36 documents found, page 1 of 4
Simplified, high yielding extraction of xylan/xylo-oligosaccharides from palmar...
Coelho, Diogo; Costa, Diogo Félix; Barroca, Mário; Cunha, Sara Alexandra; Pintado, Maria Manuela; Abreu, Helena; Martins, Margarida; Collins, TonyThe complex plant cell wall heteropolysaccharide xylan, and its breakdown products xylo-oligosaccharides and xylose, are value-added compounds with a plethora of potential applications in diverse areas. They are nonetheless currently poorly exploited, with a major bottleneck being the unavailability of efficient, low-cost, high-yield production processes. The major objective of the present study is to identify ...
Candida albicans chitinase 3 with potential as a vaccine antigen: production, p...
Barbosa, Augusto Alexandre Costa; Ferreira, Diogo; Pacheco, Maria Inês; Casal, Margarida; Duarte, Henrique Oliveira; Gomes, CatarinaChitinases are widely studied enzymes that have already found widespread application. Their continued development and valorisation will be driven by the identification of new and improved variants and/or novel applications bringing benefits to industry and society. We previously identified a novel application for chitinases wherein the Candida albicans cell wall surface chitinase 3 (Cht3) was shown to have pote...
Design of a lipid nano-delivery system containing recombinant Candida albicans ...
Costa-Barbosa, Augusto; Pacheco, Maria Inês; Carneiro, Catarina; Botelho, Cláudia M.; Gomes, Andreia C; Real Oliveira, M. Elisabete C.D.; Collins, TonyOpportunistic fungi cause lethal systemic infections and impose high medical costs to health systems. The World Health Organization has recognized the importance of fungal infections, including them in its global priority list guiding research, development, and discovery of new therapeutic approaches. Fungal vaccine development has been proposed as one of the treatment and prevention strategies in the last deca...
Development and Characterization of Monoolein-Based Liposomes of Carvacrol, Cin...
Miranda-Cadena, Katherine; Dias, Marisol; Costa-Barbosa, Augusto; Collins, Tony; Marcos-Arias, Cristina; Eraso, Elena; Pais, Célia; Quindos, GuillermoVolume 65, no. 4, e01628-20, 2021, https://doi.org/10.1128/AAC.01628-20. In the original published version of this paper, posted on 18 March 2021, incorrect files were used for Fig. 6 to 10. In addition, “P, 0.5” should be “P, 0.05” in the legends of Fig. 7, 8, and 10, and in the 5th paragraph of the Results section, “64” in the 2nd-to-last sentence should be “32” and “P, 0.5“in the last sentence should be “P, ...
Development and characterization of monoolein-based liposomes of carvacrol, cin...
Miranda-Cadena, Katherine; Dias, Marisol; Costa-Barbosa, Augusto; Collins, Tony; Marcos-Arias, Cristina; Eraso, Elena; Pais, Célia; Quindos, GuillermoThere is an increasing need for novel drugs and new strategies for the therapy of invasive candidiasis. This study aimed to develop and characterize liposome-based nanoparticles of carvacrol, cinnamaldehyde, citral, and thymol with anti-Candida activities. Dioctadecyldimethylammonium bromide- and monoolein-based liposomes in a 1:2 molar ratio were prepared using a lipid-film hydration method. Liposomes were ass...
Microbial ecology of the cryosphere (glacial and permafrost habitats): current ...
Margesin, Rosa; Collins, TonyMicroorganisms in cold ecosystems play a key ecological role in their natural habitats. Since these ecosystems are especially sensitive to climate changes, as indicated by the worldwide retreat of glaciers and ice sheets as well as permafrost thawing, an understanding of the role and potential of microbial life in these habitats has become crucial. Emerging technologies have added significantly to our knowledge...
Psychrophilic lifestyles: mechanisms of adaptation and biotechnological tools
Collins, Tony; Margesin, RosaCold-adapted microorganisms inhabiting permanently low-temperature environments were initially just a biological curiosity but have emerged as rich sources of numerous valuable tools for application in a broad spectrum of innovative technologies. To overcome the multiple challenges inherent to life in their cold habitats, these microorganisms have developed a diverse array of highly sophisticated synergistic ad...
Conditions promoting effective very high gravity sugarcane juice fermentation
Monteiro, Bruno; Ferraz, Pedro; Barroca, Mário Jorge Faria; Cruz, Sandra H. da; Collins, Tony; Lucas, CândidaAbstract Background: Applying very high gravity (VHG) fermentation conditions to the sugarcane juice (SCJ) bioethanol industry would improve its environmental and economic sustainability without the need for major infrastructure changes or investments. It could enable a decrease in the consumption of biological and natural resources (cane/ land, water and energy) while maintaining acceptable production paramete...
Quantitative assessment of DNA damage in the industrial ethanol production stra...
Silva, Paulo César; Domingues, Lucília; Collins, Tony; Oliveira, Rui Pedro Soares de; Johansson, BjörnLignocellulosic hydrolysates remain one of the most abundant substrates for the sustainable production of second generation fuels and chemicals with Saccharomyces cerevisiae. Fermentation inhibitors such as acetic acid, furfural and hydroxymethylfurfural are formed in varying amounts depending of the hydrolysis conditions which cause slow or stuck fermentations, a topic that has garnered much research. Some fer...
Quantitative assessment of DNA damage in the industrial ethanol production stra...
Silva, Paulo César; Domingues, Lucília; Collins, Tony; Oliveira, Rui Pedro Soares de; Johansson, BjörnLignocellulosic hydrolysates remain one of the most abundantly used substrates for the sustainable production of second generation fuels and chemicals with Saccharomyces cerevisiae. Nevertheless, fermentation inhibitors such as acetic acid, furfural and hydroxymethylfurfural are formed during the process and can lead to slow or stuck fermentations and/or act as genotoxic agents leading to production strain gene...