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Fatty acids profile of selected Artemisia spp. plants: health promotion

Carvalho, Isabel Saraiva de; Teixeira, Monica; Brodelius, Maria

In the present study we report the fatty acids profile of thirteen species of Artemisia, a hardy herb or shrub, analyzed by gas chromatography connected to a mass detector (GC-MS) for their nutritional value and their potential exploitation as a new source of essential fatty acids. Total lipids content ranged from 3.31 ± 0.19 to 17.78 ± 0.27 mg/g (fresh weight). The three most abundant fatty acids were C16:0, C...


Omega-3 fatty acid desaturase genes isolated from Purslane (Portulaca oleracea ...

Teixeira, Monica; Carvalho, Isabel Saraiva de; Brodelius, Maria

Two full-length cDNA clones PoleFAD7 and PoleFAD8, encoding plastidial omega-3 fatty acid desaturases were isolated from purslane (Portulaca oleracea). The encoded enzymes convert linoleic to alpha-linolenic acid (C18:3n-3). Three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains were identified in the ...


Molecular cloning and expression analysis of three omega-6 desaturase genes fro...

Teixeira, Monica; Coelho, N.; Olsson, M. E.; Brodelius, P. E.; Carvalho, Isabel Saraiva de; Brodelius, Maria

Two full-length cDNA clones of PoleFAD2 and one full-length cDNA clone of PoleFAD6, encoding omega-6 fatty acid desaturases, the key enzymes for the conversion of oleic into linoleic acid, were isolated from purslane (Portulaca oleracea L.) leaves and seeds. The deduced amino acid sequence of both isoforms of PoleFAD2 showed higher similarities to other microsomal omega-6 desaturases then to PoleFAD6 or other p...


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