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Inter-laboratory variation in measurement of DNA damage by the alkaline comet a...

Moller, P; Azqueta, A; Collia, M; Bakuradze, T; Richling, E; Bankoglu, EE; Stopper, H; Bastos, VC; Langie, SAS; Jensen, A; Ristori, S; Scavone, F

The comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of DNA damage. Certain steps in the comet assay procedure have substantial impact on the magnitude of DNA migration (e.g. electric potential and time of electrophoresis). Inter-l...

Date: 2023   |   Origin: Repositório Aberto da Universidade do Porto
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