55 documents found, page 1 of 6
Automated manufacturing of three-dimensional cell matrices with nanofibres of c...
Completo, A.; Marques, P. A. A. P.This invention relates to a system and a process for the automated manufacture of three-dimensional cell matrices by electrospinning from nanofibres of controlled alignment and uniform cell distribution throughout their thickness. From the implementation of the invention it is possible to obtain, in a totally automatic way and without manual intervention, three-dimensional cell matrices of aligned polymeric fib...
Development of a DoE with a new electrospinning system for cartilage tissue eng...
Silva, E.; Semitela, A.; Marques, P. A. A. P.; Completo, A.Electrospinning is currently one of the most used techniques to produce fibrous synthetic tissues such as cartilage and bone. To replicate cartilage tissue engineering functionality, one of the most important characteristics is the alignment of the resulting fibre meshes in a three-dimensional (3D) fashion. Here, a newly developed electrospinning collector system is tested in order to understand how the process...
Automated fabrication of 3D chondrocyte-laden anisotropic scaffolds for articul...
Semitela, Â.; Pereira, A. L.; Capitão, A.; Mendes, A. F.; Marques, P. A. A. P.; Completo, A.Tissue engineering (TE) strategies for repairing and regenerating articular car-tilage face critical challenges to approximate the biochemical and biomechanical microen-vironment of native tissue, particularly regarding collagen fibril depth-orientation and chondrocyte distribution. Here, a recently developed electromechanically 3D electrospin-ning platform was employed to develop three-dimensional (3D) anisotr...
Bilayered arcade-like scaffolds for articular cartilage repair
Semitela, Â.; Couto, J.; Capitão, A.; Mendes, A. F.; Marques, P. A. A. P; Completo, A.Articular cartilage is a highly organized tissue that it is adapted to the complex mechanical loading in joints. Given the limited self-healing of this tissue, tissue engineering (TE) strategies have explored the development of 3D anisotropic fibrous scaffolds with the implementation of specific mechanical stimulus. However, this functionally is dependent on the ability to recreate the depth-dependent collagen ...
Multi–layered electrospinning and electrospraying approach: effect of polymeric...
Semitela, A.; Pereira, A.; Sousa, C.; Mendes, A. F.; Marques, P. A. A. P.; Completo, A.Articular cartilage was expected to be one of the first tissues to be successfully engineered, but replicating the complex fibril architecture and the cellular distribution of the native cartilage has proven difficult. While electrospinning has been widely used to reproduce the depth–dependent fibre architecture in 3D scaffolds, the chondrocyte-controlled distribution remains an unsolved problem. To incorporate...
Femoral revision knee Arthroplasty with Metaphyseal sleeves: the use of a stem ...
Fonseca, F.; Sousa, A.; Completo, A.Purpose Metaphyseal sleeves are an option for patients with severe metaphyseal bony defects requiring TKA revision. Although sleeves are usually used with stems, little is known about the exact contribution/need of the stem for the initial sleeve-bone interface stability, particularly in the femur, if the intramedullary canal is deformed or bowed. It is hypothesised that diaphyseal-stem addition increases the s...
Fabricação automática de matrizes celulares tridimensionais com nanofibras de a...
Completo, A.; Marques, P. A. A. P.A presente invenção diz respeito a sistema e processo de fabrico automático de matrizes celulares tridimensionais com nanofibras de alinhamento controlado e distribuição celular uniforme ao longo da espessura. O sistema da presente invenção compreende um módulo de formação de nanofibras por eletrofiação, um modulo coletor das nanofibras constituído por dois cilindros coletores (6,30), um módulo de deposição das...
Electrospinning system and process for large scale manufacturing of aligned 3D ...
Completo, A.; Marques, P. A. A. P.This invention relates to the system and process of continuous electrospinning for the production of three-dimensional matrices of aligned polymeric fibres. The system of the present invention comprises an electrospinning capillary tube (3) with positive polarity, a set of multi-electrodes with negative polarity inserted in a peripheral support (15), having each electrode (7) controlled movement allowing its ex...
Influence of porosity and cell density on tissue engineering of mandibular cond...
Cortez, Sara Isabel Couto; Alves, J. L.; Completo, A.One of the major problems of the cartilage tissue engineering is to produce materials with similar biomechanical properties to those in the native tissue. Some parameters of the solid matrix such as the porosity of the scaffold and the number of cells may limit physiologically the nutrient supply and tissue growth. This work aims to simulate the impact of the porosity and the initial cell seeding in condylar ch...
Sistema e processo de fabricação em larga escala de matrizes tridimensionais de...
Completo, A.; Marques, P. A. A. P.A presente invenção diz respeito a sistema e processo de eletrofiação em contínuo para produção de matrizes tridimensionais de fibras poliméricas alinhadas. O sistema da presente invenção compreende um tubo capilar de eletrofiação (3) com polaridade positiva, um conjunto de multi elétrodos com polaridade negativa inseridos num suporte periférico (15), tendo cada elétrodo (7) movimento controlado permitindo a su...