 clinical and 134 environmental isolates of Aspergillus was determined using the CLSI microdilution method. The aim of this study was to compare environmental and clinical isolates with respect to their susc...


Antifungal susceptibility of 175 Aspergillus isolates from various clinical and...

Sabino, Raquel; Carolino, Elisabete; Veríssimo, Cristina; Martinez, Marife; Clemons, Karl V.; Stevens, David A.

Some environmental Aspergillus spp. isolates have been described as resistant to antifungals, potentially causing an emerging medical problem. In the present work, the antifungal susceptibility profile of 41 clinical and 134 environmental isolates of Aspergillus was determined using the CLSI microdilution method. The aim of this study was to compare environmental and clinical isolates with respect to their susc...


Inhibition of Aspergillus fumigatus and its biofilm by Pseudomonas aeruginosa i...

Ferreira, José A. G.; Penner, John C.; Moss, Richard B.; Haagensen, Janus A.; Clemons, Karl V.; Spormann, Alfred M.; Nazik, Hasan; Cohen, Kevin

Aspergillus fumigatus (Af) and Pseudomonas aeruginosa (Pa) are leading fungal and bacterial pathogens, respectively, in many clinical situations. Relevant to this, their interface and co-existence has been studied. In some experiments in vitro, Pa products have been defined that are inhibitory to Af. In some clinical situations, both can be biofilm producers, and biofilm could alter their physiology and affect ...


Molecular epidemiology of Aspergillus collected from cystic fibrosis patients

Sabino, Raquel; Ferreira, Jose A.G.; Moss, Richard B.; Valente, Joana; Veríssimo, Cristina; Carolino, Elisabete; Clemons, Karl V.; Everson, Cassie

Background - Aspergillus respiratory infection is a common complication in cystic fibrosis (CF) and is associated with loss of pulmonary function and allergic disease. Methods - Fifty-three Aspergillus isolates recovered from CF patients were identified to species by Internal Transcribed Spacer Region (ITS), β-tubulin, and calmodulin sequencing. Results - Three species complexes (Terrei, Nigri, and Fumigati) we...


Detection of cryptic species of Aspergillus with reduced susceptibility to anti...

Sabino, Raquel; Viegas, Carla; Veríssimo, Cristina; Carolino, Elisabete; Brandão, João; Parada, Helena; Martins, Carlos; Clemons, Karl V.

Invasive aspergillosis is a fungal infection caused by Aspergillus spp. affecting mainly the immunocompromised. The mortality rate may reach 85%. Aspergillus identification should be based on molecular methods as there are species morphologically similar but distinct at the molecular level (cryptic species), with variable antifungal susceptibility profiles.


Molecular screening of 246 Portuguese Aspergillus isolates among different clin...

Sabino, Raquel; Veríssimo, Cristina; Parada, Helena; Brandão, João; Viegas, Carla; Carolino, Elisabete; Clemons, Karl V.; Stevens, David A.

Clinical and environmental samples from Portugal were screened for the presence of Aspergillus and the distributions of the species complexes were determined in order to understand how their distributions differ based on their source. Fifty-seven Aspergillus isolates from clinical samples were collected from 10 health institutions. Six species complexes were detected by internal transcribed spacer sequencing; F...


Aspergillus em ambiente hospitalar: um risco para o desenvolvimento de infeções...

Raquel, Sabino; Veríssimo, Cristina; Viegas, Carla; Brandão, João; Parada, Helena; Martins, Carlos; Furtado, Cristina; Clemons, Karl V.


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