3 documents found, page 1 of 1
The membrane periodic skeleton is an actomyosin network that regulates axonal d...
Costa, AR; Sousa, SC; Pinto-Costa, R; Mateus, JC; Lopes, CDF; Costa, AC; Rosa, D; Machado, D; Pajuelo, L; Wang, X; Zhou, FQ; Pereira, AJ; Sampaio, PNeurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain-and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active...
Collar occupancy: a new quantitative imaging tool for morphometric analysis of ...
Nova, FB; Maia, AF; Cruz, A; Millar, V; Pinto, IM; Relvas, JB; Domingues, HS"Background Oligodendrocytes (OL) are the myelinating cells of the central nervous system. OL differentiation from oligodendrocyte progenitor cells (OPC) is accompanied by characteristic stereotypical morphological changes. Quantitative imaging of those morphological alterations during OPC differentiation is commonly used for characterization of new molecules in cell differentiation and myelination and screenin...
Robust gap repair in the contractile ring ensures timely completion of cytokine...
Silva, AM; Osório, DS; Pereira, AJ; Maiato, H; Pinto, IM; Rubinstein, B; Gassmann, R; Telley, IA; Carvalho, AXCytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to sev...