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Alternative chemo-enzymatic hydrolysis strategy applied to different microalgae...

Constantino, Ana; Rodrigues, Brígida; Leon, R.; Barros, Raúl; Raposo, Sara

Microalgae have been considered third generation feedstock for biofuel production based on the expectation that large amounts of algal biomass can be cultivated at an acceptable cost. Transformation of biomass into ethanol requires a saccharification step, where complex carbohydrates are broken down by hydrolysis into sugars that can be fermented to bioethanol. Carbohydrate mobilization is hampered by the recal...


Chemical and physical pretreatments of microalgal biomass

Rodrigues, Brígida; Borges, Rodrigo; Castro, Maria; Constantino, Ana; Raposo, Sara

Non-axenic microalga Chlorella sorokiniana was cultivated in batch cultures, and its total sugar composition was determined. The microalga under study showed a total sugar concentration of 21.44 ± 0.46% (w/w). The effects of freeze-drying, oven-drying, freezing and thawing, chemical and the combination of hydrothermal and chemical pretreatments were evaluated. In the combined pretreatment different concentratio...


Chemo-enzymatic saccharification strategy of microalgae chlorella sorokiniana

Constantino, Ana; Rodrigues, Brígida; Raposo, Sara

Biofuel production using microalgae attracted much attention because it can be cultured using CO2 and sunlight. With high carbohydrate content, microalgae have the potential to be used as a fermentation feedstock for bioethanol production. In present work, chemo-enzymatic saccharification of Chlorella sorokiniana microalgae were investigated. Chemical hydrolysis of the biomass followed by enzymatic hydrolysis a...


A new combined approach to improved lipid production using a strictly aerobic a...

Guerreiro, Fábio; Constantino, Ana; Emília Lima-Costa, Maria; Raposo, Sara

Microbial lipids have potential applications in energy, and food industry, because most of those lipids are triacylglycerol with long-chain fatty-acids that are comparable to conventional vegetable oils and can be obtained without arable land requirement. Rhodosporidium toruloides is a strictly aerobic strain, where oxygen plays a crucial role in growth, maintenance, and metabolite production, such as lipids an...


Microalgae growth in different trophic conditions

Constantino, Ana; Jardim, Beatriz; Leon, Antonio; Leon, Rosa; Rodrigues, Brígida; Raposo, Sara

Microalgae growth in different trophic conditions ( Poster Presentation) in Abstract Book 5th Algaeurope Conference


Biofuels production by chlorella sorokiniana in a biorefinery perspective

Constantino, Ana; Glória, Patrícia; Rodrigues, Brígida; Leon, Rosa; Barros, Raúl; Raposo, Sara

Biofuels Production By Chlorella Sorokiniana In A Biorefinery Perspective (Poster Presentation) in Abstract Book 5th Algaeurope Conference


Nitrogen Sources Screening for Ethanol Production Using Carob Industrial Wastes

Raposo, Sara; Constantino, Ana; Rodrigues, F.; Rodrigues, Brígida; Lima-Costa, Maria Emília

Nowadays, bioethanol production is one of the most important technologies by the necessity to identify alternative energy resources, principally when based on inexpensive renewable resources. However, the costs of 2nd-generation bioethanol production using current biotechnologies are still high compared to fossil fuels. The feasibility of bioethanol production, by obtaining high yields and concentrations of eth...


Kinetic and energetic parameters of carob wastes fermentation by Saccharomyces ...

Rodrigues, Brígida; Peinado, J. M.; Raposo, Sara; Constantino, Ana; Quintas, Célia; Emília Lima-Costa, Maria

Carob waste is a useful raw material for the second-generation ethanol because 50% of its dry weight is sucrose, glucose, and fructose. To optimize the process, we have studied the influence of the initial concentration of sugars on the fermentation performance of Saccharomyces cerevisiae. With initial sugar concentrations (S0) of 20 g/l, the yeasts were derepressed and the ethanol produced during the exponenti...


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