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Effect of Xylo-oligosaccharides and cello-oligosaccharides obtained from Agro-i...

Silva, Marcos Fellipe da; Batista, José Josenildo; Colunga, Alondra; Pereira, Catarina Rodrigues; Teixeira, J. A.; Nobre, Clarisse; Goldbeck, Rosana

Agro-industrial residues, traditionally considered low-value by-products, have shown great potential to produce prebiotic oligosaccharides, such as xylo-oligosaccharides (XOS) and cello-oligosaccharides (COS). This study investigated the effects of XOS and COS, derived from sugarcane bagasse (SB) and corn husks (CH) by enzymatic hydrolysis, on the modulation of human intestinal microbiota in vitro. Cellulose an...


Effect of xylo-oligosaccharides and cello-oligosaccharides obtained from agro-i...

Silva, Marcos Fellipe da; Colunga, Alondra; Batista, José Josenildo; Pereira, Catarina; Nobre, Clarisse; Teixeira, J. A.; Coelho, Rosana Goldbeck

Agro-industrial residues, typically viewed as a low-value by-product, have demonstrated significant potential to produce prebiotic oligosaccharides, such as xylo-oligosaccharides (XOS) and cello-oligosaccharides (COS). This study evaluates the effects of XOS and COS, derived from sugarcane bagasse (SB) and corn hulls (CH) through enzymatic hydrolysis, on the modulation of human intestinal microbiota in vitro. S...


In vitro models as a tool to study the role of gut microbiota in obesity

Colunga, Alondra; Conceição, Eva Martins; Teixeira, J. A.; Nobre, Clarisse

Obesity, a highly prevalent condition worldwide that leads to the development of multiple metabolic diseases, has been related to gut microbial dysbiosis. To understand this correlation, in vivo models have been extremely useful. However, its use is limited by associated ethical concerns, high costs, low representativeness, and low reproducibility. Therefore, new and improved in vitro models have been developed...


OC33 Chromatographic approaches to study pine nut skin: exploitation of its com...

Silva, Soraia P.; Colunga, Alondra; Roupar, Dalila; Salvador, Andreia Filipa Ferreira; Nobre, Clarisse; Reis, S. F.; Freitas, V.; Coimbra, M. A.

Pine nut skin (PNS) is an unexploited and uncharacterized by-product recovered during pine nut processing. The exploitation of by-products as sources of valuable compounds agrees with the current demand for the reduction of waste, and a transition to more sustainable production and consumption1. Therefore, PNS characterization and bioactive potentialities were assessed. The utilization of several chromatographi...


Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in...

Nobre, Clarisse; Colunga, Alondra; Losoya, Carolina; Teixeira, J. A.; Belmares, Ruth; Abrunhosa, Luís

Powdered Pleurotusostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, whereas ZEN was adsorbed by PO. OTA was totally eliminated at pH 5 and ...


Production of a protein hydrolysate of Pleurotus ostreatus mushroom

Colunga, Alondra; Cruz-Hernandez, M.; Losoya, Carolina; Loredo, Araceli; Rodríguez-Jasso, Rosa María; Contreras, Juan; Gonçalves, Clarisse Salomé Nobre

Fast demographic growth has led to a growing interest in low-cost alternative protein sources to meet demands. Consequently, the attention of many researchers has focused on finding under- exploited sources of proteins, alternatives to those of animal origin, usually plant proteins have been used for this purpose. However, fungal proteins can be of a higher quality than those from many plants and may have certa...


Effect of Pleurotus ostreatus powder on the nutritional characteristics of bread

Losoya, Carolina; Cruz-Hernandez, M.; Colunga, Alondra; Rodríguez-Jasso, Rosa María; Loredo, Araceli; Flores, Adriana; Gonçalves, Clarisse Salomé Nobre

Pleurotus ostreatus is one of the most produced edible mushrooms worldwide. It is a source of high quality proteins, rich in fiber, vitamins, minerals and essential amino acids. Several bioactive compounds have been identified in P. ostreatus, such as -glucans, with immunosuppressive and immunomodulatory activities, hypoglycemic and prebiotic effects. Aguamiel, obtained from Agave Salmiana, is a sweet-tasting l...


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